The Use of Incident Light in Immunofluorescence Applied to Syphilis Serology

Kasatiya, S. S.; Birry, A

doi:10.1093/ajcp/57.3.395

Cited by 6 publications

(3 citation statements)

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“…The patient's serum (0.02 ml) and controls at a dilution of 1:16 were placed on precoded Redi-fix slides and processed accordingly. The processed slides were inverted and read as described previously (8), using a Leitz Ortholux microscope fitted with a Ploem illuminator. No oil between the condenser and the slide or the slide and the objective was used.…”

Section: Methodsmentioning

confidence: 99%

Immunofluorescent antibody test for diagnosis of gonorrhoea

Caloenescu¹,

Clecner²,

Petrow³

et al. 1975

J Clin Microbiol

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An indirect fluorescent antibody test was evaluated in 198 cases of a high-risk group with a culture prevalence of 37.3% and in 426 cases of a low-risk group with a culture prevalence of 1.16%. A sensitivity of 77.1% in the culture-positive patients with uncomplicated gonorrhoea, and a specificity of 88.7% in the culture-and history-negative cases, was obtained in the high-risk group. In this group, the sera from 88.8% of the patients with culture-proven gonorrhoea became positive in an indirect fluorescent antibody test within 3 weeks of last sexual contact. In the low-risk group, for which the sensitivity could not be determined due to various reasons, a specificity of 95.8% was obtained. Complement fixation test was positive in sera of only 17.6% of the culture-positive cases of the high-risk group.

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Section: Methodsmentioning

confidence: 99%

Immunofluorescent antibody test for diagnosis of gonorrhoea

Caloenescu¹,

Clecner²,

Petrow³

et al. 1975

J Clin Microbiol

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show abstract

“…Monospecific IgG conjugate was used in a dilution of 1/2300 with unabsorbed CSF and of 1/600 with absorbed CSF to get the same intensity of fluorescence with strongly reactive, weakly reactive, and non-reactive CSF controls selected in the laboratory. A Leitz Orthoplan microscope equipped with an incident light system was used according to the method described by Kasatiya and Birry (1972).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of microhaemagglutination assay to determine treponemal antibodies in CSF.

Birry¹,

Kasatiya²

1979

Sexually Transmitted Infections

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“…A halogen lamp (Sylvania, 100 W, FCR) was used in substage illumination for specimen location and focusing. The excitation sources used in the present study were a UV bulb (Osram G.M.B.H., Berlin, Germany; HBO 100) in incident illumination (2,8) and a pulsed dye laser (Synergetics Research, Princeton, N.J.; Chromabeam model 1070) in transmitted substage illumination. A helium-neon CW laser (Metrologic Instruments, Inc., Belmawr, N.J.; model 360) with low output was used for optical alignment of the pulsed dye laser.…”

mentioning

confidence: 99%