The use of <i>R-nj, B1, Pl1</i> genes to improve marker properties in the selection of maize haploinducers

Abstract

BACKGROUND: Studies were carried out on the creation of haploinductor lines using various methods for testing haploinductor and marker ability from the VIR maize genetic collection, adapted to the conditions of the Liaoning province in China and the South of Russia.&#x0D; MATERIALS AND METHODS: On 22 maize testers, studies were carried out on the creation of haploinductor lines and testing the haploinductor and marker ability by the test-cross method. Sources of R1-nj, B1, Pl1 genes from the VIR maize genetic collection were used.&#x0D; RESULTS: The lines of maize haploinducers Liaoyu No. 1, Liaoyu No. 2, Liaoyu No. 3 and Liaoyu No. 4 were created with a haploinduction frequency of 6.85%, 7.53%, 6.66% and 6.03%. respectively, with a high quality of labeling with the R1-nj, B1, Pl1 genes in test crosses.&#x0D; CONCLUSIONS: In the process of selection of haploid maize on the basis of genes A, haploinductor lines with a high frequency of haploinduction and high efficiency of markers in test crosses, abundant pollen and grain productivity, resistance to late blight and lodging, and adaptation to the agroclimatic zone of Shenyang Province (China) were obtained and the North Caucasian Federal District (Russia).

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

“…В настоящее время продолжается совершенствование существующих линий-гаплоиндукторов и поиск новых генов, способствующих повышению частоты гаплоидии (Ulyanov et al, 2022).…”

“…Гаплоидия широко применяется для ускорения гибридной селекции и получения новых линий кукурузы с улучшенными признаками и их стерильных аналогов (Gutorova et al, 2016;Liu et al, 2022). Гаплоиндукторные линии кукурузы и их тетраплоидные аналоги используются в селекции редиплоидных линий кукурузы методом ресинтеза из тетраплоидных форм (Khatefov, Shatskaya, 2007;Khatefov et al, 2019;.…”