The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response

Hartwig, Daiane Drawanz; Bacelo, Kátia Leston; Oliveira, Thaís Larré; Schuch, Rodrigo Andrade; Seixas, Fabiana Kömmling; Collares, Tiago; Rodrigues, Oscar E. D.; Hartleben, Cláudia Pinho; Dellagostin, Odir A.

doi:10.1590/0074-02760140276

Cited by 15 publications

(10 citation statements)

References 30 publications

(37 reference statements)

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“…Additionally, several studies have shown that functionalised CNTs do not cause cytotoxic effects (10,11,28,29) as observed in this work. Amino-functionalised NPs were found to interact with negatively charged DNA and the cell membrane, and through this interaction, these successfully delivered the pTARGET/lemA plasmid into mammalian cells without any cytotoxicity.…”

Section: Discussionsupporting

confidence: 79%

“…Freund's adjuvant, (7) aluminum hydroxide, (5) Ad-daVax ™ , (6) CpG oligodeoxynucleotides (or CpG ODN), xanthan gum, (8) mannosylated form, (9) and NPs. (10,11) NPs seemed to be potential antigen carriers for the development of new-generation vaccines, and combined nanovaccines have been known to induce more intense and long-lasting antigen-specific immune responses. NPs can induce a robust innate immunity response by causing abundant initial antigen exposure and long-term immunity through the sustained release of antigens via two types of activation modes: dendritic cell (DC) and T-cell differentiation.…”

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confidence: 99%

“…NPs can induce a robust innate immunity response by causing abundant initial antigen exposure and long-term immunity through the sustained release of antigens via two types of activation modes: dendritic cell (DC) and T-cell differentiation. (12,13,14) In previous studies, we demonstrated that subunit vaccines containing recombinant proteins, LipL32 (10) and LigAni, (11) with halloysite nanotubes (HNTs) and carboxylated multi-walled carbon nanotubes (COOH-MWCNTs) as carriers induced a strong IgG immune response in Golden Syrian hamsters.…”

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confidence: 99%

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DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection

Oliveira

Bacelo

Forster

et al. 2020

Mem. Inst. Oswaldo Cruz

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BACKGROUND Nanoparticles (NPs) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the DNA vaccination strategy against leptospirosis. OBJECTIVES We evaluated the efficiency of halloysite clay nanotubes (HNTs) and amine-functionalised multi-walled carbon nanotubes (NH 2-MWCNTs) in facilitating recombinant LemA antigen (rLemA) expression and protecting Golden Syrian hamsters (Mesocricetus auratus) against Leptospira interrogans lethal infection. METHODS An indirect immunofluorescent technique was used to investigate the potency of HNTs and NH 2-MWCNTs in enhancing the transfection and expression efficiency of the DNA vaccine in Chinese hamster ovary (CHO) cells. Hamsters were immunised with two doses of vaccines HNT-pTARGET/lemA, NH 2-MWCNTs-pTARGET/lemA, pTARGET/lemA, and empty pTARGET (control), and the efficacy was determined in terms of humoral immune response and protection against a lethal challenge. FINDINGS rLemA DNA vaccines carried by NPs were able to transfect CHO cells effectively, inducing IgG immune response in hamsters (p < 0.05), and did not exhibit cytotoxic effects. Furthermore, 83.3% of the hamsters immunised with NH 2-MWCNTs-pTARGET/lemA were protected against the lethal challenge (p < 0.01), and 66.7% of hamsters immunised with HNT-pTARGET/ lemA survived (p < 0.05). MAIN CONCLUSIONS NH 2-MWCNTs and HNTs can act as antigen carriers for mammalian cells and are suitable for DNA nanovaccine delivery.

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Section: Discussionsupporting

confidence: 79%

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confidence: 99%

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confidence: 99%

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DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection

Oliveira

Bacelo

Forster

et al. 2020

Mem. Inst. Oswaldo Cruz

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“…Immunisation with a surface protein of Anaplasma marginale , rMSP1a, associated with MWCNTs, significantly induced high levels of anti-MSP1a IgG (Silvestre et al 2014). Recently, the association of halloysite and COOH-MWCNTs with LipL32 antigen from L. interrogans was shown to enhance IgG response at higher levels than that obtained through Alhydrogel; however, none of the animals were protected against challenge (Hartwig et al 2015). …”

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confidence: 99%

Immune response in hamsters immunised with a recombinant fragment of LigA from Leptospira interrogans, associated with carrier molecules

Oliveira

Bacelo

Schuch

et al. 2016

Mem. Inst. Oswaldo Cruz

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Immunisation with the C-terminal region of leptospiral immunoglobulin-like A protein (LigANI) has shown promising results against leptospirosis. We evaluated the humoral immune response and protection induced by LigANI associated with carboxyl multi-walled carbon nanotubes (COOH-MWCNTs), CpG oligodeoxynucleotides (CpG ODNs), or Alhydrogel. Animals immunised with CpG ODNs were unable to develop a humoral immune response, whereas immunisation with LigANI and COOH-MWCNTs produced a high level of IgG antibodies, similar to that with LigANI and Alhydrogel, but it was not protective. The use of carbon nanotubes as an adjuvant in subunit vaccines against leptospirosis is a novel approach for improving specific IgG production.

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“…Immunization using the HNTs and COOH-MWCNTs increased to a remarkable extent the rLipL32-specific IgG antibody titres of golden Syrian hamsters with leptospirosis disease (Hartwig et al, 2015). Gene therapy is facing problems with use of viral vectors; in recent years, therefore, new non-viral gene carriers have attracted much attention.…”

Section: H N Ts U S E D I N T H E D E L I V E Ry O F Vac C I N E S a mentioning

confidence: 99%

Halloysite nanotubes as a new drug-delivery system: a review

et al. 2016

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New drug-delivery systems have remained a challenge for pharmaceutical scientists due to the use of expensive polymers and the low loading capacity of prepared nanoparticles. There is pressure to develop formulations that contain not only cheaper materials but also have controlled-release properties. Halloysite nanotubes (HNTs) are a naturally occurring clay mineral similar to kaolin, possessing a special particle shape in the form of an ultramicroscopic multilayered hollow cylinder. Its uses encompass a wide range in anticancer therapy, sustained- and controlled-release drug-delivery systems, cosmetics, delivery of proteins, vaccines and genes. These advantages are due to its biocompatibility, significant mechanical strength and natural availability. The surfaces of the tubules can be modified by coating different polymers for application in the drug-delivery system. This review is focused on the various aspects of HNTs such as structure, properties, loading methods, applications and characterizations.

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The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response

Cited by 15 publications

References 30 publications

DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection

DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters against Leptospira lethal infection

Immune response in hamsters immunised with a recombinant fragment of LigA from Leptospira interrogans, associated with carrier molecules

Halloysite nanotubes as a new drug-delivery system: a review

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