The use of<i>in vivo</i>zebrafish assays in drug toxicity screening

Eimon, Peter M.; Rubinstein, Amy L.

doi:10.1517/17425250902882128

Cited by 118 publications

(63 citation statements)

References 45 publications

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“…63,114 For many years, researchers have been using zebrafish assays for drug toxicity screening with a view to provide early drug safety assessments. 115,116 So far, zebrafish have been used to predict drug-related cardiotoxicity, ototoxicity, developmental toxicity, neurotoxicity, oculotoxicity, and many more. 115,116 Furthermore, zebrafish have efficiently predicted toxicity associated with the effects of systemic drugs in humans.…”

Section: Drug-related Ophthalmological Toxicity Assessment In Zebrafishmentioning

confidence: 99%

“…115,116 So far, zebrafish have been used to predict drug-related cardiotoxicity, ototoxicity, developmental toxicity, neurotoxicity, oculotoxicity, and many more. 115,116 Furthermore, zebrafish have efficiently predicted toxicity associated with the effects of systemic drugs in humans. 116 More recently, a study described zebrafish as a potential animal model to predict drug-related oculotoxicity at the preclinical stage.…”

Section: Drug-related Ophthalmological Toxicity Assessment In Zebrafishmentioning

confidence: 99%

“…115,116 Furthermore, zebrafish have efficiently predicted toxicity associated with the effects of systemic drugs in humans. 116 More recently, a study described zebrafish as a potential animal model to predict drug-related oculotoxicity at the preclinical stage. 114 In this study, 3 dpf zebrafish larvae were exposed to the known oculotoxic drugs (digoxin, gentamicin, ibuprofen, minoxidil, and quinine) for 48 h, and toxicity was assessed using visual behaviour.…”

Section: Drug-related Ophthalmological Toxicity Assessment In Zebrafishmentioning

confidence: 99%

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Zebrafish—on the move towards ophthalmological research

Chhetri

Jacobson

Gueven

2014

Eye

129

106

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Millions of people are affected by visual impairment and blindness globally, and the prevalence of vision loss is likely to increase as we are living longer. However, many ocular diseases remain poorly controlled due to lack of proper understanding of the pathogenesis and the corresponding lack of effective therapies. Consequently, there is a major need for animal models that closely mirror the human eye pathology and at the same time allow higher-throughput drug screening approaches. In this context, zebrafish as an animal model organism not only address these needs but can in many respects reflect the human situation better than the current rodent models. Over the past decade, zebrafish have become an established model to study a variety of human diseases and are more recently becoming a valuable tool for the study of human ophthalmological disorders. Many human ocular diseases such as cataract, glaucoma, diabetic retinopathy, and age-related macular degeneration have already been modelled in zebrafish. In addition, zebrafish have become an attractive model for pre-clinical drug toxicity testing and are now increasingly used by scientists worldwide for the discovery of novel treatment approaches. This review presents the advantages and uses of zebrafish for ophthalmological research.

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Section: Drug-related Ophthalmological Toxicity Assessment In Zebrafishmentioning

confidence: 99%

Section: Drug-related Ophthalmological Toxicity Assessment In Zebrafishmentioning

confidence: 99%

Section: Drug-related Ophthalmological Toxicity Assessment In Zebrafishmentioning

confidence: 99%

See 1 more Smart Citation

Zebrafish—on the move towards ophthalmological research

Chhetri

Jacobson

Gueven

2014

Eye

129

106

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“…Furthermore, non-invasive methods to screen for cardiac toxicity have been developed (Burns et al, 2005;Chan et al, 2009). A recently published review summarizes and describes the overall promising predictive capacity of zebrafish studies for drug toxicity (Eimon and Rubinstein, 2009). …”

Section: Danio Reriomentioning

confidence: 99%

Current standing and future prospects for the technologies proposed to transform toxicity testing in the 21st century

Vliet

2011

ALTEX

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“…1), dispensing them in up to 384-well plates is possible. 5 Keywords: toxicity testing, cell sorting, automated, multiwell plate, Zebrafish, danio rerio…”

Section: Introductionmentioning

confidence: 99%

Image-Based Fluidic Sorting System for Automated Zebrafish Egg Sorting into Multiwell Plates

Graf

Hötzel

Liebel

et al. 2011

JALA: Journal of the Association for Laboratory Automation

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T he global demand for the reduction of animal testing has led to the emergence of Zebrafish eggs/larvae as model organisms to replace current adult animal testing in, for example, toxicity testing. Because of the egg size (diameter 1.6 mm) and the relatively easy maintenance of Zebrafish farms the eggs also offer high-throughput screening (HTS). However, the current bottleneck for HTS is the cost-efficient placing of individual organisms into single wells of a multiwell plate (MWP). The system presented here is capable of storing, sorting, and placing individual organisms in a highly reproducible manner. In about 11 min a complete 96-MWP is filled, which corresponds to about 8 sec per egg. The survival rate of fertilized transgenic and wild-type eggs was comparable to the one of the control (control 6.7%, system 7.6%). Furthermore, it was also possible to place dechorionated eggs into individual wells. The results demonstrate that the cost efficient system works gentle and reliable enough to disburden scientists from the exhausting and monotonous job of placing single eggs into single wells, such that they can concentrate on the scientific aspects of their experiments and create results with a higher statistical relevance.

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The use ofin vivozebrafish assays in drug toxicity screening

Cited by 118 publications

References 45 publications

Zebrafish—on the move towards ophthalmological research

Zebrafish—on the move towards ophthalmological research

Current standing and future prospects for the technologies proposed to transform toxicity testing in the 21st century

Image-Based Fluidic Sorting System for Automated Zebrafish Egg Sorting into Multiwell Plates

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