The use of human and bovine commercial media for oocyte maturation and embryo development in the domestic cat (Felis catus)

Abstract

The aim of this study was to examine the suitability of commercial media designed for humans and cattle for oocyte maturation and embryo culture in the domestic cat. In Exp. I, feline oocytes collected ex vivo were subjected to in vitro maturation in a laboratory-made culture medium (based on M199) or a commercial medium designed for cattle cells (BO-IVM ® ). In Exp. II, ICSI-derived feline embryos were cultured for 7 days in a commercial human (Continuous Single Culture ® ) or bovine (BO-EC ® ) cell medium. The rates of cleavage, morula and blastocyst formation were evaluated at 24 hr, 6 days and 7 days after ICSI, respectively, and compared between experimental groups. At the end of culture, embryos were assessed for viability and apoptotic changes. In Exp. I, no statistically significant difference in oocyte maturation outcome between laboratory-made (52.7%) and commercial media (58.9%) was observed. However, the use of a commercial medium prepared for use with bovine cells resulted in a significantly lower variance of the maturation rate. In Exp. II, no statistically significant differences between two commercial media were observed for cleavage (67.5% and 64.5%), morula (39.3% and 47.1%) and blastocyst rates (25.0% and 19.6%), as well as for the percentage of late apoptotic blastomeres. Morulae cultured in medium marketed for humans exhibited significantly more early apoptotic (43.2 ± 31.2% vs. 23.4 ± 23.2%) and necrotic (60.6 ± 47.6% vs. 29.4 ± 22.6%) blastomeres. In conclusion, both commercial media tested are suitable for in vitro oocyte maturation and embryo culture procedures in cats. It is remarkable that a culture medium designed for use in cattle for in vitro maturation of cat oocytes provides more reproducible results. K E Y W O R D Scat, commercial media, embryo culture, oocyte maturation

High efficiency of homemade culture medium supplemented with GDF9-β in human oocytes for rescue in vitro maturation

“…Another reason for this positive effect of GDF9 in homemade medium, but not commercial medium, may be the fact that commercial media are more complex than homemade media, which are made in-house. Commercial media, which are prepared with high quality control and standardized formulations, create an optimal environment for the immature oocytes, with no need for supplementation [ 32 ].…”

Mammalian Oocyte Analysis by MALDI MSI with Wet-Interface Matrix Deposition Technique

“…The oocytes were collected from female domestic cat ( Felis catus ) ovaries after a routine sterilization procedure performed at private local veterinary surgery and animal shelter (Krakow, Poland). The ovaries were transported to the laboratory in phosphate-buffered saline (PBS; Biomed, Lublin, Poland) supplemented with penicillin (100 IU/mL) and streptomycin (100 µg/mL) at 4 °C [ 20 ]. Cumulus-oocyte complexes (COCs) were recovered by slicing the ovaries in OPU-medium (Bioscience, Bickland Industrial Park, Falmouth, Cornwall, UK), washed in PBS and denuded of surrounding cumulus cells by mechanical pipetting, and kept in PBS at 4 °C up to 12 h. The oocytes were then: (i) washed three times with deionized water to remove the salts and buffer compounds (PBS-group) or (ii) fixed by incubating in 4% paraformaldehyde (PFA-group) dissolved in PBS for 15 min, followed by washing thrice in ultrapure water.…”

Fighting Like Cats and Dogs: Challenges in Domestic Carnivore Oocyte Development and Promises of Innovative Culture Systems

“…It is also worth mentioning that, both for IVM and IVC in the domestic cat, commercial media were experimented to increase reproducibility and repeatability and reduce the workload in the laboratory, and they were suitable for IVEP as well as lab-made media [68]. In this species, differences in the culture atmosphere during IVC were also investigated.…”