The use of fluorimetry in the automated assay of Ittrich's total oestrogens in the urine of pregnant women

Comparison of an Automated Fluorimetric Method with Two Manual Methods for the Determination of Pregnancy Oestrogens

“…The automated fluorometric assay of urinary oestrogens was carried out at the Luton and Dunstable Hospital using the method of Muir et al (1969). The procedure of Oakey et al, method B (1967) was carried out, as described by the authors, at the Selly Oak Hospital, Birmingham.…”

“…Ua Conaill and Muir (1968) and Muir, ua Conaill and Ryan (1969) described an automated continuous flow system for the determination of oestrogens in pregnancy urine, based on the fluorometric method of Ittrich (1958). In the original publication of ua Conaill and Muir the results obtained by the automated procedure were compared with the manual Ittrich method, as modified by Cartlidge et al (1961).…”

Fluorometric analysis

“…Simplified fluorometric methods were studied and improvements suggested (127, 156). Automated procedures were described for the assay of urinary estrogens in nonpregnancy (646), pregnancy (444), and late pregnancy (709), and for ethinylestradiol in pharmaceutical preparations (336).…”