The use of free-living nematodes as a bioassay for estuarine sediments

Tietjen, John H.; Lee, John J.

doi:10.1016/0141-1136(84)90037-0

Cited by 39 publications

(22 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The short generation time of some nematode species means that the composition of the nematode fauna can react rapidly to disturbances (Ferris & Ferris 1979, Platt & Warwick 1980, Platt et al 1984, Tietjen & Lee 1984, Lambshead 1986, Bongers 1988.…”

Section: O Inter-research/printed In Germanymentioning

confidence: 99%

Interpretation of disturbance-induced maturity decrease in marine nematode assemblages by means of the Maturity Index

Bongers¹,

Alkemade²,

Yeates³

1991

Mar. Ecol. Prog. Ser.

314

176

View full text Add to dashboard Cite

Nematode assemblages offer possibilities for the development of a system to assess the 'quality' of aquatic habitats Assessment of disturbances in brackish and marine habitats is currently based on abundance patterns or the ratio between Wieser's feeding types 1 B and 2A. The Maturity Index, based on nematode life strategies, has been shown to offer good potential to detect disturbances in terrestrial and freshwater habitats. In this study marine nematode genera from the Dutch coast are classified from colonisers to persisters and the versatility of this c-p scale is demonstrated using published data (organic pollution, oil spill, heavy metals and recolonisation). These examples suggest that the nematode Maturity Index may also be useful in assessing disturbance of marine and brackish water sediments.

show abstract

Section: O Inter-research/printed In Germanymentioning

confidence: 99%

Interpretation of disturbance-induced maturity decrease in marine nematode assemblages by means of the Maturity Index

Bongers¹,

Alkemade²,

Yeates³

1991

Mar. Ecol. Prog. Ser.

314

176

View full text Add to dashboard Cite

show abstract

“…They are becoming increasingly important as indicators for environmental monitoring, particularly in relation to marine pollution (Tietjen & Lee 1984, Lambshead 1986, Austen & McEvoy 1997, Bongers & Ferris 1999, Boyd et al 2000, Ahnert & Schriever 2001. Despite such attributes, nematode taxonomy is relatively time-consuming, making studies of nematode diversity in marine ecosystems somewhat laborious (Coomans 2002, Floyd et al 2002, Cook et al 2005.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of marine nematodes from environmental samples: overcoming eukaryotic interference

Austen

Bilton²,

Lambshead³

et al. 2006

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

Nematodes form an important and dominant component of many benthic marine ecosystems, but are frequently neglected by marine ecologists because of the time-consuming nature of their identification. Molecular techniques provide powerful tools for the rapid assessment of biodiversity, although few attempts have been made to apply these to marine meiofauna. We evaluated the success of 2 primer sets in amplifying nematode 18S rRNA from DNA templates extracted directly from marine and estuarine sediments. PCR products were separated using denaturing gradient gel electrophoresis (DGGE), and some of the intense DGGE bands were excised, cloned and sequenced to confirm their nematode origin. Initially, other eukaryotic 18S rRNA regions co-amplified with those from nematodes, possibly as a result of the high relative abundance and biomass of other organisms in the studied sediments. These problems were overcome by designing and evaluating consensus primers that selectively amplified nematode ribosomal regions from environmental DNA. Approximately 10 to 12 taxa from each site were detected in the denaturing gel in this study. Tentative affiliations of some the DGGE bands re-amplified using nematode-specific primers were determined by comparing with known marine nematode 18S rRNA sequences in a phylogenetic tree. Our study demonstrates for the first time that PCR combined with DGGE can be used to explore the community composition of many meiofaunal groups, such as nematodes, from DNA extracted directly from environmental samples. KEY WORDS: Marine nematodes · Environmental DNA · Ribosomal primers · Denaturing gradient gel electrophoresis · DGGE · Eukaryotic interference · Diversity Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 44: [97][98][99][100][101][102][103] 2006 niques have been successfully applied to address questions of diversity in marine eukaryotes such as protists and picoplanktons (Díez et al. 2001, Gast et al. 2004, Countway et al. 2005. To date, only Cook et al. (2005) have used electrophoretic techniques to study marine nematode diversity. This study relied on extraction of nematodes from sediment prior to ribosomal RNA amplification using universal primers. Meldal (2004) and Cook et al. (2005) used 18S rRNA for barcoding of marine nematodes and molecular phylogenetics, respectively, but there is no report as yet on the use of nematode-specific 18S rRNA primers for amplifying environmental DNA directly extracted from marine and estuarine sediments.The aim of this study was to establish and extend the potential of molecular ecological approaches for rapid screening of marine nematode diversity from natural environments, and specifically to determine whether nematode diversity could be rapidly assessed from DNA extracted directly from sediments. We employed the 18S rRNA gene as a marker of nematode diversity in this study since its sequences are generally species specific and it contains both conserved (primer design) and variable (taxonomic dis...

show abstract

“…Two marine nematode bioassays have been thus far developed (Table 3) . These can be used to analyse both marine and estuarine sediments (Tietjen & Lee, 1984) .…”

Section: Marine Nematodamentioning

confidence: 99%

Toxicity analysis of freshwater and marine sediments with meio- and macrobenthic organisms: a review

Traunspurger

Drews

1996

Hydrobiologia

View full text Add to dashboard Cite

Benthic metazoans play a key role as test organisms in toxicity analyses of aquatic ecosystems . This report gives an overview of the species of benthic metazoans used for the assessment of toxicity in freshwater and marine sediments, as well as of the criteria relevant to the choice between test species and procedures . The main applications of these organisms are mono-species bioassays, test-batteries, analyses of benthic communities and bioaccumulation studies . Sediment toxicity assays, including acute and chronic exposures, have been developed for nematodes, insects, oligochaetes, polychaetes, crustaceans, molluscs and echinoderms . At least 30 species of freshwater and 71 species of marine and estuarine benthic metazoans have thus far been used in sediment toxicity bioassays . Although aquatic pollution is a world-wide problem, most sediment toxicity bioassays have been developed for organisms native to Europe and North America . The most common bioassay endpoints are mortality, development, growth and behavioural responses . The value of genetic, biochemical, physiological and pathological responses as toxicity endpoints is currently being investigated . The quest for additional test species and protocols is still a worthwhile endeavour in sediment ecotoxicology . Abstract 215Introduction 215

show abstract

The use of free-living nematodes as a bioassay for estuarine sediments

Cited by 39 publications

References 5 publications

Interpretation of disturbance-induced maturity decrease in marine nematode assemblages by means of the Maturity Index

Interpretation of disturbance-induced maturity decrease in marine nematode assemblages by means of the Maturity Index

Molecular detection of marine nematodes from environmental samples: overcoming eukaryotic interference

Toxicity analysis of freshwater and marine sediments with meio- and macrobenthic organisms: a review

Contact Info

Product

Resources

About