The use of graft copolymers as enzyme supports

Abdel-Hay, F. I.; Guthrie, James T.; Morrish, Courtney; Beddows, C. G.

doi:10.1007/bf00256276

Cited by 11 publications

(1 citation statement)

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“…The immobilization of enzymes on insoluble supporting materials has been carried out extensively by many re searchers (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) . Beddows and his coworkers (7)(8)(9)(10)(11)(12) used grafted polymers as supporters of the enzymes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Molecular Weight and Polymer Composition on Coupling Reactions of Lipase and Polyacrylic Acid or Acrylic Acid/Acrylamide Copolymers.

et al. 1996

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Section: Introductionmentioning

confidence: 99%

Effects of Molecular Weight and Polymer Composition on Coupling Reactions of Lipase and Polyacrylic Acid or Acrylic Acid/Acrylamide Copolymers.

et al. 1996

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Photosensitized grafting of acrylamide and hydroxyethyl methacrylate onto cellulose

Silva

Gil

Lapa

et al. 1987

J of Applied Polymer Sci

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SynopsisThe photoinitiated copolymerization of acrylamide and hydroxyethyl methacrylate onto cellulose substrates, from acetone/water solutions, was studied using isopropyl thioxanthone as the photoinitiator. The graft copolymerization was carried out over a range of monomer and photoinitiator concentrations. Grafting is shown to be dependent on the nature of the monomer and the concentration of monomer and photoinitiator. In the case of acrylamide, it was found that there is an optimum concentration of the photoinitiator for grafting to occur effectively. The kinetics of graft copolymerization were studied in some detail and the results are considered.

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Studies on the physical properties of polyethylene‐g‐acrylic acid to immobilizing glucose oxidase

Hsiue¹,

Wang²

1990

J of Applied Polymer Sci

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SynopsisA new type of polymer support for immobilizing glucose oxidase was investigated. The starting copolymer was acrylic-acid-grafted polyethylene ( PE-g-AA) . The copolymer was prepared with gamma ray irradiation induced grafting under controlled conditions utilizing post-irradiation procedures. A number of factors were examined. They were: (1) the effect of the hydrophilic/hydrophobic nature of the support on the enzyme membrane activity; ( 2 ) the Michaelis constant (k,,,); (3) the thermal stability, the rate constant for the thermal denaturation, and the activation energies for the denaturation of immobilized and soluble enzyme; and ( 4 ) the physical properties of the support affecting the immobilized enzyme activity. An attempt was made to immobilize the enzymeutilizing carhodiimide (CMC) as the coupling agent. This immobilization method resolves in a high enzyme activity. The hydrophilicity of the support and the activity of the enzyme membrane were proportional to the degree of grafting. When the temperature was higher than 60"C, the immobilized glucose oxidase was less sensitive to thermal inactivation than the native enzyme. Various polyethylene was studied; the LLDPE was preferred. The type of polyethylene, the thickness, kind of grafting monomers, and the degree of grafting would influence the activity of the enzyme membrane.

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The use of graft copolymers as enzyme supports

Cited by 11 publications

References 0 publications

Effects of Molecular Weight and Polymer Composition on Coupling Reactions of Lipase and Polyacrylic Acid or Acrylic Acid/Acrylamide Copolymers.

Effects of Molecular Weight and Polymer Composition on Coupling Reactions of Lipase and Polyacrylic Acid or Acrylic Acid/Acrylamide Copolymers.

Photosensitized grafting of acrylamide and hydroxyethyl methacrylate onto cellulose

Studies on the physical properties of polyethylene‐g‐acrylic acid to immobilizing glucose oxidase

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