Herbicides - Mechanisms and Mode of Action 2011
DOI: 10.5772/32601
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The Use of Herbicides in Biotech Oilseed Rape Cultivation and in Generation of Transgenic Homozygous Plants of Winter Oilseed Rape (Brassica napus L.)

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Cited by 4 publications
(4 citation statements)
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“…Haploid structures are excellent materials for biotechnological manipulation and specific gene transfer, for example, by Agrobacterium tumefaciens-mediated delivery to haploid microspore embryos. A protocol for this has been developed in Tissue Cultures Laboratory of Department in Poznań (Cegielska-Taras & Pniewski, 2011;Cegielska-Taras et al, 2008). e high effectiveness of our method in microspore-derived embryo (MDE) regeneration and in vivo chromosome number duplication allowed us to participate in innovative projects in which the transformation of microspore-derived embryos with A. tumefaciens was used as a research tool.…”
Section: Dhs Of Winter Oilseed Rape (Brassica Napus L) In Plant Breed...mentioning
confidence: 99%
“…Haploid structures are excellent materials for biotechnological manipulation and specific gene transfer, for example, by Agrobacterium tumefaciens-mediated delivery to haploid microspore embryos. A protocol for this has been developed in Tissue Cultures Laboratory of Department in Poznań (Cegielska-Taras & Pniewski, 2011;Cegielska-Taras et al, 2008). e high effectiveness of our method in microspore-derived embryo (MDE) regeneration and in vivo chromosome number duplication allowed us to participate in innovative projects in which the transformation of microspore-derived embryos with A. tumefaciens was used as a research tool.…”
Section: Dhs Of Winter Oilseed Rape (Brassica Napus L) In Plant Breed...mentioning
confidence: 99%
“…DHs obtained by tissue cultures of microspores and MDEs show uniformity, and as a complete homozygous, they can also be efficiently used in genetic transformation (Fukuoka et al, 1998;Nehlin et al, 2000;Takahata et al, 2005;Cegielska-Taras et al, 2008). The gene introduced into a haploid genome, which in this case was an MDE and subsequent chromosome doubling, gives rise to a homozygous transgenic oilseed rape (Cegielska-Taras et al, 2008b;Cegielska-Taras and Pniewski, 2011).…”
Section: Genetic Modification Of B Napusmentioning
confidence: 99%
“…The elaborated MDE transformation method of winter oilseed rape allows to obtain genetically modified plants (Cegielska-Taras et al, 2008b;Cegielska-Taras and Pniewski, 2011). The main advantage of this method is that as a result of chromosome duplication in the transformed haploid, the introduced trait can be evaluated in a single step, as a transgenic homozygote.…”
Section: Genetic Modification Of B Napusmentioning
confidence: 99%
“…Rapeseed oil, free of anti-nutritional substances, has a higher dietary value than many other vegetable oils due to the low content of saturated fatty acids, high oleic acid content, favourable ratio of polyunsatured linoleic and linolenic acids, as well as presence of sterols and fat-soluble vitamins (Cegielska-Taras, Pniewski, 2011). In addition, the ratio of omega-6:omega-3 fatty acids in rapeseed oil is 2:1, which is beneficial for human health, as possessing antiinflammatory effects and protecting against heart diseases (Ion et al, 2010).…”
Section: Introductionmentioning
confidence: 99%