The Use of MALDI-TOF Mass Spectrometry to Analyze Commensal Oral Yeasts in Nursing Home Residents

Lu, Jang‐Jih; Lo, Hsiu‐Jung; Lee, Chih-Hua; Chen, Meijun; Lin, Chung-Cherng; Chen, Yin-Zhi; Tsai, Ming-Horng; Wang, Shao–Hung

doi:10.3390/microorganisms9010142

Cited by 11 publications

(7 citation statements)

References 50 publications

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“…Cultivation is inevitable when yeasts are identified by MALDI-TOF MS, but the process only requires a minute number of cells, and limited cultivation time. The spectra were analysed using MALDI Biotyper OC version 4.1 (Bruker Daltonik GmbH) and online software MSI (Mass Spectrometry Identification platform) (8). In case of discrepancies between MALDI-TOF and biochemical testing results, we confirmed the ID by DNA-sequencing of ITS region as previously described by our scientific team (9).…”

Section: Methodsmentioning

confidence: 95%

Retrospective Re- Identification of Candida Strains by Maldi-Tof in Seek of Candida Auris

Ivanova¹,

Boyanova²,

Kantardjiev³

2023

Probl Infect Parasit Dis

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The yeast C. auris was first described as a new species in 2009. Since then, this species was recognized as an emerging multi-drug-resistant (MDR) yeast that can cause a wide spectrum of infections, ranging from fungemia to deep-seated infections, especially in intensive care settings. It can be not- or misidentified by commercial identification systems. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently been considered as a convenient, rapid and accurate technology in the identification of yeast isolates to species level. A total of 132 clinical isolates, collected from 2015 to 2021 in the National Reference Laboratory of Mycology and Sexually Transmitted Infections were included in the study. The isolates were mainly from patients with candidemia and other specimens from invasive Candida infections. The isolates were identified by standard mycological procedures, the assimilation profile was done by commercially available strips and with the automated VITEK2 Compact system. All the strains were identified with MALDI-TOF MS. Antifungal susceptibility testing was conducted to some of the strains by agar disk-diffusion (according to CLSI M44-A2), E-test and VITEK2. Some of the strains which could not be identified or were misidentified by standard mycological procedures were correctly determined by MALDI-TOF MS. None of them was identified as C. auris. There were no large deviations in the antifungal susceptibility profiles of the tested strains. We assess MALDI-TOF MS as convenient, rapid, cost- effective and accurate technology in the identification of fungal strains which are difficult to determine with the traditional procedures. Up to now, isolation of C. auris has not been reported in Bulgaria.

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Section: Methodsmentioning

confidence: 95%

Retrospective Re- Identification of Candida Strains by Maldi-Tof in Seek of Candida Auris

Ivanova¹,

Boyanova²,

Kantardjiev³

2023

Probl Infect Parasit Dis

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“…All isolates were subjected to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species identification using MALDI Biotyper software (version 3.1) [ 22 ]. When isolate identifications were inconsistent with the color on CHROMagar Candida agar medium or when uncommon species were reported, the sequences of the internal transcribed spacer (ITS) region and/or the D1/D2 region of ribosomal DNA were used for species identification.…”

Section: Methodsmentioning

confidence: 99%

Distribution of Yeast Species and Risk Factors of Oral Colonization after Oral-Care Education among the Residents of Nursing Homes

Lin

Chiang

et al. 2022

JoF

Self Cite

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Most yeasts causing infections in humans are part of commensal microflora and etiological agents of different infections when hosts become susceptible, usually due to becoming immunocompromised. The colonization of potentially pathogenic microbes in the oral cavity is increased by poor oral hygiene. This follow-up survey was conducted approximately two months after providing information on proper oral care at 10 nursing homes in Taiwan. Among the 117 of 165 residents colonized by yeasts, 67 were colonized by more than one yeast species. A total of 231 isolates comprising eight fungal genera and 25 species were identified. Candida albicans (44.6%) was the dominant species, followed by Candida glabrata (17.7%), Candida parapsilosis (8.7%), Candida tropicalis (7.8%), and Candida pararugosa (7.3%). Residents having a yeast colony-forming unit >10 (OR, 8.897; 95% CI 2.972–26.634; p < 0.001) or using a wheelchair (OR, 4.682; 95% CI 1.599–13.705; p = 0.005) were more likely to be colonized by multiple species. By comparing before and after oral-care education, dry mouth (OR, 3.199; 95% CI 1.448–7.068; p = 0.011) and having heart disease (OR, 2.681; 95% CI 1.068–6.732; p = 0.036) emerged as two independent risk factors for increased density of colonizing yeast.

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“…Candida is a commensal yeast of the oral cavity and may be observed in about 50% of the oropharyngeal region of healthy and non-immunocompromised individuals [4]. C. albicans is the main Candida species in the oral mycobiota, followed by C. glabrata, Candida parapsilosis, Candida tropicalis, and Candida krusei [5,6].…”

Section: Oropharyngeal Candidiasismentioning

confidence: 99%

“…Molecular techniques offer high sensitivity and specificity. These techniques include real-time polymerase chain reaction, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry [5,7].…”

Section: Oropharyngeal Candidiasismentioning

confidence: 99%

Natural Compounds with Antimicrobial Activities in Oral Candida Infections during Head and Neck Radiotherapy

Imperia,

Bonincontro,

Altomare

et al. 2023

Chemotherapy

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Backgrounds: Oral colonization and infections are frequently observed in patients during and soon after radiation therapy (RT). Infective mucositis is a common side effect associated with cancer therapy, characterized by an inflammation of the oral mucous membranes with histological mucosal and submucosal changes. Ulcerative mucositis is responsible for significant pain, impairing the patient's nutritional intake and leading to local or systemic infections promoting mycosis due to several species of the genus Candida. According to international guidelines, treatment of candidiasis depends on the infection site and patient’s condition. Summary: Recently several studies have shown the protective role of natural compounds counteracting the activity of Candida biofilms. The aim of this review is to discuss the antimicrobial activities of natural compounds in fungal infections, especially Candida spp., during and soon after radiotherapy. Indeed new molecules are being discovered and assessed for their capacity to control Candida spp. growth and, probably in the future, will be used to treat oral candidiasis, overall, during radiotherapy. This review reports several preliminary data about preclinical and clinical evidence of their efficacy in the prevention and/or treatment of mucositis due to Radiotherapy with a brief description of the natural compounds with anti-Candida activities. Key messages: The increase in the resistance to the available antifungal drugs related to Candida spp. infections increased as well as drug interactions, urging the development of innovative and more effective agents with antifungal action. Recent preclinical and clinical studies are identifying natural substances with anti-inflammatory and antifungal activity that could be tested in the prevention of candidiasis in patients undergoing radiotherapy. Further studies are needed to confirm these preliminary data.

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The Use of MALDI-TOF Mass Spectrometry to Analyze Commensal Oral Yeasts in Nursing Home Residents

Cited by 11 publications

References 50 publications

Retrospective Re- Identification of Candida Strains by Maldi-Tof in Seek of Candida Auris

Retrospective Re- Identification of Candida Strains by Maldi-Tof in Seek of Candida Auris

Distribution of Yeast Species and Risk Factors of Oral Colonization after Oral-Care Education among the Residents of Nursing Homes

Natural Compounds with Antimicrobial Activities in Oral Candida Infections during Head and Neck Radiotherapy

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