The use of &lt;sup&gt;32&lt;/sup&gt;P Method to Evaluate the Growth of Lowland Rice Cultivated in a System of Rice Intensification (SRI)

Citraresmini, Ania; Anas, Iswandi; Nurmayulis, Nurmayulis

doi:10.17146/aij.2013.236

Cited by 2 publications

(8 citation statements)

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“…Radioisotope 32 P was applied by injecting a 10 ml KH 2 32 PO 4 solution into a leaf axil with a specific activity of 5 mCi or activity type 220 g / uCi (microCurrie). This means that there are 220 g of 32 P in each microCurrie radioactive activity of KH 2 32 PO 4 solution [11]. The first seedling is injected with KH 2 32 PO 4 solution at a lower point of leaf axil.…”

Section: Aplication and Analysismentioning

confidence: 99%

“…Furthermore, the dismantled plants were smashed at 500 o C for 5 hours, then cleaned using HCl 2N solution. 32 P activity was counted/chopped with a liquid scintillation counter (LSC) using Cherenkov calculation [11].…”

Section: Aplication and Analysismentioning

confidence: 99%

“…To ensure that phosphorus can be applied through the leaf axil, it is necessary to prove it using a 32 P tracking technique that is added to the fertilizer. Radioactive tracers have long been used extensively to study nutrient translocation from soil to plant tissue [10,11]. It was also reported by Safuan et al [1] that tracer techniques are widely used to measure P uptake in phosphate rock studies and provide a more reliable availability index than conventional methods and they correlate better with measurements of chemical extraction.…”

Section: Introductionmentioning

confidence: 99%

“…P nutrient tracer technique using 32 P isotope can be done directly or indirectly [11,12]. The direct method is isotope as the tracer is mixed into fertilizer material or other P sources.…”

Section: Introductionmentioning

confidence: 99%

“…The novelty of this research was that there has been no study of the tracing of P translocation patterns from leaf axil to other organs in oil palm plants. In other plants translocation of P from the soils to the plants may have been reported [11][12][13][14][15] or from the leaves in a foliar fertilizer application. With the same analogy to foliar fertilizing, there was hope that monitoring of nutrient travel from the leaf axil to photosynthetic organs can be carried out.…”

Section: Introductionmentioning

confidence: 99%

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Isotopic Tracing of Phosphorus Uptake in Oil Palm Seedlings Leaf Axil Using 32P Labelled

Mildaryani¹,

Mujiyo²,

Dewi³

et al. 2020

Int. J. Adv. Sci. Eng. Inf. Technol.

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Pot experiments have been carried out to investigate the distribution pattern of Phosphorus in oil palm seedlings axillary axes using 32 P radioisotope technique. The experiment was conducted in the greenhouse at the Center for Application of Isotope and Radiation -National Nuclear Energy Agency, Jakarta, Indonesia. KH 2 32 PO 4 solution with the activity of 5 mCi was injected into five points viz through the lowest, middle, upper leaf axil, at the base of the trunk and the soil 5 cm from the trunk. The method used was randomized complete block design with two replications.The 32 P uptake is expressed in counted per minute (cpm) counted by Liquid Scintillation Counter then transformed to disintegration per minute (dpm). The results showed that at the injection point in the lowest leaf axil, the distribution of 32 P was more towards the crown before heading towards the roots. In the middle of the leaf axil, the distribution of 32 P was recorded higher, nutrients were actively distributed to the top of the plant while the roots were very low. The dynamics of 32 P at the upper axil injection point, the highest 32 P uptake at the start of the injection was seen in the lowest leaf axil. The 32 P distribution then leads to the stem, roots and finally to the canopy. At the injection point in the soil 5 cm from the trunk, the counted value shows 32 P distributed to the top of the plant, while at the base of the trunk, 32 P is distributed to the farthest injection point, the plant canopy. From this study, it can be concluded that 32 P radioisotopes can describe the distribution of nutrients in plant parts, wherever radioisotope is applied. Phosphorus can be applied through any leaf axil and can be distributed well to the canopy of plants.

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Section: Aplication and Analysismentioning

confidence: 99%

Section: Aplication and Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…P nutrient tracer technique using 32 P isotope can be done directly or indirectly [11,12]. The direct method is isotope as the tracer is mixed into fertilizer material or other P sources.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isotopic Tracing of Phosphorus Uptake in Oil Palm Seedlings Leaf Axil Using 32P Labelled

Mildaryani¹,

Mujiyo²,

Dewi³

et al. 2020

Int. J. Adv. Sci. Eng. Inf. Technol.

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Allocation of Phosphorus Fractions in Chinese Fir in Response to Low Phosphorus Availability Using 32P Tracer

Zou

Liu

Huang

et al. 2022

Forests

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Phosphorus (P) is among the most intractable constraints on plant fertility, particularly in acidic soils with high P fixation capacities. The effects of nutrient limitation and the adaptive strategies of plants in infertile soils are central topics in plant ecology. The development of tree cultivars with greater P use efficiency (PUE), defined as the ability of a tree to grow and be productive in soils with reduced P availability, would substantially improve forest development. The ability of plants to redistribute and transfer P across fractions determines their adaptability to P limitations. However, the mechanisms of P utilization and transport remain unknown in Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) from the perspective of P fraction distribution. In this study, we investigated the distribution and translocation patterns of exogenous P and different P fractions in the M1 Chinese fir, which was identified as exhibiting high P-deficient resistance ability and maintaining higher yield under low P stress relative to the average clones, using 32P tracking, which can accurately trace the migration pathways of exogenous P after plant absorption. We found that exogenous P in the roots was higher than in the stems or leaves under low-P conditions in which the amount of the exogenous P absorbed by M1 was significantly reduced. Under low-P conditions, the plants optimized P allocation, which led to higher PUE than under high-P conditions, with the highest PUE in the leaves, followed by the stems and roots. The M1 clone maintained a high ratio of soluble P (i.e., inorganic P and ester P) in its leaves and stems, which improved P mobility and recycling under the conditions of limited P. In the roots, the P fractions shifted from soluble inorganic P and ester P to insoluble P (i.e., nucleic P), but the total P concentration was relatively stable, which may ensure root growth and exogenous P absorption under the conditions of limited P. Our results confirm that the M1 Chinese fir reduces P demand, optimizes the allocation of P among P fractions, and increases PUE to maintain aboveground productivity in response to limited P conditions.

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The use of <sup>32</sup>P Method to Evaluate the Growth of Lowland Rice Cultivated in a System of Rice Intensification (SRI)

Cited by 2 publications

References 1 publication

Isotopic Tracing of Phosphorus Uptake in Oil Palm Seedlings Leaf Axil Using 32P Labelled

Isotopic Tracing of Phosphorus Uptake in Oil Palm Seedlings Leaf Axil Using 32P Labelled

Allocation of Phosphorus Fractions in Chinese Fir in Response to Low Phosphorus Availability Using 32P Tracer

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