The Use of EcoTILLING for the Genetic Improvement of Jatropha curcas L.

Maghuly, Fatemeh; Jankowicz-Cieślak, Joanna; Till, Bradley J.; Laimer, Margit

doi:10.1007/978-1-4614-4915-7_17

Cited by 11 publications

(4 citation statements)

References 27 publications

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“…Three essential steps are necessary for optimal results in TILLING approach; first, the right pooling strategies, second, a good gene model and protein conservation model, which could help to select the gene region with the highest number of possible variations, and third, a suitable PCR primer pairs for a product of approximately 1000–2000 bp. Finally, identified mutations will be confirmed and evaluated by sequencing [ 92 , 93 ]. Ultimately, it can provide an allelic series of silent, mis-sense, non-sense, and splice site mutations to examine the effect of various mutations in a gene.…”

Section: Structure and Mechanism Of Genome Editing Toolsmentioning

confidence: 99%

Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects

Salava

Thula

Mohan

et al. 2021

IJMS

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Plants regularly face the changing climatic conditions that cause biotic and abiotic stress responses. The abiotic stresses are the primary constraints affecting crop yield and nutritional quality in many crop plants. The advances in genome sequencing and high-throughput approaches have enabled the researchers to use genome editing tools for the functional characterization of many genes useful for crop improvement. The present review focuses on the genome editing tools for improving many traits such as disease resistance, abiotic stress tolerance, yield, quality, and nutritional aspects of tomato. Many candidate genes conferring tolerance to abiotic stresses such as heat, cold, drought, and salinity stress have been successfully manipulated by gene modification and editing techniques such as RNA interference, insertional mutagenesis, and clustered regularly interspaced short palindromic repeat (CRISPR/Cas9). In this regard, the genome editing tools such as CRISPR/Cas9, which is a fast and efficient technology that can be exploited to explore the genetic resources for the improvement of tomato and other crop plants in terms of stress tolerance and nutritional quality. The review presents examples of gene editing responsible for conferring both biotic and abiotic stresses in tomato simultaneously. The literature on using this powerful technology to improve fruit quality, yield, and nutritional aspects in tomato is highlighted. Finally, the prospects and challenges of genome editing, public and political acceptance in tomato are discussed.

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Section: Structure and Mechanism Of Genome Editing Toolsmentioning

confidence: 99%

Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects

Salava

Thula

Mohan

et al. 2021

IJMS

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“…Nevertheless, the lack of high genetic variability in J. curcas hampers selective breeding [ 70 ], which calls for other strategies to increase the genetic diversity through chemical/physical mutations or intra/interspecific crossing programs [ 13 ].…”

Section: Breeding Technologies For Jatrophamentioning

confidence: 99%

“…This will allow genome-wide association studies to be conducted and provide a better understanding of the causative mutations of phenotypic variations [ 71 ]. Reverse genetics for haplotyping and SNP discovery like EcoTILLING [ 70 ] and RAD sequencing can make significant contributions to trait correlation, especially for complex trait controlled by multiple genes or QTLs.…”

Section: Breeding Technologies For Jatrophamentioning

confidence: 99%

“…This was recently achieved with TILLING (targeting induced local lesions in genomes), which is a reverse genetics method that can be used for the characterization of in vivo functions of genes. It combines random mutagenesis with high-throughput mutation discovery, provides a spectrum of stable point-alleles and is broadly applicable across most species [ 70 , 87 ].…”

Section: Breeding Technologies For Jatrophamentioning

confidence: 99%

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Jatropha curcas, a biofuel crop: Functional genomics for understanding metabolic pathways and genetic improvement

Maghuly

Laimer

2013

Biotechnology Journal

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Jatropha curcas is currently attracting much attention as an oilseed crop for biofuel, as Jatropha can grow under climate and soil conditions that are unsuitable for food production. However, little is known about Jatropha, and there are a number of challenges to be overcome. In fact, Jatropha has not really been domesticated; most of the Jatropha accessions are toxic, which renders the seedcake unsuitable for use as animal feed. The seeds of Jatropha contain high levels of polyunsaturated fatty acids, which negatively impact the biofuel quality. Fruiting of Jatropha is fairly continuous, thus increasing costs of harvesting. Therefore, before starting any improvement program using conventional or molecular breeding techniques, understanding gene function and the genome scale of Jatropha are prerequisites. This review presents currently available and relevant information on the latest technologies (genomics, transcriptomics, proteomics and metabolomics) to decipher important metabolic pathways within Jatropha, such as oil and toxin synthesis. Further, it discusses future directions for biotechnological approaches in Jatropha breeding and improvement.

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Geographic origin is not supported by the genetic variability found in a large living collection of Jatropha curcas with accessions from three continents

Maghuly

Jankowicz-Cieślak

Pabinger

et al. 2015

Biotechnology Journal

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Increasing economic interest in Jatropha curcas requires a major research focus on the genetic background and geographic origin of this non-edible biofuel crop. To determine the worldwide genetic structure of this species, amplified fragment length polymorphisms, inter simple sequence repeats, and novel single nucleotide polymorphisms (SNPs) were employed for a large collection of 907 J. curcas accessions and related species (RS) from three continents, 15 countries and 53 regions. PCoA, phenogram, and cophenetic analyses separated RS from two J. curcas groups. Accessions from Mexico, Bolivia, Paraguay, Kenya, and Ethiopia with unknown origins were found in both groups. In general, there was a considerable overlap between individuals from different regions and countries. The Bayesian approach using structure demonstrated two groups with a low genetic variation. Analysis of molecular varience revealed significant variation among individuals within populations. SNPs found by in silico analyses of Δ12 fatty acid desaturase indicated possible changes in gene expression and thus in fatty acid profiles. SNP variation was higher in the curcin gene compared to genes involved in oil production. Novel SNPs allowed separating toxic, non-toxic, and Mexican accessions. The present study confirms that human activities had a major influence on the genetic diversity of J. curcas, not only because of domestication, but also because of biased selection.

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The Use of EcoTILLING for the Genetic Improvement of Jatropha curcas L.

Cited by 11 publications

References 27 publications

Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects

Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects

Jatropha curcas, a biofuel crop: Functional genomics for understanding metabolic pathways and genetic improvement

Geographic origin is not supported by the genetic variability found in a large living collection of Jatropha curcas with accessions from three continents

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