The use of glass wool as an attachment surface for studying phenotypic changes in Pseudomonas aeruginosa biofilms by two-dimensional gel electrophoresis

Steyn, Bridgitta; Oosthuizen, Marinda C.; MacDonald, Raynard; Théron, J; Brözel, Volker S.

doi:10.1002/1615-9861(200107)1:7<871::aid-prot871>3.3.co;2-u

Cited by 21 publications

(27 citation statements)

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“…Thus, DNA arrays provide a sensitive but transient snapshot of gene expression and proteomics provide a partial sight of protein expression directly correlated with phenotype. While changes in protein expression during biofilm development have been investigated for a large panel of bacterial species (Arevalo-Ferro et al 2005;Dykes et al 2003;Oosthuizen et al 2001Oosthuizen et al , 2002Sauer et al 2001Sauer et al , 2002Steyn et al 2001;Svensä ter et al 2001;Tré moulet et al 2002a;Vilain et al 2004 a, b, c), a limited number of studies have been conducted to determine the impact of the sessile mode of life on E. coli protein expression (Perrot et al 2000;Perrot et al 2001;Tré moulet et al 2002b). These studies have revealed some alterations in the bacterial protein profiles ranging from 3% to more than 50% of the detected protein spots, which gives evidence of significant physiological differences between planktonic and sessile bacteria.…”

Section: Introductionmentioning

confidence: 99%

Protein expression in Escherichia coli S17-1 biofilms: impact of indole

Collet

Vilain

Cosette

et al. 2006

Antonie van Leeuwenhoek

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Bacteria undergo significant changes during adherence to surfaces and biofilm development. Cell-to-cell signalling molecules are known to be involved in these phenotypic adaptations to the sessile mode of life. We demonstrated previously that indole can act as an extracellular signal to regulate biofilm formation in E. coli. To identify proteins over- or under-expressed in response to E. coli biofilm formation and indole signalling, we compared the proteomes of the E. coli S17-1 wild-type and 3714 (S17-1 tnaA::Tn5) tryptophanase-negative mutant cells (which don't produce indole) grown as suspensions or biofilms in the presence or absence of exogenous indole. From computer-assisted image analysis, 407 spots were discriminated on two-dimensional electropherograms. Principal component analysis (PCA) of the electropherograms did not discriminate between the proteomes of the wild-type and mutant cells grown as suspensions indicating that indole has a limited impact onto protein expression of planktonic cells. The first principal component extracted by PCA, after standardization of the observations, opposed planktonic and biofilm cells confirming the existence of changes in protein expression during E. coli biofilm formation. Among proteins over- or under-expressed by both sessile wild-type and mutant cells, we identified metabolic enzymes, transporters, proteins involved in the translation and transcription machinery, stress response and regulation, and signalling proteins. The wild-type and mutant strains grown as biofilms in the presence of indole were discriminated by the second component. The role of some proteins whose expression was altered in biofilm bacteria compared to suspended counterparts is discussed.

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Section: Introductionmentioning

confidence: 99%

Protein expression in Escherichia coli S17-1 biofilms: impact of indole

Collet

Vilain

Cosette

et al. 2006

Antonie van Leeuwenhoek

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“…Protein [14][15][16] and LPS [2] profiles also undergo changes in a bacterial cell when it switches from planktonic to sessile form of growth.…”

Section: Introductionmentioning

confidence: 99%

Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish

Asha¹,

Nayak²,

Shankar³

et al. 2004

Fish & Shellfish Immunology

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“…The cells from a glass flask, a substratum that does not support biofilm formation by IHE 3034-Rif, were used as a reference, as an inoculum, and were defined as 'inoculate' cells; the cells in the glass flasks at the end of the incubation periods were termed 'planktonic' cells. The non-attached cells removed from the PVC wells after biofilm growth were defined as 'surface-influenced planktonic (SIP)' cells, according to Steyn et al (2001), and the surface-attached cells harvested from the walls of the PVC wells were termed 'biofilm' cells (see Methods). After 24 or 48 h of static growth in M63 medium at 20 u C, the mean Mat expression levels of the entire cell populations were quantified by whole-cell ELISA.…”

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Mat fimbriae promote biofilm formation by meningitis-associated Escherichia coli

et al. 2010

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The mat (or ecp) fimbrial operon is ubiquitous and conserved in Escherichia coli, but its functions remain poorly described. In routine growth media newborn meningitis isolates of E. coli express the meningitis-associated and temperature-regulated (Mat) fimbria, also termed E. coli common pilus (ECP), at 20 6C, and here we show that the six-gene (matABCDEF)-encoded Mat fimbria is needed for temperature-dependent biofilm formation on abiotic surfaces. The matBCDEF deletion mutant of meningitis E. coli IHE 3034 was defective in an early stage of biofilm development and consequently unable to establish a detectable biofilm, contrasting with IHE 3034 derivatives deleted for flagella, type 1 fimbriae or S-fimbriae, which retained the wild-type biofilm phenotype. Furthermore, induced production of Mat fimbriae from expression plasmids enabled biofilm-deficient E. coli K-12 cells to form biofilm at 20 6C. No biofilm was detected with IHE 3034 or MG1655 strains grown at 37 6C. The surface expression of Mat fimbriae and the frequency of Mat-positive cells in the IHE 3034 population from 20 6C were high and remained unaltered during the transition from planktonic to biofilm growth and within the matured biofilm community. Considering the prevalence of the highly conserved mat locus in E. coli genomes, we hypothesize that Mat fimbria-mediated biofilm formation is an ancestral characteristic of E. coli. INTRODUCTIONEscherichia coli is a multifaceted bacterium that colonizes the mammalian intestine as a harmless commensal but also causes a repertoire of intestinal and extraintestinal infectious diseases (Dobrindt, 2005) and survives in the environment (Savageau, 1983). E. coli from the mother and/or the surrounding environment are generally among the earliest colonizers in the oxygenous neonatal gut. During the first months of life a succession of bacterial populations progresses to a complex, more stable and adult-like microbial community dominated by strict anaerobes (Adlerberth & Wold, 2009). However, some E. coli strains are able to persist as a member of the normal microbiota and constitute a major portion of the facultative intestinal flora on adult mucosal surfaces. E. coli also frequently colonizes anatomical locations outside the gastrointestinal tract, e.g. vagina and urinary bladder (Obata-Yasuoka et al., 2002; Rosen et al., 2007). The population structure of E. coli is largely clonal (Ochman & Selander, 1984;Selander et al., 1986), and isolates are frequently categorized into distinct pathogroups according to specific combinations of phenotypic traits. Based on multilocus enzyme electrophoresis, E. coli strains fall into four main phylogenetic groups (designated A, B1, B2 and D), each containing varying proportions of different pathogroups and non-pathogens. E. coli strains resident in childhood and adult microbiota commonly include pathogenic variants (Sarff et al., 1975;Siitonen, 1992), predominantly belonging to the virulenceassociated phylogenetic group B2 (Nowrouzian et al., 2005; Obata-Yasuoka et al., 200...

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The use of glass wool as an attachment surface for studying phenotypic changes in Pseudomonas aeruginosa biofilms by two-dimensional gel electrophoresis

Cited by 21 publications

References 0 publications

Protein expression in Escherichia coli S17-1 biofilms: impact of indole

Protein expression in Escherichia coli S17-1 biofilms: impact of indole

Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish

Mat fimbriae promote biofilm formation by meningitis-associated Escherichia coli

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