The Use of Fluorescence Spectra for the Detection of Scab and Rot in Fruit and Vegetable Crops

Abstract

Using Fluorescence Spectrometer Lumina, fluorescence spectra of surface slices of apples and potatoes were measured. Some of the samples were healthy, some were infected: apples had scabs, potatoes had rot and mechanical damage. For apples, two zones were found where the emission spectra of healthy and scab-affected samples differed significantly from each other. This is the region of 400–450 nm with excitation of 300–350 nm, as well as the region of 680–750 nm with excitation of 400–450 nm. For potatoes, the differences between a healthy and rot-affected sample were found only in the region of 400–450 nm with excitation at 300–350 nm. The found differences are clearly manifested in the correlation coefficients between the spectra - the minimum correlation coefficient for healthy apples and scab at 450 nm excitation r = 0.51. Also, healthy and diseased samples are well separated using principal component analysis (PCA). The revealed differences in the fluorescence spectra can be used for the detection and separation of diseased and healthy fruits and vegetables.

Determination of Main Spectral and Luminescent Characteristics of Winter Wheat Seeds Infected with Pathogenic Microflora

“…The occurrence of diseases affects the fluorescence of phenolic compounds and chlorophyll in grapefruit fruit [18] and other citrus fruit [19]. The fluorescence spectra of the surface parts of healthy and infected apples and potatoes were studied [20]. Two zones were found for apples, where the radiation spectra of healthy samples and samples affected by scab differed significantly from each other.…”

Selenium Nanoparticles Can Influence the Immune Response Due to Interactions with Antibodies and Modulation of the Physiological State of Granulocytes

“…Each sample was measured three times. The figures show typical spectra; with repeated measurements, the intensity maxima change by several percent [56]. Refractive index measurements were carried out on a Multiwavelengths Refractometer: Abbemat MW (Anton Paar, Graz, Austria).…”

Intrinsic Fluorescence Markers for Food Characteristics, Shelf Life, and Safety Estimation: Advanced Analytical Approach

“…The healthy and diseased samples could be successfully separated by PCA applied on the emission spectra. The results indicate that fluorescence spectroscopy can be used to detect and separate diseased and healthy fruits and vegetables [ 71 ].…”