The use of laser Doppler blood flow to assess the effect of acute administration of vitamin D on micro vascular endothelial function in people with diabetes

Abstract

Objective:To assess the effect of vitamin D administration on the skin blood flow response to occlusion and heat. Design: Cross-sectional study. Methods: Twenty age matched subjects; 10 who had diabetes and 10 who were controls were administered 4,000 IU of vitamin D3 for 3 weeks at breakfast. The function of the endothelial cells was evaluated in 2 ways; first, the response to 4 minutes of vascular occlusion of the skin was measured with a laser Doppler flow meter. Second, the skin blood flow response to local heat at 42 degrees C for 6 minutes was examined. Results:The results of the experiments showed that the blood flow response to heat was reduced after 3 weeks administration of vitamin D in the subjects with diabetes and in the control subjects (p<0.05). The response to occlusion was not significantly different within each group before and after vitamin D administration, but the group with diabetes had a significantly lower blood flow response to occlusion than did the controls (p<0.05). Conclusions: Acute doses of vitamin D may impair nitric oxide production and reduce blood flow to tissue during stressors in people with diabetes.

Evaluating the Far-Infrared Radiation Bioeffects on Micro Vascular Dysfunction, Nervous System, and Plantar Pressure in Diabetes Mellitus

“…The non-contact blood flow imaging system (Moor Instruments Inc, Moor FLPI-2 Devon, UK) can detect 38 cm deep and 15 cm × 20 cm area to analyze the blood flow in the foot (right instep, left instep, right sole, and left sole). 19,20 Plantar pressure test system used was RSscan Footscan pressure Pad (RSscan Int, Olen, Belgium). 21,22 The effective test area of this pressure plate is 48.8 cm × 32.5 cm.…”