The use of field enclosure experiments to study the effect of pesticides on lake phytoplankton

Abdelhamid, Mohamed I.; Källqvist, Torsten; Hessen, Dag O.; Berge, D.

doi:10.1111/j.1440-1770.1996.tb00065.x

Cited by 6 publications

(1 citation statement)

References 14 publications

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“…However, some species can be highly sensitive to fungicides (Abdel-Hamid et al 1996). As far as we know, only one study reported adverse effects of epoxiconazole on a phytoplankton species: after a 24 h exposure to 100 lg l -1 of its commercial formulation Opus, the dinoflagellate Karenia mikimotoı¨had significantly increased extent of DNA strand breaks (Akcha et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Stachowski-Haberkorn

Quiniou²,

Beker³

et al. 2008

Ecotoxicology

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The impacts of the fungicide Opus (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 microg l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 microg l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant differences in microcosms exposed to 10 microg l(-1) of epoxiconazole. When exposed to 100 microg l(-1), all three methods allowed to detect significant differences in treated microcosms, as compared to controls. The TTGE analysis appears in this study as the most sensitive method for fungicide impact assessment on eukaryote microbial communities.

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Section: Introductionmentioning

confidence: 98%

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Stachowski-Haberkorn

Quiniou²,

Beker³

et al. 2008

Ecotoxicology

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Effects of Organic Herbicides on Phototrophic Microbial Communities in Freshwater Ecosystems

Pesce¹,

Bouchez²,

Montuelle³

2011

Reviews of Environmental Contamination and Toxicology

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Over the past 15 years, significant research efforts have been channeled into assessing the effects of organic herbicides on freshwater phototrophic microbial communities. The results of this research are reviewed herein. The main conclusions we have reached after performing this review can be summarized into five points: · Most relevant assessments have dealt with the effects of triazine and phenylurea herbicides. Herbicides from these chemical classes are often considered to be model compounds when photosystem-II inhibitors are studied. · Until the early 2000s, the vast majority of investigations conducted to evaluate herbicide effects on phototropic microbes were performed in microcosms or mesocosms. In such studies, herbicides were usually applied alone, and often at concentrations much higher than those detected in the environment. More recently, the trend has been toward more realistic and relevant studies, in which lower herbicide concentrations were considered, and compound mixtures or successive treatments were tested. Increasingly, in situ studies are being designed to directly evaluate microbial community responses, following chemical exposures in contaminated aquatic environments. · Several biological end points are used to evaluate how organisms in the phototrophic microbial community respond to herbicide exposure. These end points allow the detection of quantitative changes, such as chl a concentrations, total cell counts or periphytic biomass, qualitative changes such as community structure to algal diversity, or functional changes such as photosynthesis and respiration, among others. They may give different and complementary information concerning the responses of microbial communities. · PICT approaches, which have generally combined functional and structural measurements, may prove to be valuable for assessing both an immediate impact, and for factoring in the contamination history of an ecosystem at the community level. · Finally, any relevant assessment of pesticide effects should incorporate a detailed environmental characterization that would include abiotic parameters (light, flow speed, nutrient content), or biotic parameters (diversity and structure of biofilms), because these control the bioavailability of pesticides, and thereby the exposure of microbial communities. To improve the value of ecotoxicological risk assessments, future research is needed in two key areas: first, more information on the effects of pollutants at the community level must be obtained (new tools and new end points), and second, more effort must be directed to reinforce the ecological relevance of toxicological investigations.

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Indicators for Monitoring Aquatic Ecosystem

2017

Periphyton

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The use of field enclosure experiments to study the effect of pesticides on lake phytoplankton

Cited by 6 publications

References 14 publications

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

Effects of Organic Herbicides on Phototrophic Microbial Communities in Freshwater Ecosystems

Indicators for Monitoring Aquatic Ecosystem

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