The use of formic acid as a common solvent for electrospinning of hybrid PHB/Soy protein fibers

Budurova, Dessislava; Ublekov, Filip; Penchev, Hristo

doi:10.1016/j.matlet.2021.130313

Cited by 16 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To overcome these drawbacks, some strategies have been proposed, such as protein crosslinking and/or blending with synthetic polymers. [12][13][14][15] Most of the SPI-based nanofibers reported in the literature are water-soluble systems and require a crosslinking reaction to improve their water stability. Typically, bifunctional crosslinkers, such as aldehyde compounds, commonly react with reactive groups present in the protein chain (i.e., primary amine, carboxyl, hydroxyl, and sulfhydryl groups) and are often used as crosslinking agents.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of thermal treatments and UV radiation on green soy protein isolated crosslinked electrospun mats

Cunha

Ponce

Abraham

2023

J of Applied Polymer Sci

View full text Add to dashboard Cite

Plant‐derived proteins continue to draw great interest due to their high availability, biodegradability, and biological properties. Among them, soy protein isolate (SPI) has been explored for the development of different sustainable and biodegradable materials in many bionanotechnological fields. In addition, the electrospinning technique has gained interest as a versatile platform for designing synthetic/biopolymer‐based nanofibrous mats for a huge variety of bionanotechnological fields. However, the water stability of SPI mats is low, and the sterilization procedures and conditions are not well established. In this work, oxidized sucrose (OS) was used as a green and inexpensive crosslinker for electrospun SPI mats. The sugar oxidation process was optimized by varying hydrogen peroxide concentration and reaction temperature. SPI‐OS solutions were electrospun using acetic acid as a benign solvent and the resulting mats were thermally treated at mild temperatures and different times. The crosslinked structures were fully characterized in terms of fiber morphology, and physicochemical and thermal properties. Interestingly, uniform bead‐free nanofibrous SPI‐OS membranes that retained their fibrous morphology after 7 days of immersion in phosphate‐buffered saline were successfully obtained. In addition, bilayered SPI‐OS/polycaprolactone mats were also produced by green electrospinning and sterilized by UV radiation for different exposure times. Finally, the effect of sterilization on the relevant properties was presented and discussed. UV radiation demonstrated to be an effective method for sterilizing crosslinked SPI‐based nanofibrous structures.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Electrospun SPI systems nonetheless show low mechanical integrity and low water stability. To overcome these drawbacks, some strategies have been proposed, such as protein crosslinking and/or blending with synthetic polymers 12–15 …”

Section: Introductionmentioning

confidence: 99%

Effect of thermal treatments and UV radiation on green soy protein isolated crosslinked electrospun mats

Cunha

Ponce

Abraham

2023

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…In this article, formic acid (FA) was employed as a benign (green) solvent for the first time because it has good dissolving capacity of both PCL and PVA at different ratios (0%/100%, 25%/75%, 50%/50%, 75%/25%, and 100%/0%). In literatures, green solvents usually have lower toxicity compared with common halogen‐containing solvents 2,18 . Moreover, FA is not only a solvent but also a good modifier.…”

Section: Introductionmentioning

confidence: 99%

Potential of uniaxial electrospun composite nanofibers based on polycaprolactone and polyvinyl alcohol in guided bone regeneration

Jiang

Liu

Cheng

et al. 2023

J of Applied Polymer Sci

View full text Add to dashboard Cite

Polycaprolactone and polyvinyl alcohol are commonly used polymers with biocompatibility and biodegradability. However, the poor mechanical properties of polycaprolactone and the high swelling properties of polyvinyl alcohol hinder their application in guided tissue/bone regeneration. In this study, a new strategy for preparing absorbable guided bone regeneration (GBR) membrane was innovatively put forward, which was to simply blend them in formic acid in different proportions (0%/100%, 25%/75%, 50%/50%, 75%/25%, and 100%/0% wt/wt) and obtain nanofiber membrane by uniaxial electrospinning. The nano‐structure was confirmed by scanning electron microscope, and the change of chemical functional groups was confirmed by Fourier transform infrared spectroscopy. In addition, the proper polymer proportion (75%/25%) was selected through some tests. The results show that the nanofiber membrane has suitable wettability (104.083 ± 2.512°), sufficient Young's modulus (90.895 ± 7.431 MPa), the lowest swelling rate (33.705 ± 1.806%) and shrinkage rate (1.878 ± 3.252%), and no cytotoxicity. Its biodegradability and osteogenic effects in vitro were also investigated to show the potential applied in GBR.

show abstract

“…Among these, poly(3-hydroxybutyrate) [P(3HB)] is the most abundant PHA in nature and is produced by many microbes. Although the brittleness of P(3HB) has limited its practical use, recent studies have developed several applications by blending P(3HB) with other polymers, such as soy protein fibers [ 14 ], chitosans [ 15 ], and PLAs [ 16 ]. Another effective strategy to reduce brittleness is random copolymerization, which induces increased ductility of the polymer.…”

Section: Introductionmentioning

confidence: 99%

Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR

et al. 2022

View full text Add to dashboard Cite

Background Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence. The development of PhaCAR, a unique sequence-regulating PHA synthase, has enabled the spontaneous biosynthesis of PHA block copolymers. PhaCAR synthesizes both a block copolymer poly(2-hydroxybutyrate)-b-poly(3-hydroxybutyrate) [P(2HB)-b-P(3HB)], and a random copolymer, poly(3HB-co-3-hydroxyhexanoate), indicating that the combination of monomers determines the monomer sequence. Therefore, in this study, we explored the substrate scope of PhaCAR and the monomer sequences of the resulting copolymers to identify the determinants of the monomer sequence. PhaCAR is a class I PHA synthase that is thought to incorporate long-main-chain hydroxyalkanoates (LMC HAs, > C3 in the main [backbone] chain). Thus, the LMC monomers, 4-hydroxy-2-methylbutyrate (4H2MB), 5-hydroxyvalerate (5HV), and 6-hydroxyhexanoate (6HHx), as well as 2HB, 3HB, and 3-hydroxypropionate (3HP) were tested. Results Recombinant Escherichia coli harboring PhaCAR, CoA transferase and CoA ligase genes was used for PHA production. The medium contained the monomer precursors, 2HB, 3HB, 3HP, 4H2MB, 5HV, and 6HHx, either individually or in combination. As a result, homopolymers were obtained only for 3HB and 3HP. Moreover, 3HB and 3HP were randomly copolymerized by PhaCAR. 3HB-based binary copolymers P(3HB-co-LMC HA)s containing up to 2.9 mol% 4H2MB, 4.8 mol% 5HV, or 1.8 mol% 6HHx were produced. Differential scanning calorimetry analysis of the copolymers indicated that P(3HB-co-LMC HA)s had a random sequence. In contrast, combining 3HP and 2HB induced the synthesis of P(3HP)-b-P(2HB). Similarly, P(2HB) segment-containing block copolymers P(3HB-co-LMC HA)-b-P(2HB)s were synthesized. Binary copolymers of LMC HAs and 2HB were not obtained, indicating that the 3HB or 3HP unit is essential to the polymer synthesis. Conclusion PhaCAR possesses a wide substrate scope towards 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates. 3HB or 3HP units are essential for polymer synthesis using PhaCAR. The presence of a 2HB monomer is key to synthesizing block copolymers, such as P(3HP)-b-P(2HB) and P(3HB-co-LMC HA)-b-P(2HB)s. The copolymers that did not contain 2HB units had a random sequence. This study’s results provide insights into the mechanism of sequence regulation by PhaCAR and pave the way for designing PHA block copolymers.

show abstract

The use of formic acid as a common solvent for electrospinning of hybrid PHB/Soy protein fibers

Cited by 16 publications

References 13 publications

Effect of thermal treatments and UV radiation on green soy protein isolated crosslinked electrospun mats

Effect of thermal treatments and UV radiation on green soy protein isolated crosslinked electrospun mats

Potential of uniaxial electrospun composite nanofibers based on polycaprolactone and polyvinyl alcohol in guided bone regeneration

Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR

Contact Info

Product

Resources

About