The use of FTIR microscopy for the evaluation of anti-bacterial agents activity

Huleihel, Mahmoud; Pavlov, Valentina; Erukhimovitch, Vitaly

doi:10.1016/j.jphotobiol.2009.03.009

Cited by 32 publications

(23 citation statements)

References 23 publications

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“…13,14) Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have antibacterial, anticancer, anti-inflammatory and immunomodulatory properties; the anti-inflammatory effects have been known for a long time. [15][16][17][18][19] However, its anti-inflammatory effect on hypertrophic adipocytes is not well understood. We have reported that CAPE has suppression effect on 3T3-L1 differentiation to adipocytes and production of adipocytokines during adipogenesis.…”

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confidence: 99%

Caffeic Acid Phenethyl Ester Suppresses the Production of Pro-inflammatory Cytokines in Hypertrophic Adipocytes through Lipopolysaccharide-Stimulated Macrophages

Juman

Yasui

Ikeda

et al. 2012

Biological & Pharmaceutical Bulletin

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Obesity is a condition in which excess body fat accumulates due to lipids producing adipocytes and an increased number of differentiated mature cells. Recently, new findings have shown that macrophages infiltrate into adipose tissues and produce various pro-inflammatory cytokines in obese subjects. The inflammatory changes induced by the cross-talk between adipocytes and macrophages are critical for the pathophysiology of obesity and thus of metabolic syndrome. Caffeic acid phenethyl ester (CAPE) is known to have many functions, including antibacterial, anticancer and anti-inflammatory properties, but there is no evidence of its effect on the inflammatory responses in hypertrophic adipocytes through stimulation by macrophages. We investigated the effect of CAPE on macrophages and hypertrophic adipocytes in this study. CAPE significantly suppressed the levels of lipopolysaccharide (LPS)-induced interleukin (IL)-1-beta, tumor necrosis factor (TNF)-alpha and monocyte chemoattractant protein (MCP)-1 from a macrophage cell line, RAW264.7. Supernatants of stimulated RAW264.7 macrophages drastically increased mRNA levels of pro-inflammatory cytokines such as IL-6, MCP-1 and TNF-alpha in 3T3-L1 hypertrophic adipocytes. CAPE also significantly and dose-dependently reduced the gene expression of these cytokines. Our findings indicate that CAPE has inhibitory effects on the production of pro-inflammatory cytokines from LPS-stimulated RAW264.7 macrophages. In addition, CAPE suppressed gene expressions of cytokines under inflammatory conditions of hypertrophic adipocytes, suggesting that it may have the potential to suppress inflammation by macrophage infiltration into adipose tissue in obese patients.Key words caffeic acid phenethyl ester; hypertrophic adipocyte; macrophage; pro-inflammatory cytokine Obesity is a major risk factor for metabolic syndrome, which includes a number of disorders, such as hypertension, heart diseases and diabetes.1,2) Obesity is a condition in which excess body fat accumulates due to lipids producing hypertrophic adipocytes and an increased number of differentiated mature cells. This process is regulated by genetic and environmental factors, such as nutrient intake.3,4) It is known that adipocytes in obese individuals show cell hypertrophy and chronic inflammation. The inflammatory changes induced by the cross-talk between adipocytes and macrophages are critical for the pathophysiology of obesity and thus of metabolic syndrome. 5,6) Recent studies have demonstrated that obese adipose tissue is characterized by increased infiltration of macrophages, 7,8) which produce various inflammatory proteins. The inflammation process is associated with the activation of macrophages and the release of pro-inflammatory cytokines, such as interleukin (IL)-1-beta, tumor necrosis factor (TNF)-alpha, monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-6. 9,10) MCP-1, known to play a pivotal role in macrophage trafficking and activation, is produced by macrophages and adipocytes. Several reports suggest t...

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Caffeic Acid Phenethyl Ester Suppresses the Production of Pro-inflammatory Cytokines in Hypertrophic Adipocytes through Lipopolysaccharide-Stimulated Macrophages

Juman

Yasui

Ikeda

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Huleihel et al investigated the effect of the commonly applied b-lactam antibiotic ampicillin (100 mg l À1 ) on Gram-negative bacteria (E. coli, Serratia marcescens, P. aeruginosa, H. influenza, and Salmonella enteridis) and also on Gram-positive bacteria (S. aureus, Staphylococcus olysgalacticae, Staphylococcus mitis, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, and Bacillus thuringiensis) by means of FTIR spectroscopy [66]. In contrast to SERS, which provides chemical information only about the bacterial cell wall, FTIR spectroscopy gives information about all the bacteria cell components.…”

Section: Effect Of Antibiotics On Microorganismsmentioning

confidence: 99%

Identification and Characterization of Microorganisms by Vibrational Spectroscopy

Stöckel

Walter

Boßecker

et al. 2012

Handbook of Biophotonics

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Vibrational spectroscopic methods such as infrared absorption and Raman spectroscopy achieve a so‐called spectroscopic fingerprint by probing microorganisms. This spectral contribution is related to the total chemical components inside the cell. It is therefore possible to identify microorganisms by determining whether their spectroscopic fingerprint could have come from the same kind of bacteria whose fingerprint has already been measured and stored in a database. Since spectral databases are normally very large, sophisticated chemometric analyses are required for bacterial identification, hence a summary of commonly used chemometric methods for data preprocessing and evaluation is included in this chapter. Typical sample preparation techniques are also described before summarizing the application of vibrational spectroscopic methods in the fields of biofilm analysis, epidemiological studies, antibiotic effects on bacteria, and the detection of food‐borne pathogens.

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“…In addition, many caffeic acid analogues also had great potential as anti-fungal and anti-bacterial agents [11]. Huleihel M et al found that the caffeic acid phenethyl ester could effect some biomarker in bacteira by FTIR microspectroscopy [12]. Caffeic acid also could present antibacterial properties by pH dependent [13].…”

Section: Introductionmentioning

confidence: 99%

Anti-tyrosinase kinetics and antibacterial process of caffeic acid N-nonyl ester in Chinese Olive (Canarium album) postharvest

Jia

Zheng

Feng

et al. 2016

International Journal of Biological Macromolecules

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Enzymatic browning and bacterial putrefaction are mainly responsible for quality losses of Chinese Olive (Canarium album) postharvest and lead to very short shelf life on average. Screening anti-browning and anti-bacterial agents is important for preservation of Chinese Olive. Caffeic acid N-nonyl ester (C-9) and caffeic acid N- Heptyl ester (C-7) was synthesized as inhibitors of tyrosinase, which is a key enzyme in browning process. The compound of C-9 could inhibit the activity of tyrosinase strongly and its IC50 value was determined to be 37.5μM, while the compound of C-7 had no inhibitory ability. Kinetic analyses showed that compound of C-9 has been a reversible inhibitory mechanism below 50μM and been irreversible mechanisms above 50μM. For the reversible inhibitory mechanism, the values of inhibitory constants (KI and KIS) were determined to be 24.6 and 37.4μM, respectively. The results of Chinese Olive fruit postharvest showed that the compound of C-9 could effectively anti-browning and anti-bacterial putrefaction. In addition, this compound had strong antibacterial activities against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella. Therefore, C-9 could be a potential anti-browning and anti-bacterial reagent.

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The use of FTIR microscopy for the evaluation of anti-bacterial agents activity

Cited by 32 publications

References 23 publications

Caffeic Acid Phenethyl Ester Suppresses the Production of Pro-inflammatory Cytokines in Hypertrophic Adipocytes through Lipopolysaccharide-Stimulated Macrophages

Caffeic Acid Phenethyl Ester Suppresses the Production of Pro-inflammatory Cytokines in Hypertrophic Adipocytes through Lipopolysaccharide-Stimulated Macrophages

Identification and Characterization of Microorganisms by Vibrational Spectroscopy

Anti-tyrosinase kinetics and antibacterial process of caffeic acid N-nonyl ester in Chinese Olive (Canarium album) postharvest

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