The Use of Ivermectin to Kill <i>Ixodes Scapularis</i> Ticks Feeding on Humans

Sheele, Johnathan M.; Ford, Lucie; Tse, Adele; Chidester, Benjamin; Byers, Peter A.; Sonenshine, Daniel E.

doi:10.1016/j.wem.2013.09.008

Cited by 7 publications

(3 citation statements)

References 13 publications

(69 reference statements)

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“…In our previous study, it was demonstrated that IVM and AVM of the AVM family not only inhibited the proliferation of glioma cells via evoking apoptosis but also induced autophagy in glioma cells ( 21 , 48 ). Furthermore, IVM has been used to treat filariasis and kill ixodesscapularis ticks feeding on humans ( 49 ). In the present study, DRM, a more effective and less toxic drug from the AVM family, was used to treat GBM.…”

Section: Discussionmentioning

confidence: 99%

Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo

et al. 2022

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Glioblastoma (GBM) is one of the most widespread and lethal types of cancer. However, there are currently no drugs or therapeutic strategies that can completely cure GBM. Doramectin (DRM) has a broad range of activities against endoparasites and ectoparasites, and is extensively used in livestock. In the present study, the effect of DRM on the induction of autophagy in U87 and C6 GBM and glioma cell lines, as well as the mechanism of autophagy, were examined. First, transmission electron microscopy, plasmid transfection and western blot analysis demonstrated that DRM could induce autophagy in U87 and C6 cells in vitro. Next, MTT and colony formation assays revealed that DRM-induced autophagy prevented U87 and C6 cell viability and colony formation ratio. In addition, DRM-induced autophagy promoted U87 and C6 cell apoptosis, as indicated by DAPI analysis and flow cytometry. Furthermore, transcriptome analysis demonstrated that DRM modulated a number of genes and pathways involved in autophagy. In a nude mouse xenograft model, immunohistochemical staining and the TUNEL assay demonstrated that the effect of DRM on the tumor was consistent with that in vivo. These data indicated that DRM induced autophagy mainly by blocking the PI3K/AKT/mTOR signaling pathway in GBM cells. DRM-induced autophagy promoted the inhibition of GBM cell proliferation and apoptosis in vitro and in vivo. The present study suggested that DRM may be an effective drug for the treatment of GBM.

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Section: Discussionmentioning

confidence: 99%

Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo

et al. 2022

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“…70 However, in a case series, tick removal with forceps was associated with a lower rate of spirochetal and rickettsial infections. 71 Passive methods such as application of petroleum jelly, fingernail polish, 70% isopropyl alcohol, or a hot kitchen match, 67,72 local infiltration of anesthetics, 73 and administration of oral ivermectin 74 failed to exhibit any efficacy in encouraging tick detachment. Overall, mechanical removal is largely accepted by experts, 75 and forceps removal has been endorsed by the CDC (Figure 5) 8 ; however, to date no professional organization has adopted strong practice guidelines regarding optimal tick removal techniques.…”

Section: Tick Removalmentioning

confidence: 99%

Wilderness Medical Society Clinical Practice Guidelines for the Prevention and Management of Tick-Borne Illness in the United States

Ho¹,

Davis

Forrester

et al. 2021

Wilderness & Environmental Medicine

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The Wilderness Medical Society convened an expert panel to develop evidence-based guidelines for the prevention and management of tick-borne illness. Recommendations are graded based on quality of supporting evidence according to criteria put forth by the American College of Chest Physicians. The guidelines include a brief review of the clinical presentation, epidemiology, prevention, and management of tick-borne illness in the United States, with a primary focus on interventions that are appropriate for resource-limited settings.

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“…Nowadays, IVM is used mainly as an oral medication in the primary treatment of onchocerciasis, lymphatic filariasis, and strongyloidiasis infections, all caused by different nematode species ( Turner and Schaeffer, 1989 ; Laing et al., 2017 ). IVM also affects arthropod ectoparasites, being used in the treatment of scabies ( Thomas et al., 2015 ), ticks ( Sheele et al., 2014 ), and head lice ( Devore and Schutze, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Broadening the spectrum of ivermectin: Its effect on Trypanosoma cruzi and related trypanosomatids

Fraccaroli

Ruiz

Perdomo

et al. 2022

Front. Cell. Infect. Microbiol.

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Chagas disease is an endemic American parasitosis, caused by Trypanosoma cruzi. The current therapies, benznidazole (BZN) and nifurtimox (NFX), show limited efficacy and multiple side effects. Thus, there is a need to develop new trypanocidal strategies. Ivermectin (IVM) is a broad-spectrum antiparasitic drug with low human and veterinary toxicity with effects against T. brucei and Leishmania spp. Considering this and its relatively low cost, we evaluate IVM as a potential repurposed trypanocidal drug on T. cruzi and other trypanosomatids. We found that IVM affected, in a dose-dependent manner, the proliferation of T. cruzi epimastigotes as well as the amastigotes and trypomastigotes survival. The Selectivity Index for the amastigote stage with respect to Vero cells was 12. The IVM effect was also observed in Phytomonas jma 066 and Leishmania mexicana proliferation but not in Crithidia fasciculata. On the epimastigote stage, the IVM effect was trypanostatic at 50 μM but trypanocidal at 100 μM. The assays of the drug combinations of IVM with BNZ or NFX showed mainly additive effects among combinations. In silico studies showed that classical structures belonging to glutamate-gated Cl channels, the most common IVM target, are absent in kinetoplastids. However, we found in the studied trypanosomatid genomes one copy for putative IMPα and IMPβ, potential targets for IVM. The putative IMPα genes (with 76% similarity) showed conserved Armadillo domains but lacked the canonical IMPβ binding sequence. These results allowed us to propose a novel molecular target in T. cruzi and suggest IVM as a good candidate for drug repurposing in the Chagas disease context.

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The Use of Ivermectin to Kill Ixodes Scapularis Ticks Feeding on Humans

Abstract: There were a high number of ticks that died in both groups but the data do not support our hypothesis that ivermectin can kill I scapularis. The study was not designed to determine whether it could prevent the transmission of tick-borne illness.

Cited by 7 publications

References 13 publications

Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo

Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo

Wilderness Medical Society Clinical Practice Guidelines for the Prevention and Management of Tick-Borne Illness in the United States

Broadening the spectrum of ivermectin: Its effect on Trypanosoma cruzi and related trypanosomatids

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