The Use of Fetal Bovine Serum: Ethical or Scientific Problem?

Jochems, Carlo E A; Valk, J.B.F. van der; Stafleu, Frans; Baumans, V.

doi:10.1177/026119290203000208

Cited by 214 publications

(161 citation statements)

References 54 publications

(47 reference statements)

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“…For instance, media composition and seeding density can have a significant influence on the permeability and differentiation of human Caco-2 cells [20]. Batch to batch fluctuation in fetal bovine serum composition is a well documented phenomenon which can interfere with key genotypic and phenotypic cell stability and other key functions such as cell spreading, attachment and differentiation [4,21]. Bulk passage techniques, involving enzymatic disaggregation (trypsin/collagenase), had a significant effect on the genetic stability of human stem cells [5].…”

Section: Resultsmentioning

confidence: 99%

The effect of sub-culturing on the basal level of type I interferon (IFN) gene expression in the Salmon Head Kidney (SHK-1) cell line

Fourrier¹,

Arnold²,

Collet³

et al. 2009

Fish & Shellfish Immunology

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Section: Resultsmentioning

confidence: 99%

The effect of sub-culturing on the basal level of type I interferon (IFN) gene expression in the Salmon Head Kidney (SHK-1) cell line

Fourrier¹,

Arnold²,

Collet³

et al. 2009

Fish & Shellfish Immunology

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“…The medium used for the MSCs culture and propagation is crucial for the safety and efficacy of MSCs and ideally should maintain their phenotype, differentiation potential and functionality during multiple passages. In the majority of studies involving MSCs, comparative analyses between different MSCs types have been done in medium supplemented with fetal bovine serum (FBS), which presents several disadvantages both for the high batch-to-batch variability, that can lead to low quality and scarce reproducibility of culture performance, and for the low safety for cell therapy use, due to the risks of xenogeneic immune reactions against bovine antigens and animal pathogen transmissions [47, 48]. To date, several human supplements have been tested as alternatives to FBS for MSC culture.…”

Section: Introductionmentioning

confidence: 99%

Potency testing of mesenchymal stromal cell growth expanded in human platelet lysate from different human tissues

et al. 2016

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BackgroundMesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different sources, such as bone marrow (BM), umbilical cord tissue (UCT) and adipose tissue (AT), share many biological features, although they may show some differences on cumulative yield, proliferative ability and differentiation potential. The standardization of MSCs growth and their functional amplification is a mandatory objective of cell therapies. The aim of this study was to evaluate the cumulative yield and the ex vivo amplification potential of MSCs obtained from various sources and different subjects, using defined culture conditions with a standardized platelet lysate (PL) as growth stimulus.MethodsMSCs isolated from BM, UCT and AT and expanded in human PL were compared in terms of cumulative yield and growth potential per gram of starting tissue. MSCs morphology, phenotype, differentiation potential, and immunomodulatory properties were also investigated to evaluate their biological characteristics.ResultsThe use of standardized PL-based culture conditions resulted in a very low variability of MSC growth. Our data showed that AT has the greater capacity to generate MSC per gram of initial tissue, compared to BM and UCT. However, UCT-MSCs replicated faster than AT-MSCs and BM-MSCs, revealing a greater proliferation capacity of this source irrespective of its lower MSC yield. All MSCs exhibited the typical MSC phenotype and the ability to differentiate into all mesodermal lineages, while BM-MSCs showed the most prominent immunosuppressive effect in vitro.ConclusionsThe adoption of standardized culture conditions may help researchers and clinicians to reveal particular characteristics and inter-individual variability of MSCs sourced from different tissues. These data will be beneficial to set the standards for tissue collection and MSCs clinical-scale expansion both for cell banking and for cell-based therapy settings.

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“…Little is known about the effect of FCS on either the sandwich model or the multicellular spheroid model of hepatocytes. Although fetal calf serum (FCS) is a supplement of many cell culture media, providing many necessary growth factors and cytokines for successful culture, many substances present in FCS have not yet been clearly defined, nor are the functions of the cultured cells [40] and the exact effect of serum on the liver cell culture always understood [41]. Wessman and Levings, [42] reported that as much as 20–50% of commercial fetal bovine serum was virus-positive.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Functional Analysis of Hepatocyte Spheroids Generated on Poly-HEMA-Treated Surfaces under the Influence of Fetal Calf Serum and Nonparenchymal Cells

Açıkgöz

Giri²,

Cho

et al. 2013

Biomolecules

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Poly (2-hydroxyethyl methacrylate) (HEMA) has been used as a clinical material, in the form of a soft hydrogel, for various surgical procedures, including endovascular surgery of liver. It is a clear liquid compound and, as a soft, flexible, water-absorbing material, has been used to make soft contact lenses from small, concave, spinning molds. Primary rat hepatocyte spheroids were created on a poly-HEMA-coated surface with the intention of inducing hepatic tissue formation and improving liver functions. We investigated spheroid formation of primary adult rat hepatocyte cells and characterized hepatic-specific functions under the special influence of fetal calf serum (FCS) and nonparencymal cells (NPC) up to six days in different culture systems (e.g., hepatocytes + FCS, hepatocytes – FCS, NPC + FCS, NPC – FCS, co-culture + FCS, co-culture – FCS) in both the spheroid model and sandwich model. Immunohistologically, we detected gap junctions, Ito cell/Kupffer cells, sinusoidal endothelial cells and an extracellular matrix in the spheroid model. FCS has no positive effect in the sandwich model, but has a negative effect in the spheroid model on albumin production, and no influence in urea production in either model. We found more cell viability in smaller diameter spheroids than larger ones by using the apoptosis test. Furthermore, there is no positive influence of the serum or NPC on spheroid formation, suggesting that it may only depend on the physical condition of the culture system. Since the sandwich culture has been considered a “gold standard” in vitro culture model, the hepatocyte spheroids generated on the poly-HEMA-coated surface were compared with those in the sandwich model. Major liver-specific functions, such as albumin secretion and urea synthesis, were evaluated in both the spheroid and sandwich model. The synthesis performance in the spheroid compared to the sandwich culture increases approximately by a factor of 1.5. Disintegration of plasma membranes in both models was measured by lactate dehydrogenase (LDH) release in both models. Additionally, diazepam was used as a substrate in drug metabolism studies to characterize the differences in the biotransformation potential with metabolite profiles in both models. It showed that the diazepam metabolism activities in the spheroid model is about 10-fold lower than the sandwich model. The poly-HEMA-based hepatocyte spheroid is a promising new platform towards hepatic tissue engineering leading to in vitro hepatic tissue formation.

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The Use of Fetal Bovine Serum: Ethical or Scientific Problem?

Cited by 214 publications

References 54 publications

The effect of sub-culturing on the basal level of type I interferon (IFN) gene expression in the Salmon Head Kidney (SHK-1) cell line

The effect of sub-culturing on the basal level of type I interferon (IFN) gene expression in the Salmon Head Kidney (SHK-1) cell line

Potency testing of mesenchymal stromal cell growth expanded in human platelet lysate from different human tissues

Morphological and Functional Analysis of Hepatocyte Spheroids Generated on Poly-HEMA-Treated Surfaces under the Influence of Fetal Calf Serum and Nonparenchymal Cells

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