The Use of Dried Blood Spot Sampling in the National Social Life, Health, and Aging Project

Williams, Sharon; McDade, Thomas W.

doi:10.1093/geronb/gbn022

Cited by 92 publications

(81 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All analyses used robust SEs to account for repeated observations. developed for newborn screening programs (44) and has been used in several epidemiologic studies involving blood spot CRP measures (45,46). Assessment.…”

Section: Methodsmentioning

confidence: 99%

“…Serum equivalents therefore were calculated by using the following algorithm based on the serum-blood spot regression: serum [high sensitivity C-reactive protein (hsCRP)] = 1.38*(blood spot CRP value) -0.97. Blood spot CRP measures have been used in several epidemiologic studies (45,46,53). Observations with values above 10 mg/L indicate frank infection and were removed from statistical analysis (n = 109 from a total of 6,000 observations), whereas values below that index the extent of chronic lowgrade systemic inflammation associated with cardiovascular and metabolic risk (54).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Childhood bullying involvement predicts low-grade systemic inflammation into adulthood

Copeland

Wolke

Lereya

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

238

191

View full text Add to dashboard Cite

Significance Bullying is a common childhood experience that affects children at all income levels and racial/ethnic groups. Being a bully victim has long-term adverse consequences on physical and mental health and financial functioning, but bullies themselves display few ill effects. Here, we show that victims suffer from greater increases in low-grade systemic inflammation from childhood to young adulthood than are seen in others. In contrast, bullies showed lower increases in inflammation into adulthood compared with those uninvolved in bullying. Elevated systemic low-grade inflammation is a mechanism by which this common childhood social adversity may get under the skin to affect adult health functioning, even many years later.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Childhood bullying involvement predicts low-grade systemic inflammation into adulthood

Copeland

Wolke

Lereya

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

238

191

View full text Add to dashboard Cite

show abstract

“…This improvement supports the idea that if in future studies adequate training is provided, the DBS collection technique may be used as an alternative to venipuncture in an ambulant setting. [12][13][14] No guidelines are available on the percentage of spots that should fulfill a certain quality standard. As we asked the patients in this study to generate four DBS and as we wished to perform duplicate analysis (i.e., analysis on two different spots on every time point), we put forward an acceptance criterion of 50 % (i.e., there should be at least two DBS with acceptable quality:…”

Section: Discussionmentioning

confidence: 99%

“…Since a maximum of four DBS was requested, DBS cards with at least two suitable DBS were considered to have acceptable quality. [14] For the first collection period, this criterion was fulfilled by 100 % of total cards collected by patient 1, none of the cards by patient 2 (analysis of single spots was however possible on 6 out of 7 days) and 83 % of the cards by patient 3. When repeating the study (series 2), the number of well collected DBS increased, to 71 % by patient 2 and to 100 % by patient 3 (Fig.…”

Section: Dbs Analysismentioning

confidence: 99%

Feasibility of Following up Gamma-Hydroxybutyric Acid Concentrations in Sodium Oxybate (Xyrem®)-Treated Narcoleptic Patients Using Dried Blood Spot Sampling at Home

et al. 2013

View full text Add to dashboard Cite

Background: Gamma-hydroxybutyric acid (GHB), well known as a party drug, especially in Europe, is also legally used (sodium oxybate, Xyrem ® ) to treat a rare sleep disorder, narcolepsy with cataplexy. This exploratory study was set up to measure GHB concentrations in dried blood spots (DBS) collected by narcoleptic patients treated with sodium oxybate. Intra-and inter-individual variation in clinical effects following sodium oxybate administration has been reported. The use of DBS as a sampling technique, which is stated to be easy and convenient, may provide a better insight into GHB concentrations following sodium oxybate intake in a real-life setting. Objective:The aim was two-fold: evaluation of the applicability of a recently developed DBS-based gas chromatography-mass spectrometry (GC-MS) method, and of the feasibility of the sampling technique in an ambulant setting. Methods: Seven narcoleptic patients being treated with sodium oxybate at the Department for RespiratoryDiseases of Ghent University Hospital were asked to collect DBS approximately 20 min after the first sodium oxybate (Xyrem ® ; UCB Pharma Ltd, Brussels, Belgium) intake on a maximum of 7 consecutive days. Using an automatic lancet, patients pricked their fingertip and, after wiping off the first drop of blood, subsequent drops were collected on a DBS card. The DBS cards were sent to the laboratory by regular mail and, before analysis, were visually inspected to record DBS quality (large enough, symmetrically spread on the filter paper with even colouration on both sides of the filter paper). Results:Of the seven patients, three patients succeeded to collect five series of DBS, one patient decided to cease participation because of nausea, one was lost during follow-up and two patients stated falling asleep almost immediately after the intake of sodium oxybate. Analysing the DBS in duplicate resulted in acceptable within-DBS card precision. DBS with acceptable quality were obtained by patients without supervision. Conclusion:Our results demonstrate the acceptable precision of the complete procedure, from sampling at home to quantitative analysis in the laboratory. Given the intra-and inter-individual variability in clinical effects seen with sodium oxybate, the easy adaptation of DBS sampling opens the possibility of following up GHB concentrations in patients in real-life settings in future studies.3

show abstract

“…Nevertheless, the significant linear correlation between matched serum and DBS samples for many analytes (3,20,(27)(28)(29)(30) implies that serum or plasma equivalents can be derived from DBS values (3,28,(30)(31)(32)(33) . It is on this basis that we derived a value of 4?24 to correct the DBS CRP values to serum CRP values.…”

Section: Discussionmentioning

confidence: 99%

The importance of controlling for the acute-phase response in the population-based assessment of vitamin A status: a study in children of pre-school age in Uganda

Baingana

Matovu-Kasozi

Garrett

2012

Public Health Nutr.

View full text Add to dashboard Cite

Objective: To improve estimates of vitamin A deficiency in children of pre-school age in the 2006 Uganda Demographic and Health Survey (UDHS 2006). Design: A cross-sectional study in which dried blood spot samples were analysed for C-reactive protein (CRP). Retinol-binding protein (RBP) had previously been analysed using a commercial enzyme immunoassay. Setting: A population-based study in Uganda. Subjects: A systematically selected subset of the dried blood spot samples collected from children aged 6-59 months for UDHS 2006. Children were categorized into 'normal CRP' (Group A) and 'raised CRP' (Group B) using a CRP cut-off of 5 mg/l. A correction factor was calculated to adjust the Group B RBP values for the influence of the acute-phase response. Results: Geometric mean CRP was 6?2 (95 % CI 5?5, 7?0) mg/l, 1?6 (95 % CI 1?5, 1?8) mg/l and 17?9 (95 % CI 16?4, 19?6) mg/l in all children, in Group A and in Group B, respectively. Geometric mean RBP in all children, in Group A and in Group B was 1?18 (95 % CI 1?14, 1?22) mmol/l, 1?26 (95 % CI 1?20, 1?33) mmol/l and 1?12 (95 % CI 1?07, 1?17) mmol/l, respectively, before correction. Correction increased mean RBP in Group B to 1?26 (95 % CI 1?21, 1?31) mmol/l. The prevalence of vitamin A deficiency (RBP , 0?825 mmol/l) reduced from 18?4 % (95 % CI 17?2, 23?0 %) to 13?9 % (95 % CI 11?3, 16?5 %). Conclusions: Correcting for the acute-phase response significantly reduced the prevalence of vitamin A deficiency; thus, the acute-phase response should be considered when vitamin A status is assessed using RBP in order to improve population-level estimates of vitamin A deficiency. (3) . The VAD prevalence estimate in children was 20?4 % (4) . Circulating concentrations of serum retinol (5)(6)(7) and RBP (8) decline in response to the acute-phase response and may not adequately reflect vitamin A status. These changes, which are an integral part of the acute-phase response, reflect decreased synthesis of RBP and increased excretion of retinol in urine (9,10) . Because levels of circulating acute-phase proteins (APP) can aid in the interpretation of low serum retinol levels, the simultaneous measurement of retinol and one or more APP is recommended (5)(6)(7)(11)(12)(13) . There is currently no consensus on how to correct retinol concentrations for the influence of the acute-phase response. Paracha et al. (11) proposed the application of new cut-offs for low and deficient retinol concentrations for children in whom a 1 -antichymotrypsin and a 1 -acid glycoprotein concentrations were elevated. In the approach proposed by Thurnham et al., individuals with normal APP levels provide the reference group for whom it is assumed that diet is the main factor responsible for the concentration of retinol. The median retinol value for the reference group is divided by the respective median values for groups at different stages of the acutephase response. Correction factors thus obtained were applied to apparently healthy HIV-1-positive adults with raised APP; the proportion with low...

show abstract

The Use of Dried Blood Spot Sampling in the National Social Life, Health, and Aging Project

Cited by 92 publications

References 27 publications

Childhood bullying involvement predicts low-grade systemic inflammation into adulthood

Childhood bullying involvement predicts low-grade systemic inflammation into adulthood

Feasibility of Following up Gamma-Hydroxybutyric Acid Concentrations in Sodium Oxybate (Xyrem®)-Treated Narcoleptic Patients Using Dried Blood Spot Sampling at Home

The importance of controlling for the acute-phase response in the population-based assessment of vitamin A status: a study in children of pre-school age in Uganda

Contact Info

Product

Resources

About