The use of image analysis to study developmental variation in micropropagated potato (Solanum tuberosum L.) plants

Cassells, A. C.; Kowalski, Britta; Fitzgerald, Donna M.; Murphy, Gwen

doi:10.1007/bf02358170

Cited by 15 publications

(6 citation statements)

References 9 publications

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“…the lower concentrations tested had no effect on yield parameters (table 1, Figure 1). Some authors showed that the quality of in vitro plants has influenced acclimatization and subsequent development of plantlets in the field (Cassells et al 1999. a good physiological condition of in vitro plants is a guarantee of the later successful seed production process in greenhouse.…”

Section: Resultsmentioning

confidence: 99%

Effect of in vitro chitosan application on growth and minituber yield of Solanum tuberosum L.

Zakaria¹,

Maleki-Zanjani²,

Sedghi³

2009

Plant Soil Environ.

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ABSTRACTin order to investigate the effects of soluble chitosan on plantlets growth in vitro and increase of minituber yield in potato micropropagation, plantlets of agria cultivar were treated in vitro with soluble chitosan at different concentrations including 0, 5, 15, 50, 150, 500, 750 and 1000 mg/l added to the MS tissue culture medium. Plantlets were subsequently transferred to the greenhouse and minituber yield parameters were evaluated. at the concentrations of 750 and 1000 mg/l of chitosan the culture medium failed to solidify. application of 500 mg/l of soluble chitosan increased the shoot fresh weight, but its lower concentrations did not significantly affect this trait (P < 0.05). The 5 and 15 mg/l of soluble chitosan led to a significant increase in root fresh and dry weight of in vitro plantlets, whereas, higher concentrations, especially 500 mg/l, significantly decreased root fresh weight of in vitro plantlets. application of 500 mg/l chitosan in vitro resulted in improved acclimatization of plantlets in the greenhouse as expressed by significant (P < 0.05) increase in minituber number and yield, compared to the control. The tested lower concentrations had no effect on yield parameters. The present results indicate that soluble chitosan can be successfully incorporated into potato seed production from in vitro plantlets.

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Section: Resultsmentioning

confidence: 99%

Effect of in vitro chitosan application on growth and minituber yield of Solanum tuberosum L.

Zakaria¹,

Maleki-Zanjani²,

Sedghi³

2009

Plant Soil Environ.

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“…Plant quality in vitro has been shown to influence subsequent development of plantlets at acclimatisation and in the field (Cassells et al 1999;Kowalski et al 1999aKowalski et al , b, 2003. Apparently, a favourable physiological condition can be established in the in vitro phase, which is then carried through to the later stages of the seed production process in greenhouse and field.…”

Section: Discussionmentioning

confidence: 98%

Application of Soluble Chitosan in vitro and in the Greenhouse to Increase Yield and Seed Quality of Potato Minitubers

et al. 2007

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The aim of this work was to investigate whether the application of soluble chitosan in potato micropropagation can improve microplant quality in vitro, help acclimatisation ex vitro, and increase yield and seed quality of minitubers. Potato cv. Désirée microplants were treated in vitro with soluble chitosan added to the semisolid tissue culture medium in different concentrations. Microplants were subsequently transferred to the greenhouse and sprayed with chitosan solutions or remained unsprayed. Untreated microplants were also established ex vitro and sprayed with chitosan, or left unsprayed as a control. Morphological and physiological parameters of plant growth were assessed in vitro and ex vitro. Plantlet quality was evaluated using a ranking system. Minitubers derived from greenhouse plantlets were planted in the field in subtropical conditions, and growth and yield parameters evaluated. The chitosan concentration most beneficial to the in vitro growth of microplants varied between years. In treatments with the best in vitro growth, minituber number and yield in the greenhouse was also increased. Foliar chitosan application at the acclimatisation phase stabilised the effect of in vitro treatment on yield parameters. The seed quality of minitubers derived from chitosan treatments in vitro alone and in combination with foliar treatment at acclimatisation was improved, giving rise to field plants with increased tuber numbers and yields. The combination of chitosan in vitro and foliar application during the acclimatisation phase ex vitro had a greater effect on minituber seed quality than the concentration applied in vitro.

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“…It's made from chitin shells of shrimp, crabs and other crustaceans with an alkaline substance and is also found in both fungal and insect cell walls W alker-Simmons et al (1983). It's also the antifungal agent against plant disease resistance and nontoxic effect on the environment (Vander et al 1998;Benhamou et al 1994;El-Ghaouth et al 1994, Cassells et al 1999Roby et al 1987 andTiuterev et al 1996). Chitosan has so many properties like growth stimulator, antimicrobial properties, plant disease resistance, biodegradability, biocompatible for the environment, etc Uthairatanakij et al (2007).…”

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confidence: 99%

Chitosan as a Growth Promoter and Enhance Survival Rate in an in vitro Culture of Banana (Musa spp.) Cultivar ‘Bantala’

Kandha

Kumar

Bindhani

2022

IJARe

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Background: Banana (Musa spp.) is one of the most consumable fruits and cultivated around the globe. It contains high nutritional value as well as the high demand of the market. The microbes are the main problem for the propagation of banana plants in tissue culture. Chitosan is one of the best substances for the eradication of contamination and also growth stimulators of banana plants. This study is based on the micro-propagation of the bantala variety of Musa species and free from microbe infection. Methods: The rhizome and sucker as explants of Musa cv. Bantala. The different combination concentrations of 6-Benzylaminopurine (BAP), indole-3-acetic acid (IAA) and chitosan (CS) were tried in Murashige and Skoog medium for in vitro response of plants, shoot initiation and shoot proliferation. The formation of rooting was used as the half-strength Murashige and Skoog (MS) medium with indole-3-butyric acid (IBA) and chitosan (CS). Result: The best response, shoot initiation and shoot proliferation were observed at 6-Benzylaminopurine (5.0 mg/L)+indole-3-acetic acid (0.5 mg/L)+chitosan (25 mg/L) and 6-Benzylaminopurine (4.0 mg/L)+indole-3-acetic acid (0.5 mg/L)+chatoyant (25 mg/L) in both of rhizome and sucker respectively. The maximum root formations were observed in the medium containing half-strength Murashige and Skoog medium+1.0 mg/L indole-3-butyric acid+25 mg/L chitosan in the rhizome and 0.8 mg/L indole-3-butyric acid+25 mg/L chitosan in the sucker. The successful survival rate of sucker and rhizome under the acclimatization condition was recorded as 90% and 88% compared with control as 66% and 63% respectively. This standardized protocol might be useful for the mass production of bantala variety as well as other cultivars of banana plants.

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The use of image analysis to study developmental variation in micropropagated potato (Solanum tuberosum L.) plants

Cited by 15 publications

References 9 publications

Effect of in vitro chitosan application on growth and minituber yield of Solanum tuberosum L.

Effect of in vitro chitosan application on growth and minituber yield of Solanum tuberosum L.

Application of Soluble Chitosan in vitro and in the Greenhouse to Increase Yield and Seed Quality of Potato Minitubers

Chitosan as a Growth Promoter and Enhance Survival Rate in an in vitro Culture of Banana (Musa spp.) Cultivar ‘Bantala’

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