The use of gas-liquid chromatography in the analysis of neutral monosaccharides in hydrolysates of gastric mucopolysaccharides

Abstract

1. Conditions are described for the separation and estimation of the neutral monosaccharides obtained on acidic hydrolysis of human gastric mucopolysaccharides. 2. The technique involves the formation of the trimethylsilyl derivatives of the sugars and the analysis of these by gas-liquid chromatography. 3. The monosaccharides estimated in gastric mucopolysaccharides by this technique were l-fucose, d-mannose, d-galactose and d-glucose. 4. The analytical values for glucose and fucose obtained by this method agreed well with values obtained by the glucose oxidase and thioglycollic acid methods respectively. 5. Evidence is presented which clearly indicates that gas-liquid chromatography is a faster, more sensitive and more convenient technique for the measurement of these compounds than any other in use at present.

Mucinous Secretion of Totally Isolated Porcine Stomach Perfused with Homologous Blood

“…This was done previously by other investigators [1,[17][18][19][20][21][22], Nor will we try to compare our results with those obtained in in vivo con ditions by different methods and in different species [1-3, 15, 25-27], In our discussion we will essentially refer to the studies of Schrager and Oates, which inspired our work and which supplied us with the metho dology.…”

“…The techniques employed here for analysis of components of gastric mucus were those described by Schrager |18, 19| and Schrager and Oates [17,[20][21][22], We determined carbohydrate components D-galactose, D-mannose, L-fucose, D-glucosamine and D-galactosaniine [17,[19][20][21][22], We also analyz.ed these samples for total sulfate and total sugars [18,21] and for amino acids.…”

The Composition and some Structural Features of the Principal Gastric Glycoprotein

“…The successful application of gas-liquid chrom atography to de term ine each of the carbohydrate components of the hydrolysates of gastric secretion facilitated the study of the carbohydrate compo sition of a large num ber of gastric aspirations [7,8]. The data ob tained showed a constant quantitative relationship between these components and suggested th a t the gastric macromolecular carbo hydrate-containing fractions have a common basic core and th at…”

Gas‐chromatographische Untersuchungen an Uronsäuren und Uronsäurederivaten

“…Wahrend beim heterogenen Umsatz von kristallisierten reduzierenden Zuckern mit dem Reagenziengemisch im Gas-Chromatogramm nur je ein Pik fur die im Kristall vorliegende Halbacetalform auftritt, erhalt man aus Losungen eines mutarotierenden Zuckers in der Regel mehrere Pike [2], was die Analyse eines Mehrkomponentengemisches erschwert oder sogar verunmoglicht. Bei den Aldosen lasst sich die Schwierigkeit durch Reduktion [l] [3] oder Oxydation [4] der Aldehydgruppe umgehen.…”