The Use of Hydroponics in Abiotic Stress Tolerance Research

Shavrukov, Yuri; Genç, Yusuf; Hayes, J. F.

doi:10.5772/35206

Cited by 40 publications

(32 citation statements)

References 103 publications

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“…In this study, six barley varieties were chosen to explore the response patterns to longer-term salinity stress (150 mM NaCl for 4 weeks) in a hydroponics system, which is commonly used to assess plant salinity responses (Shavrukov et al, 2012). …”

Section: Discussionmentioning

confidence: 99%

“…After 2 days, seeds were transferred to a plant growth chamber (Fitotron, Weiss Gallenkamp, UK) for four days with the temperature set to constant 17°C. After germination, seedlings were transplanted into a hydroponic system as previously described (Shavrukov et al, 2012). Seedlings were distributed randomly to avoid systematic growth environment errors.…”

Section: Methodsmentioning

confidence: 99%

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A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

et al. 2017

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As integral parts of plant signaling networks, phytohormones are involved in the regulation of plant metabolism and growth under adverse environmental conditions, including salinity. Globally, salinity is one of the most severe abiotic stressors with an estimated 800 million hectares of arable land affected. Roots are the first plant organ to sense salinity in the soil, and are the initial site of sodium (Na+) exposure. However, the quantification of phytohormones in roots is challenging, as they are often present at extremely low levels compared to other plant tissues. To overcome this challenge, we developed a high-throughput LC-MS method to quantify ten endogenous phytohormones and their metabolites of diverse chemical classes in roots of barley. This method was validated in a salinity stress experiment with six barley varieties grown hydroponically with and without salinity. In addition to phytohormones, we quantified 52 polar primary metabolites, including some phytohormone precursors, using established GC-MS and LC-MS methods. Phytohormone and metabolite data were correlated with physiological measurements including biomass, plant size and chlorophyll content. Root and leaf elemental analysis was performed to determine Na+ exclusion and K+ retention ability in the studied barley varieties. We identified distinct phytohormone and metabolite signatures as a response to salinity stress in different barley varieties. Abscisic acid increased in the roots of all varieties under salinity stress, and elevated root salicylic acid levels were associated with an increase in leaf chlorophyll content. Furthermore, the landrace Sahara maintained better growth, had lower Na+ levels and maintained high levels of the salinity stress linked metabolite putrescine as well as the phytohormone metabolite cinnamic acid, which has been shown to increase putrescine concentrations in previous studies. This study highlights the importance of root phytohormones under salinity stress and the multi-variety analysis provides an important update to analytical methodology, and adds to the current knowledge of salinity stress responses in plants at the molecular level.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

et al. 2017

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“…After 2 days, seeds were transferred to a plant growth chamber (Fitotron, Weiss Gallenkamp, UK) with 16 h light and 8 h dark for 4 days with the temperature set to constant 17°C. After germination, seedlings were transplanted into a hydroponic system as previously described (Shavrukov et al, 2012). Seedlings were distributed randomly to avoid systematic errors.…”

Section: Growth Condition Salt Treatments and Biomass Measurementsmentioning

confidence: 99%

Insights Into Oxidized Lipid Modification in Barley Roots as an Adaptation Mechanism to Salinity Stress

et al. 2020

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“…The germinated seeds were directly sown on Sunshine R potting mix in pots or on Turface R artificial soil in 50mL falcon tubes and grown in growth room under 12: 12: light: dark regime. The composition of the liquid fertilizer used to nourish the plants was obtained from Shavrukov et al (2012). Photoperiod of the chamber was set up as 12 h light/12 h dark at 25 • C (EGC C6 Environmental Growth Chambers), providing the light of intensity 80 µmol m −2 s −1 photosynthetic photon flux.…”

Section: Growth Chamber Conditionsmentioning

confidence: 99%

Improved Yield and Photosynthate Partitioning in AVP1 Expressing Wheat (Triticum aestivum) Plants

et al. 2020

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A fundamental factor to improve crop productivity involves the optimization of reduced carbon translocation from source to sink tissues. Here, we present data consistent with the positive effect that the expression of the Arabidopsis thaliana H +-PPase (AVP1) has on reduced carbon partitioning and yield increases in wheat. Immunohistochemical localization of H +-PPases (TaVP) in spring wheat Bobwhite L. revealed the presence of this conserved enzyme in wheat vasculature and sink tissues. Of note, immunogold imaging showed a plasma membrane localization of TaVP in sieve element-companion cell complexes of Bobwhite source leaves. These data together with the distribution patterns of a fluorescent tracer and [U 14 C]-sucrose are consistent with an apoplasmic phloem-loading model in wheat. Interestingly, 14 C-labeling experiments provided evidence for enhanced carbon partitioning between shoots and roots, and between flag leaves and milk stage kernels in AVP1 expressing Bobwhite lines. In keeping, there is a significant yield improvement triggered by the expression of AVP1 in these lines. Green house and field grown transgenic wheat expressing AVP1 also produced higher grain yield and number of seeds per plant, and exhibited an increase in root biomass when compared to null segregants. Another agriculturally desirable phenotype showed by AVP1 Bobwhite plants is a robust establishment of seedlings.

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The Use of Hydroponics in Abiotic Stress Tolerance Research

Cited by 40 publications

References 103 publications

A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

Insights Into Oxidized Lipid Modification in Barley Roots as an Adaptation Mechanism to Salinity Stress

Improved Yield and Photosynthate Partitioning in AVP1 Expressing Wheat (Triticum aestivum) Plants

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