Abstract:Flow cell cytometry is reported here as a rapid fluorescent immunoassay method to characterize monoclonal antibody production in hybridoma cultures. Actinomyces viscosus was the bacterium chosen as a model to illustrate this procedure. Fluorescein isothiocyanate (FITC), coupled to staphylococcal Protein A (PA), was used as the fluorescent marker to detect and quantitate antigen‐antibody reactions. Flow cell cytometry was also used with rabbit polyclonal antibodies to A. viscosus coupled with PAFITC for compari… Show more
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