The use of fluorescent reporter protein tagging to study the interaction between Root-Knot Nematodes and Soft Rot Enterobacteriaceae

Mongae, Aobakwe Oratile; Kubheka, Gugulethu Cresentia; Moleleki, Ntsane; Moleleki, Lucy N.

doi:10.1111/lam.12045

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…Also proteins annotated as chitinase and chitin binding protein were among the identified P. parvum -specific proteins. Soft rot Pectobacteriaceae have been identified in numerous insect species [ 34 ] and in slugs [ 35 ], and they can also be vectored by free living [ 36 ] and root-knot nematodes [ 37 ]. The characteristics of the P. parvum -specific genes may suggest that it forms a more intimate relationship with a vector when compared to P. polaris or other Pectobacteriaceae species.…”

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confidence: 99%

Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence

Pasanen

Waleron

Schott

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Pectobacterium strains isolated from potato stems in Finland, Poland and the Netherlands were subjected to polyphasic analyses to characterize their genomic and phenotypic features. Phylogenetic analysis based on 382 core proteins showed that the isolates clustered closest to Pectobacterium polaris but could be divided into two clades. Average nucleotide identity (ANI) analysis revealed that the isolates in one of the clades included the P. polaris type strain, whereas the second clade was at the border of the species P. polaris with a 96 % ANI value. In silico genome-to-genome comparisons between the isolates revealed values below 70%, patristic distances based on 1294 core proteins were at the level observed between closely related Pectobacterium species, and the two groups of bacteria differed in genome size, G+C content and results of amplified fragment length polymorphism and Biolog analyses. Comparisons between the genomes revealed that the isolates of the atypical group contained SPI-1-type Type III secretion island and genes coding for proteins known for toxic effects on nematodes or insects, and lacked many genes coding for previously characterized virulence determinants affecting rotting of plant tissue by soft rot bacteria. Furthermore, the atypical isolates could be differentiated from P. polaris by their low virulence, production of antibacterial metabolites and a citrate-negative phenotype. Based on the results of a polyphasic approach including genome-to-genome comparisons, biochemical and virulence assays, presented in this report, we propose delineation of the atypical isolates as a novel species Pectobacterium parvum, for which the isolate s0421 T (CFBP 8630 T =LMG 30828 T) is suggested as a type strain.

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confidence: 99%

Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence

Pasanen

Waleron

Schott

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…and pathogens such as Fusarium spp. are well documented, while there may still be many other interactions which are less well studied (Siddiqui et al, 2010;Mongae et al, 2013).…”

Section: Economic Impactmentioning

confidence: 99%

The threat of root‐knot nematodes (Meloidogyne spp.) in Africa: a review

et al. 2014

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Meloidogyne species pose a significant threat to crop production in Africa due to the losses they cause in a wide range of agricultural crops. The direct and indirect damage caused by various Meloidogyne species results in delayed maturity, toppling, reduced yields and quality of crop produce, high costs of production and therefore loss of income. In addition, emergence of resistance-breaking Meloidogyne species has partly rendered various pest management programmes already in place ineffective, therefore putting food security of the continent at risk. It is likely that more losses may be experienced in the future due to the on-going withdrawal of nematicides. To adequately address the threat of Meloidogyne species in Africa, an accurate assessment and understanding of the species present, genetic diversity, population structure, parasitism mechanisms and how each of these factors contribute to the overall threat posed by Meloidogyne species is important. Thus, the ability to accurately characterize and identify Meloidogyne species is crucial if the threat of Meloidogyne species to crop production in Africa is to be effectively tackled. This review discusses the use of traditional versus molecular-based identification methods of Meloidogyne species and how accurate identification using a polyphasic approach can negate the eminent threat of root knot nematodes in crop production. The potential threat to Africa posed by highly damaging and resistance-breaking populations of 'emerging' Meloidogyne species is also examined.

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“…A glycerol stock of Pectobacterium carotovorum subsp brasiliense (Pcb) transformed with a plasmid (pMP7604) harbouring the red fluorescent protein (mCherry) and tetracycline resistance genes was retrieved from the -70 C freezer (Lagendijk et al, 2010;Mongae et al, 2013). Single colonies of Pcb_mCherry were prepared from stocks by streaking on nutrient agar supplemented with 12.5 µg/ml tetracycline then incubated for two days at 37C.…”

Section: Media and Culture Conditions For Pectobacterium Carotovorum mentioning

confidence: 99%

The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

Mongae

Moleleki

2015

Eur J Plant Pathol

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The efficacy of three elicitors, DL-β-aminobutyric acid (BABA), Acibenzolar-S-methyl (Bion) and Messenger (Harpin) on potato plants inoculated with Meloidogyne incognita second stage juveniles (J2s) was investigated in this study. The efficacy of each elicitor was evaluated based on galling index, egg numbers, total root knot nematode (RKN) population numbers, number of juveniles and the percentage of females in respective extracted populations per treatment at 28 and 56 dpi. There was a significant decrease in galling indices of all treated compared to untreated control plants. The highest percentage reduction was observed for 20 mM BABA-treated plants (33%) followed by 5mM BABA (23%), 50mg/L Bion (18%), 100mg/L Bion (15%) and Messenger (8%). The number of eggs extracted from plants treated with 20mM BABA was significantly lower (66%) compared to untreated controls. Furthermore, treating plants with 20mM BABA decreased the total RKN population numbers by 55% whilst the decrease observed for 2 Bion and Messenger was between 24-39%. Of the three tested elicitors, 20mM BABA was found to be the most effective elicitor at both 28 and 56 dpi. Hence, it was selected for further testing against RKN and Pectobacterium carotovorum subsp. brasiliense (Pcb) disease complex. Efficacy was tested by comparing the colony forming units (cfu) of Pcb from treated and untreated control plants inoculated with M. incongita and Pcb simultaneously. The concentration of Pcb from untreated plants inoculated with both pathogens was significantly higher (3.3 × 10 3 cfu/ml) compared to their treated counterparts (8.71 × 10 2 cfu/ml). This suggests that BABA has the potential to decrease soft rot bacterial infections in RKN disease complexes by decreasing the rate of RKN infection. This is the first report where BABA is shown to decrease the infection rate of RKN as a primary causal agent in combination with a secondary pathogen.

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The use of fluorescent reporter protein tagging to study the interaction between Root-Knot Nematodes and Soft Rot Enterobacteriaceae

Cited by 4 publications

References 16 publications

Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence

Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence

The threat of root‐knot nematodes (Meloidogyne spp.) in Africa: a review

The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

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