The use of Lactobacillus brevis PS1 to in vitro inhibit the outgrowth of Fusarium culmorum and other common Fusarium species found on barley

Mauch, Alexander; Bello, Fabio Dal; Coffey, Aidan; Arendt, Elke K.

doi:10.1016/j.ijfoodmicro.2010.05.002

Cited by 67 publications

(39 citation statements)

References 34 publications

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“…plantarum 16 were shown to affect growth of R. stolonifer and A. fumigatus when applied in agar and liquid growth media. In keeping with other findings (Guo et al, 2011;Mauch et al, 2010), the application of antifungal supernatant from lactobacilli resulted in the inhibition of germ tube formation, as determined by microscopic analysis. Furthermore, the addition of antifungal supernatant from Lb.…”

Section: Discussionsupporting

confidence: 87%

“…Moreover, the molecular target(s) and mode(s) of action of these bioactive compounds have not yet been identified. Previous studies have attempted to discern the interactions between antifungal LAB and their targeted fungi (Mauch et al, 2010;Ström et al, 2005). The effects of Lactobacillus plantarum MiLAB 393 on the fungal growth and protein expression of Aspergillus nidulans were investigated by Ström et al (2005).…”

Section: Introductionmentioning

confidence: 99%

“…The study revealed the alteration in expression of two groups of proteins, as observed by 2D PAGE, upon treatment with three antifungal compounds produced by this bacterium. Microscopic investigations have also attempted to shed some light on these enigmatic interactions (Lavermicocca et al, 2003;Mauch et al, 2010). Germ tube formation and subsequent hyphal development of Trichophyton tonsurans were inhibited by the addition of concentrated cell-free supernatant (cCFS) from Lactobacillus reuteri R2 (Guo et al, 2011), while brevicin SG1 (produced by Lactobacillus brevis SG1) induced lysis and disruption of the cell wall of Penicillium citrinum and reduced hyphal branching in Candida albicans (Adebayo & Aderiye, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic and morphological profiling of Aspergillus fumigatus Af293 in response to antifungal activity produced by Lactobacillus plantarum 16

et al. 2013

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The morphological effects of an antifungal activity produced by Lactobacillus plantarum 16, a malt-production steep water isolate, on two food-associated fungi were examined microscopically. Spore germination was completely inhibited in Aspergillus fumigatus and Rhizopus stolonifer upon treatment with concentrated cell-free supernatant (cCFS) of strain 16. Furthermore, addition of antifungal cCFS to germ tubes and hyphae halted further development compared to untreated controls. Transcriptome analysis of A. fumigatus Af293 following exposure to antifungal cCFS revealed a number of genes with altered transcription involved in a variety of cellular functions, most notably cell metabolism, suggesting a global metabolic shutdown and subsequent cell death. Increased transcription of the global regulator LaeA was also observed indicating that exposure to the antifungal activity caused a cellular stress response.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic and morphological profiling of Aspergillus fumigatus Af293 in response to antifungal activity produced by Lactobacillus plantarum 16

et al. 2013

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“…Mould can cause high rates of mortality among fruit fly larvae, and groups of larvae can suppress mould growth as well as enhance the growth of certain yeast species (Rohlfs et al, 2005;Stamps et al, 2012). Intriguingly, both species of Lactobacillus we have studied produce compounds that suppress fungal and bacterial growth (Ruiz-Barba et al, 1994;Laitila et al, 2002;Schnürer and Magnusson, 2005;Mauch et al, 2010;Crowley et al, 2012). The fruit fly microbiome may thus help larvae control the species composition of fungi and bacteria on their fruit substrates.…”

Section: Benefits Of the Microbiomementioning

confidence: 99%

Social attraction mediated by fruit flies' microbiome

Venu

Durisko

et al. 2014

Journal of Experimental Biology

103

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Larval and adult fruit flies are attracted to volatiles emanating from food substrates that have been occupied by larvae. We tested whether such volatiles are emitted by the larval gut bacteria by conducting tests under bacteria-free (axenic) conditions. We also tested attraction to two bacteria species, Lactobacillus brevis, which we cultured from larvae in our lab, and L. plantarum, a common constituent of fruit flies' microbiome in other laboratory populations and in wild fruit flies. Neither larvae nor adults showed attraction to axenic food that had been occupied by axenic larvae, but both showed the previously reported attraction to standard food that had been occupied by larvae with an intact microbiome. Larvae also showed significant attraction to volatiles from axenic food and larvae to which we added only either L. brevis or L. plantarum, and volatiles from L. brevis reared on its optimal growth medium. Controlled learning experiments indicated that larvae experienced with both standard and axenic used food do not perceive either as superior, while focal larvae experienced with simulated used food, which contains burrows, perceive it as superior to unused food. Our results suggest that flies rely on microbiome-derived volatiles for longdistance attraction to suitable food patches. Under natural settings, fruits often contain harmful fungi and bacteria, and both L. brevis and L. plantarum produce compounds that suppress the growth of some antagonistic fungi and bacteria. The larval microbiome volatiles may therefore lead prospective fruit flies towards substrates with a hospitable microbial environment.

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“…Lactic acid bacteria (LABs) are a known potential source for generating a variety of secondary metabolites such as bacteriocines, organic acids and peptides (Cizeikiene, Juodeikiene, Paskevicius, & Bartkiene, 2013). In the past decade the interest for antifungal LABs has increased and different studies have showed that many LAB strains have the potential to combat the proliferation of fungi in various food and feed materials (Rouse, Harnett, Vaughan, & Van Sinderen, 2008;Dalie, Deschamps, & Richard-Forget, 2010;Mauch, Dal Bello, Coffey, & Arendt, 2010;Gerez, Torino, Rollan & De Valdez, 2009;Dal Bello et al, 2007). Consumers are demanding the replacement of artificial chemical preservatives by natural biopreservatives to reduce fungal contamination in foods (Brul & Coote, 1999;Crowley, Mahony, & Van Sinderen, 2013;Reis, Paula, Casarotti, & Penna, 2012;Schnurer & Magnusson, 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

Luz¹,

Saladino²,

Luciano³

et al. 2017

LWT - Food Science and Technology

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a b s t r a c tFood spoilage caused by mycotoxigenic moulds represents an important problem in food security. The antimicrobial peptides are compounds of natural origin constituted by a variable number (5e100) of amino acids held together through peptide bonds. In this work, the cell free supernatants (CFSs) containing peptides obtained from four strains of LAB were lyophilized, filtered and tested to determine the antifungal activity against Aspergillus Parasiticus and Penicillium expansum. CFS obtained by Lactobacillus plantarum showed the highest inhibition activity. CFS was fractionated by size exclusion chromatography and injected into the liquid chromatography coupled to diode array detector. One of the recollected fractions resulted interesting for the presence of three peaks that were purified by the technique of the LC-DAD using a semi preparative C18 column. Finally, the antifungal activity of the purified peptides was studied against A. Parasiticus and P. expansum in liquid medium. The MALDI-TOF/TOF mass spectrometry was used for the peptides identification. The three purified peptides presented an amino acidic sequence identified by a bioinformatics program of SGADTTFLTK, LVGKKVQTF, and GTLIGQDYK. The first peptide purified reduced 58% and 73% the growth of P. expansum and A. parasiticus, respectively, in liquid medium after 48 h incubation.

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The use of Lactobacillus brevis PS1 to in vitro inhibit the outgrowth of Fusarium culmorum and other common Fusarium species found on barley

Cited by 67 publications

References 34 publications

Transcriptomic and morphological profiling of Aspergillus fumigatus Af293 in response to antifungal activity produced by Lactobacillus plantarum 16

Transcriptomic and morphological profiling of Aspergillus fumigatus Af293 in response to antifungal activity produced by Lactobacillus plantarum 16

Social attraction mediated by fruit flies' microbiome

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

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