The Use of Herbicides in Biotech Oilseed Rape Cultivation and in Generation of Transgenic Homozygous Plants of Winter Oilseed Rape (Brassica napus L.)

Cegielska-Taras, Teresa; Pniewski, Tomasz

doi:10.5772/32601

Cited by 4 publications

(4 citation statements)

References 34 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Haploid structures are excellent materials for biotechnological manipulation and specific gene transfer, for example, by Agrobacterium tumefaciens-mediated delivery to haploid microspore embryos. A protocol for this has been developed in Tissue Cultures Laboratory of Department in Poznań (Cegielska-Taras & Pniewski, 2011;Cegielska-Taras et al, 2008). e high effectiveness of our method in microspore-derived embryo (MDE) regeneration and in vivo chromosome number duplication allowed us to participate in innovative projects in which the transformation of microspore-derived embryos with A. tumefaciens was used as a research tool.…”

Section: Dhs Of Winter Oilseed Rape (Brassica Napus L) In Plant Breed...mentioning

confidence: 99%

Doubled Haploids: Contributions of Poland’s Academies in Recognizing the Mechanism of Gametophyte Cell Reprogramming and Their Utilization in Breeding of Agricultural and Vegetable Species

et al. 2022

View full text Add to dashboard Cite

Diverse processes leading to doubled haploid (DH) plant production, such as microspore embryogenesis, gynogenesis, and distant hybridization followed by genome elimination, are based on the unique ability of plant cells to form haploid embryos without fertilization. All of these are possible because of various in vitro culture systems that enable the growth and development of tissues or single cells outside of the parental organism. The possibility of re-directing cell development from its original pathway to embryogenesis brings several benefits to many research areas, but the most important is the possibility of its implementation in breeding programs. This review summarizes the achievements of Polish research groups in studies of the mechanisms of haploid/DH embryo development and demonstrates the practical applications of these systems in basic studies and plant breeding. It shows the results of studies on economically important crops including barley ( Hordeum vulgare L.), oilseed rape ( Brassica napus L.), triticale (× Triticosecale Wittm.), oat ( Avena sativa L.), rye ( Secale cereale L.), sugar beet ( Beta vulgaris ssp. vulgaris L.), and some vegetable species, including carrot ( Daucus carota L.), onion ( Allium cepa L.), red beet ( Beta vulgaris L.), and members of the Brassicaceae.

show abstract

Section: Dhs Of Winter Oilseed Rape (Brassica Napus L) In Plant Breed...mentioning

confidence: 99%

Doubled Haploids: Contributions of Poland’s Academies in Recognizing the Mechanism of Gametophyte Cell Reprogramming and Their Utilization in Breeding of Agricultural and Vegetable Species

et al. 2022

View full text Add to dashboard Cite

show abstract

“…DHs obtained by tissue cultures of microspores and MDEs show uniformity, and as a complete homozygous, they can also be efficiently used in genetic transformation (Fukuoka et al, 1998;Nehlin et al, 2000;Takahata et al, 2005;Cegielska-Taras et al, 2008). The gene introduced into a haploid genome, which in this case was an MDE and subsequent chromosome doubling, gives rise to a homozygous transgenic oilseed rape (Cegielska-Taras et al, 2008b;Cegielska-Taras and Pniewski, 2011).…”

Section: Genetic Modification Of B Napusmentioning

confidence: 99%

“…The elaborated MDE transformation method of winter oilseed rape allows to obtain genetically modified plants (Cegielska-Taras et al, 2008b;Cegielska-Taras and Pniewski, 2011). The main advantage of this method is that as a result of chromosome duplication in the transformed haploid, the introduced trait can be evaluated in a single step, as a transgenic homozygote.…”

Section: Genetic Modification Of B Napusmentioning

confidence: 99%

Doubled haploids as a material for biotechnological manipulation and as a modern tool for breeding oilseed rape ( Brassica napus L.)

Cegielska-Taras¹,

Szała²,

Matuszczak³

et al. 2015

bta

Self Cite

View full text Add to dashboard Cite

Haploids and doubled haploids (DHs) have been produced in Brassica ssp. using anther or isolated microspore cultures. Since 1982, when Lichter developed a method of isolated microspores culture, this technique has been constantly improved and modified. Haploids and DH of Brassica napus have been extensively used in genetic studies, such as gene mapping, location of quantitative trait locus, marker / trait association studies, and genomics. Furthermore, the oilseed rape haploid induction technique can nowadays be efficiently combined with several other plant biotechnological techniques, enabling several novel breeding achievements, such as mutation breeding, the breeding of hybrid varieties, genetic transformation, and resynthesis of B. napus. The development and improvement of in vitro androgenesis of oilseed rape allowed DH populations to be obtained on a large scale. Today DH lines are used in the genetic analysis of quantitative traits and to analyze the impact of the environment on the yield and quality of the seeds. Currently, DH technology has become more efficient and hence can be widely applied in winter oilseed rape breeding. Additionally, DH technology is used to shorten the process of breeding new varieties. An open pollinated B. napus cv. Monolit (Plant Breeding Strzelce Ltd., Co.) is the first Polish winter oilseed rape variety obtained using DH technology. The breeding cycle of this variety is about four years shorter compared to a classical breeding program. The second Polish cultivar obtained using DH technology, cv. Brendy (Plant Breeding Smolice Ltd., Co.) was developed in a similarly short period. In the breeding of new oilseed rape hybrid varieties, homozygous restorer lines are also exploited. Our basic research used oilseed rape DH technology with a focus on improving the qualitative traits of oilseed rape and is presented here, based on the major researches conducted worldwide as well as on the results obtained at the Plant Breeding and Acclimati

show abstract

“…Rapeseed oil, free of anti-nutritional substances, has a higher dietary value than many other vegetable oils due to the low content of saturated fatty acids, high oleic acid content, favourable ratio of polyunsatured linoleic and linolenic acids, as well as presence of sterols and fat-soluble vitamins (Cegielska-Taras, Pniewski, 2011). In addition, the ratio of omega-6:omega-3 fatty acids in rapeseed oil is 2:1, which is beneficial for human health, as possessing antiinflammatory effects and protecting against heart diseases (Ion et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The influence of β-alanine derivative products on spring oilseed rape yield and oil quality

Kolosej

Jonuškienė

Venskutonis

et al. 2017

Zemdirbyste-Agriculture

View full text Add to dashboard Cite

Please use the following format when citing the article: Kolosej R., Jonuškienė I., Venskutonis P. R., Kazernavičiūtė R., Brazienė Z., Jakienė E., Kvederavičiūtė K., Kanopka A., Vilys L., Mickevičius V. 2017. The influence of β-alanine derivative products on spring oilseed rape yield and oil quality. Field experiments showed that 125 mg l -1 NPNDT solution had the greatest effect on plant growth. In this treatment, the number of secondary branches increased by 54%, the rapeseed yield by 23% compared with the control treatment. The highest seed number per silique (24.5 seeds, or 7.5% higher than in the control) was determined in the treatment where oilseed rape seedlings had been sprayed with 75 mg l -1 NPNDT solution. The highest oil and protein contents in rapeseed were determined in the 75 mg l -1 NPNDT treatment. Compared with the control, a statistically significant increase in rapeseed oil amounted to 137.9 kg ha -1 or 17% and protein content to 6.2 g 100 g -1 or 31%. The increase in rapeseed yield was statistically significant (P < 0.05) in all treatments. The increase of oil content was positively correlated with the seed yield increase (P < 0.05) when oilseed rape was sprayed with the 50 mg l -1 concentration of NPNDT solution. The NPNDT had a positive effect on oil quality. It increased protein and flavonoid contents, reduced ash content in rapeseed and increased DPPH radical scavenging in rapeseed extract.

show abstract

The Use of Herbicides in Biotech Oilseed Rape Cultivation and in Generation of Transgenic Homozygous Plants of Winter Oilseed Rape (Brassica napus L.)

Cited by 4 publications

References 34 publications

Doubled Haploids: Contributions of Poland’s Academies in Recognizing the Mechanism of Gametophyte Cell Reprogramming and Their Utilization in Breeding of Agricultural and Vegetable Species

Doubled Haploids: Contributions of Poland’s Academies in Recognizing the Mechanism of Gametophyte Cell Reprogramming and Their Utilization in Breeding of Agricultural and Vegetable Species

Doubled haploids as a material for biotechnological manipulation and as a modern tool for breeding oilseed rape ( Brassica napus L.)

The influence of β-alanine derivative products on spring oilseed rape yield and oil quality

Contact Info

Product

Resources

About