The use of magnetite-doped polymeric microspheres in calibrating cell tracking velocimetry

Moore, Lee R.; Zborowski, Maciej; Nakamura, Masayuki; McCloskey, Kara E.; Gura, Sigalit; Zuberi, Merav; Margel, Shlomo; Chalmers, Jeffrey J.

doi:10.1016/s0165-022x(00)00085-3

Cited by 49 publications

(42 citation statements)

References 15 publications

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“…Recipes for the creation of monodisperse polymer latex and microspheres exist; heterocoagulation of nanoparticles (17)(18)(19) or the direct precipitation of magnetic materials (20)(21)(22) onto the surfaces of monosized latex results in magnetic latex, monodisperse in size. Further polymerization of a polymer layer to cap the latex surface creates hydrophobic (18) or hydrophilic (23) magnetic latex.…”

Section: Introductionmentioning

confidence: 99%

Superparamagnetic Latex via Inverse Emulsion Polymerization

Wormuth

2001

Journal of Colloid and Interface Science

183

117

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Section: Introductionmentioning

confidence: 99%

Superparamagnetic Latex via Inverse Emulsion Polymerization

Wormuth

2001

Journal of Colloid and Interface Science

183

117

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“…We have relied on magnetophoretic mobility as a basis of continuous magnetic cell sorting methods and instrumentation during our past studies [10, 11]. The high sensitivity of the CTV technique to single cell motion allowed us to determine the relationship between surface antigen expression and the immunomagnetic superparamagnetic iron oxide (SPION) label binding [9], and to measure the difference in the MM distributions between RBCs differing in the hemoglobin magnetic moment [5]. The different MM obtained for paramagnetic and non-paramagnetic erythrocytes agreed with values calculated on the basis of bulk hemoglobin content and red blood cell magnetic susceptibility measurements by Gouy’s method [11], superconducting quantum interference device (SQUID) [12] and nuclear magnetic resonance (NMR) methods [13].…”

Section: Methodsmentioning

confidence: 99%

Open Gradient Magnetic Red Blood Cell Sorter Evaluation on Model Cell Mixtures

et al. 2013

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The emerging applications of biological cell separation to rare circulating tumor cell (CTC) detection and separation from blood rely on efficient methods of red blood cell (RBC) debulking. The two most widely used methods of centrifugation and RBC lysis have been associated with the concomitant significant losses of the cells of interest (such as progenitor cells or circulating tumor cells). Moreover, RBC centrifugation and lysis are not well adapted to the emerging diagnostic applications, relying on microfluidics and micro-scale total analytical systems. Therefore, magnetic RBC separation appears a logical alternative considering the high iron content of the RBC (normal mean 105 fg) as compared to the white blood cell iron content (normal mean 1.6 fg). The typical magnetic forces acting on a RBC are small, however, as compared to typical forces associated with centrifugation or the forces acting on synthetic magnetic nanoparticles used in current magnetic cell separations. This requires a significant effort in designing and fabricating a practical magnetic RBC separator. Applying advanced designs to the low cost, high power permanent magnets currently available, and building on the accumulated knowledge of the immunomagnetic cell separation methods and devices, an open gradient magnetic red blood cell (RBC) sorter was designed, fabricated and tested on label-free cell mixtures, with potential applications to RBC debulking from whole blood samples intended for diagnostic tests.

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“…The magnetophoretic mobility of the spores was measured using our in house instrument, Cell Tracking Velocimetry, CTV (Chalmers et al, 1999;Moore et al, 2000;Zhang et al, 2005). Figure 1A presents an overview of the CTV system, while Figure 1B presents an enlarged view of the analysis region where the value of B ranges from 1.5 to 1.875 T, and the gradient, dB/dy, decreases correspondingly to the increase in B such that the product, dB 2 /dy, varies from 0.36 to 0.37 and back down to 0.36 T 2 /mm (between y ¼ À7 and À9 mm, Fig.…”

Section: Methodsmentioning

confidence: 99%

Quantification of magnetic susceptibility in several strains of Bacillus spores: Implications for separation and detection

Melnik

Sun

Fleischman

et al. 2007

Biotech & Bioengineering

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Three strains of Bacillus: Bacillus atrophaeus (formally Bacillus globigii), Bacillus thuringiensis, and Bacillus cereus were tested for their intrinsic magnetic susceptibility. All three strains when sporulated demonstrated significant magnetic susceptibility using an instrument referred to as Cell Tracking Velocimetry. Energy dispersive spectroscopy also confirmed the presence of paramagnetic elements, Fe and Mn, in the spore form of the bacteria. It was demonstrated that this magnetic susceptibility is sufficient to separate and deposit these spores on glass slides in a magnetic deposition system. These results indicate the potential to separate spores with intrinsic magnetic susceptibility directly out of water or air samples.

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The use of magnetite-doped polymeric microspheres in calibrating cell tracking velocimetry

Cited by 49 publications

References 15 publications

Superparamagnetic Latex via Inverse Emulsion Polymerization

Superparamagnetic Latex via Inverse Emulsion Polymerization

Open Gradient Magnetic Red Blood Cell Sorter Evaluation on Model Cell Mixtures

Quantification of magnetic susceptibility in several strains of Bacillus spores: Implications for separation and detection

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