The use of electric fields in tissue engineering

Markx, Gerard H.

doi:10.4161/org.5799

Cited by 73 publications

(63 citation statements)

References 75 publications

(85 reference statements)

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“…The magnitude of the signal is 20 V pp in both cases. Applications of cell positioning include analysis of cell networks [29], drug screening [30] and tissue engineering [31]. The addition of a flow in the channel introduces a force (drag) that competes with the DEP force and expands the applications of cell positioning since more complex functions such as filtering and focusing can be implemented.…”

Section: Particle Positioningmentioning

confidence: 99%

A novel approach to dielectrophoresis using carbon electrodes

2011

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Carbon-electrode dielectrophoresis (carbon-DEP) is demonstrated here as an alternative to more traditional DEP techniques. Carbon-DEP combines advantages of metal-electrode and insulator-based DEP by using low-cost fabrication techniques and low voltages for particle manipulation. The use of 3-D electrodes is proved to yield significant advantages over the use of traditional planar electrodes. This paper details the fabrication of dense arrays of tall high aspect ratio carbon electrodes on a transparent fused-silica substrate. The shrinkage of the SU-8 structures during carbonization is characterized and a design tool for future devices is provided. Applications of carbon electrodes in DEP are then detailed and include particle positioning, high-throughput filtering and cell focusing using positive-DEP. Manipulated cells include Saccharomyces cerevisiae and Drosophila melanogaster. The advantages and disadvantages of carbon-DEP are discussed at the end of this work.

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Section: Particle Positioningmentioning

confidence: 99%

A novel approach to dielectrophoresis using carbon electrodes

2011

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“…Recently, many reports of cell positioning and patterning using electrical fields have been published [20,21]. The dielectrophoretic (DEP) approach is one of the most useful methods because of its ability to move cells in a non-uniform electric field [2224].…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-coated silicone as a flexible and electrically conductive biomedical material

Matsuoka¹,

Akasaka²,

Totsuka³

et al. 2012

Materials Science and Engineering: C

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Artificial cell scaffolds that support cell adhesion, growth, and organization need to be fabricated for various purposes. Recently, there have been increasing reports of cell patterning using electrical fields. We fabricated scaffolds consisting of silicone sheets coated with single-walled (SW) or multi-walled (MW) carbon nanotubes (CNTs) and evaluated their electrical properties and biocompatibility. We also performed cell alignment with dielectrophoresis using CNT-coated sheets as electrodes. Silicone coated with 10 μg/cm 2 SWCNTs exhibited the least sheet resistance (0.8 kΩ/sq); its conductivity was maintained even after 100 stretching cycles. CNT coating also improved cell adhesion and proliferation. When an electric field was applied to the cell suspension introduced on the CNT-coated scaffold, the cells became aligned in a pearl-chain pattern. These results indicate that CNT coating not only provides electro-conductivity but also promotes cell adhesion to the silicone scaffold; cells seeded on the scaffold can be organized using electricity. These findings demonstrate that CNT-coated silicone can be useful as a biocompatible scaffold.

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“…Various other approaches are available which can create 3D distributions in the cell density. These include fluidic, ultrasound, optical, and electrical approaches (Abidin et al, 2007;Albrecht et al, 2005;Birkbeck et al, 2003;Gherardini et al, 2005;Markx, 2008;Mironov et al, 2003;Tan and Desai, 2004;Tsang and Bhatia, 2004). Of those, electrical, and in particular dielectrophoretic approaches, are some of the most useful (Markx, 2008;Markx and Buckle, 2005) because of the relative simplicity of the experiments, the ease of control, and the relatively high spatial resolution that can be achieved.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years the method has attracted increased attention because of its ability to controllably pattern different cell types in a 2D and 3D (micro)environment (Albrecht et al, 2005(Albrecht et al, , 2006(Albrecht et al, , 2007Markx et al, 2009;Sebastian et al, 2007a&b). Reviews of the use of dielectrophoresis-and more generally electrical fields-in tissue engineering have been given previously (Markx, 2008;Markx and Buckle, 2005).…”

Section: Introductionmentioning

confidence: 99%

Creation of arrays of cell aggregates in defined patterns for developmental biology studies using dielectrophoresis

Yusvana

Headon

Markx

2010

Biotech & Bioengineering

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It is shown that dielectrophoresis-the movement of particles in non-uniform electric fields-can be used to create engineered skin with artificial placodes of different sizes and shapes, in different spatial patterns. Modeling of the electric field distribution and image analysis of the cell aggregates produced showed that the aggregation is highly predictable. The cells in the aggregates remain viable, and reorganization and compaction of the cells in the aggregates occurs when the artificial skin is subsequently cultured. The system developed could be of considerable use for the in vitro study of developmental processes where local variations in cell density and direct cell-cell contacts are important. Biotechnol. Bioeng. 2010;105: 945-954.

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The use of electric fields in tissue engineering

Cited by 73 publications

References 75 publications

A novel approach to dielectrophoresis using carbon electrodes

A novel approach to dielectrophoresis using carbon electrodes

Carbon nanotube-coated silicone as a flexible and electrically conductive biomedical material

Creation of arrays of cell aggregates in defined patterns for developmental biology studies using dielectrophoresis

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