“…Specific design for Experiment 5efficacy of pretreatment with acetamiprid or chlorantraniliprole This experiment was designed to investigate the effect of dose rate on the efficacy of chlorantraniliprole, applied as a pre-treatment in the nursery, in protecting young Sitka spruce trees that were subsequently planted out in the forest. The impact on efficacy of adding a dye marker, which is often mixed with diluent when top-up applications are made in forests to make the resulting spray mist more visible to operators, and hence help them to minimise drift, contamination, and non-target runoff (Brown et al 2003), was also tested. In addition, the effectiveness of acetamiprid applied via a new pre-treatment machine developed by the Forestry Commission was examined, to see if the resulting efficacy in protecting planting stock from Hylobius browsing damage in the forest matched that expected from standard research treatments applied by knapsack sprayers.…”