The use of high-performance liquid chromatography with diode array detector for the determination of sulfide ions in human urine samples using pyrylium salts

Abstract

Hydrogen sulfide is a toxic gas involved in the regulation of some essential biological processes. A novel, precise, accurate and rapid method based on high-performance liquid chromatography with diode array detection for the determination of sulfide ions in human urine sample is proposed. The method involves the derivatization of sulfide with pyrylium salts -(2,4,6-triphenylpyrylium hydrogensulfate(VI) (L1) and 4-[p-(N,N-dimethylamino) phenyl]-2,6-diphenylpyrylium chlorate(VII) (LN1). The separation occurs on InfinityLab Poroshell 120 EC C 18 column using acetonitrile and phosphate buffer as a mobile phase. The detectors utilized a wavelength of 371 or 580 nm. The calibration curves were linear in the range of 2-150 μmol L −1 and 1-50 μmol L −1 for L1 and LN1 derivatives, respectively. The samples were found to be stable from sample collection to final analysis. The method was successfully applied to samples from apparently healthy volunteers.

Exfoliated Graphite: A Surface Renewed Electrode for Environmental Applications

“…In recent years, researchers have employed several methodologies and techniques to combat several issues, including water treatment, sensing of various toxic waste in water, and energy crisis to name a few (Dong et al, 2020;Bassi et al, 2021;Chen et al, 2021). Remarkable achievements have been reported in the literature in solving these problems using various analytical tools such as spectrophotometry (Al-Sulami et al, 2021), titrimetry (Basavaiah and Nagegowda, 2004), chemiluminescence (Sun et al, 2021), liquid chromatography (Alampanos and Samanidou, 2021), high-performance liquid chromatography (HPLC) (Zgagacz et al, 2020), and gas chromatography-mass spectrophotometry (GC-MS) (Xia et al, 2020). However, the shortcomings of these techniques include longer analysis time, tedious sample preparation, costly instrumentation, and non-portability of the instruments (Liang et al, 2019;Bagherinasab et al, 2020).…”