The use of host defense peptides in root canal therapy in rats

Lima, Stella M. F.; Freire, Mirna S.; Cantuária, Ana Paula de Castro; Martins, Danilo César Mota; Amorim, Ingrid Aquino; Dantas, Elaine M. G. L.; Farias, Jade Ormondes de; Castro, Márcio Botelho de; Silva, Jackson S.; Barriviera, Fernando A.; Barriviera, Maurício; Almeida, Jeeser Alves de; Uehara, Isadora Akemi; Silva, Marcelo José Barbosa; Oliveira, Ana Paula de Lima; Hancock, Robert E. W.; Franco, Octávio Luiz; Rezende, Taia Maria Berto

doi:10.1007/s00784-020-03684-9

Cited by 6 publications

(5 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we explored the use of the elastin-like recombinamer A200 as a purification tag for the production and isolation of Synoeca and CM4. These AMPs display strong antimicrobial activity against a wide range of bacteria, with no significant cytotoxicity against mammalian cells [26,27,[29][30][31][32], thus representing the opportunity to develop new antibiotic treatments to tackle antimicrobial resistance. The AMPs were fused to the N-terminus of the ELR A200, with an interspacing formic acid cleavage site, to enable the isolation and purification of the AMPs.…”

Section: Discussionmentioning

confidence: 99%

“…CM4 is a member of the cecropin family of peptides, with antibacterial, antifungal, and antitumor activities, while showing no toxicity towards mammalian cells [26,[28][29][30][31]. Synoeca is an AMP displaying strong activity against bacteria, including bacterial strains that are resistant to antibiotics, with no significant cytotoxicity against mammalian cell lines [27,32]. The DNA coding sequences of the AMPs were fused to the A200 gene, with an interspacing aspartate-proline (D-P) formic acid cleavage site for the isolation of AMPs from the ELR tag.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Production and Purification of Two Bioactive Antimicrobial Peptides Using a Two-Step Approach Involving an Elastin-Like Fusion Tag

et al. 2021

View full text Add to dashboard Cite

Antimicrobial resistance is an increasing global threat, demanding new therapeutic biomolecules against multidrug-resistant bacteria. Antimicrobial peptides (AMPs) are promising candidates for a new generation of antibiotics, but their potential application is still in its infancy, mostly due to limitations associated with large-scale production. The use of recombinant DNA technology for the production of AMPs fused with polymer tags presents the advantage of high-yield production and cost-efficient purification processes at high recovery rates. Owing to their unique properties, we explored the use of an elastin-like recombinamer (ELR) as a fusion partner for the production and isolation of two different AMPs (ABP-CM4 and Synoeca-MP), with an interspacing formic acid cleavage site. Recombinant AMP-ELR proteins were overproduced in Escherichia coli and efficiently purified by temperature cycles. The introduction of a formic acid cleavage site allowed the isolation of AMPs, resorting to a two-step methodology involving temperature cycles and a simple size-exclusion purification step. This simple and easy-to-implement purification method was demonstrated to result in high recovery rates of bioactive AMPs. The minimum inhibitory concentration (MIC) of the free AMPs was determined against seven different bacteria of clinical relevance (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and two Burkholderia cenocepacia strains), in accordance with the EUCAST/CLSI antimicrobial susceptibility testing standards. All the bacterial strains (except for Pseudomonas aeruginosa) were demonstrated to be susceptible to ABP-CM4, including a resistant Burkholderia cenocepacia clinical strain. As for Synoeca-MP, although it did not inhibit the growth of Pseudomonas aeruginosa or Klebsiella pneumoniae, it was demonstrated to be highly active against the remaining bacteria. The present work provides the basis for the development of an efficient and up-scalable biotechnological platform for the production and purification of active AMPs against clinically relevant bacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production and Purification of Two Bioactive Antimicrobial Peptides Using a Two-Step Approach Involving an Elastin-Like Fusion Tag

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Antimicrobial peptides, including HHC10 and HHC36, with comparable or even higher activity than many traditional antibiotics, were obtained (Cherkasov et al, 2009;Fjell et al, 2009). They investigated the potential of HHC10 to reduce periapical inflammation as a possible adjunct to pulp therapy (Lima et al, 2021). In later studies, Llamas-Gonzalez et al ( 2013) explored HHC10 antibacterial activity of Mycobacterium tuberculosis.…”

Section: Antimicrobial Peptides For Combating Bacterial Infectionsmentioning

confidence: 99%

Functional insights to the development of bioactive material for combating bacterial infections

Fan

Liu

Ren

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The emergence of antibiotic-resistant “superbugs” poses a serious threat to human health. Nanomaterials and cationic polymers have shown unprecedented advantages as effective antimicrobial therapies due to their flexibility and ability to interact with biological macromolecules. They can incorporate a variety of antimicrobial substances, achieving multifunctional effects without easily developing drug resistance. Herein, this article discusses recent advances in cationic polymers and nano-antibacterial materials, including material options, fabrication techniques, structural characteristics, and activity performance, with a focus on their fundamental active elements.

show abstract

“…The photosensitizer TPI-PN has good photodynamic activity, and the antimicrobial peptide HHC10 has been proved to have strong broad-spectrum antimicrobial activity in previous studies. [33][34][35][36] The hydrogel was formed by the Schiff base between the aldehyde group (ÀCHO) of OD and the amino group (ÀNH 2 ) of QCS. HHC10 and TPI-PN can also be combined into the hydrogel through the formation of imine bonds between structural amino groups and OD.…”

Section: Introductionmentioning

confidence: 99%

A peptide-based pH-sensitive antibacterial hydrogel for healing drug-resistant biofilm-infected diabetic wounds

Fan,

Xie,

Liu

et al. 2024

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

The use of host defense peptides in root canal therapy in rats

Cited by 6 publications

References 51 publications

Production and Purification of Two Bioactive Antimicrobial Peptides Using a Two-Step Approach Involving an Elastin-Like Fusion Tag

Production and Purification of Two Bioactive Antimicrobial Peptides Using a Two-Step Approach Involving an Elastin-Like Fusion Tag

Functional insights to the development of bioactive material for combating bacterial infections

A peptide-based pH-sensitive antibacterial hydrogel for healing drug-resistant biofilm-infected diabetic wounds

Contact Info

Product

Resources

About