2019
DOI: 10.1016/j.actatropica.2019.01.027
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The use of ErpY-like recombinant protein from Leptospira interrogans in the development of an immunodiagnostic test for swine leptospirosis

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Cited by 8 publications
(13 citation statements)
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“…To date, the role of ErpY-like protein in Leptospira pathogenesis has not been known; however, it is a potential vaccine and diagnostic candidate for leptospirosis in the specific host (18,19). We demonstrate that ErpY-like protein of Leptospira is a surface-exposed outer membrane lipoprotein, and its recombinant form of antigen can be implemented in serological diagnosis of leptospirosis in humans, bovines, and canines.…”
Section: Discussionmentioning
confidence: 92%
See 1 more Smart Citation
“…To date, the role of ErpY-like protein in Leptospira pathogenesis has not been known; however, it is a potential vaccine and diagnostic candidate for leptospirosis in the specific host (18,19). We demonstrate that ErpY-like protein of Leptospira is a surface-exposed outer membrane lipoprotein, and its recombinant form of antigen can be implemented in serological diagnosis of leptospirosis in humans, bovines, and canines.…”
Section: Discussionmentioning
confidence: 92%
“…Such binding proteins benefit the bacteria in intervening thrombin-catalyzed clot formation or inhibiting complement activation, essential for successful establishment in the host and impeding the innate defense system. In a recent study, a protein annotated ErpY-like (LIC11966) in Leptospira was demonstrated to be an Fg-binding protein with diagnostic and subunit vaccine potential (17)(18)(19). The ErpY protein annotation originated from outer surface protein E/F-related proteins of another pathogenic spirochete, Borrelia burgdorferi (20).…”
mentioning
confidence: 99%
“…The widely accepted minimum final dilution microscopic agglutination test (MAT) titre considered to be significant is 1:100, with titres ≥1:100 considered positive [ 11 , [20] , [21] , [22] ]. Using this titre as the cut-off, there was no serological evidence of active Leptospira infection or previous seroconversion in any of the 105 swine included in the present serological survey.…”
Section: Discussionmentioning
confidence: 99%
“…Swine are maintenance hosts for a range of Leptospira serovars including Canicola, Grippotyphosa, Icterohaemorrhagiae, and Pomona [ 11 ] while swine disease is associated with serovars Australis, Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, Pomona and Tarassovi [ 12 ]. Swine leptospirosis can be an endemic infection with little evidence of disease, but when first introduced to a naïve herd, or a herd with waning immunity, infertility, abortion, neonatal death and weakness can ensue, while leptospires can persist in the kidney and genital tract of carrier swine [ 12 ].…”
Section: Introductionmentioning
confidence: 99%
“…The development of diagnostic tests using recombinant proteins as ELISA antigens for leptospirosis in humans and other mammals was reported previously 24,25,36,37,54 . Most of the reported serological tests used proteins that are commonly found in pathogenic strains of Leptospira , with sensitivities ranging from 40% to 90% and specificity ranging from 75% to 100%.…”
Section: Discussionmentioning
confidence: 99%