The use of DNA methylation clock in aging research

Liu, Jiaojiao; Liu, Bo; Shi, Jingshan

doi:10.1177/1535370220968802

Cited by 17 publications

(11 citation statements)

References 135 publications

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“…It was noted that, in 80% of the cases (4 out of 5 samples), the high MAD values observed may be attributed to past or present smoking habits. Indeed, as already mentioned, many scholars have highlighted how these habits may influence the epigenetic modifications of individuals [ 4 , 10 , 11 , 12 ]. However, other factors, which are not currently under investigation, may be additional causes, which may have led to these results given the high complexity of epigenetic regulation.…”

Section: Discussionmentioning

confidence: 99%

“…Relative to this concept, as illustrated in the figure below ( Figure 1 ), defining the chronological and biological drivers of these DNA methylation clocks will need a deep analysis separately. The precise separation of these two elements due to particular sets of CpGs would result in more powerful specialized clocks and independent mechanistic research, especially in the case of legal measures of the human age [ 4 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Forensic Age Estimation through a DNA Methylation-Based Age Prediction Model in the Italian Population: A Pilot Study

Onofri

Delicati

Marcante

et al. 2023

IJMS

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DNA methylation is one of the epigenetic marks which has been studied intensively in recent years for age predicting purposes in the forensic area. In order to integrate age prediction into routine forensic workflow, the purpose of this study was to standardize and optimize a DNA methylation-based protocol tailored to the Italian context. A previously published protocol and age-predictive method was implemented for the analysis of 84 blood samples originating from Central Italy. The study here presented is based on the Single Base Extension method, considering five genes: ELOVL2, FHL2, KLF14, C1orf132, now identified as MIR29B2C, and TRIM59. The precise and specific steps consist of DNA extraction and quantification, bisulfite conversion, amplification of converted DNA, first purification, single base extension, second purification, capillary electrophoresis, and analysis of the results to train and test the tool. The prediction error obtained, expressed as mean absolute deviation, showed a value of 3.12 years in the training set and 3.01 years in the test set. Given that population-based differences in DNA methylation patterns have been previously reported in the literature, it would be useful to further improve the study implementing additional samples representative of the entire Italian population.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Forensic Age Estimation through a DNA Methylation-Based Age Prediction Model in the Italian Population: A Pilot Study

Onofri

Delicati

Marcante

et al. 2023

IJMS

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“…Forty-five years from the description of the biopsychosocial model of diseases, around 40 years after the first publication on comprehensive geriatric assessment (CGA), and over 20 years from the founding of the International Classification of Functioning, Disability and Health, no consensus and basically no definite clinical guideline exist on which CGA should be used in which setting and condition in older subjects. Indeed, recent evidence suggests that a scaled, quantifiable CGA measuring the domains of multimorbidity, polypharmacy, daily functions, cognition, and nutritional and social status can discriminate the power of promising aging clocks in terms of physiological relevance [89,90].…”

Section: Biomarkers Of Healthy Agingmentioning

confidence: 99%

Focus on senescence: Clinical significance and practical applications

Boccardi,

Orr,

Polidori

et al. 2024

J Intern Med

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The older population is increasing worldwide, and life expectancy is continuously rising, predominantly thanks to medical and technological progress. Healthspan refers to the number of years an individual can live in good health. From a gerontological viewpoint, the mission is to extend the life spent in good health, promoting well‐being and minimizing the impact of aging‐related diseases to slow the aging process. Biologically, aging is a malleable process characterized by an intra‐ and inter‐individual heterogeneous and dynamic balance between accumulating damage and repair mechanisms. Cellular senescence is a key component of this process, with senescent cells accumulating in different tissues and organs, leading to aging and age‐related disease susceptibility over time. Removing senescent cells from the body or slowing down the burden rate has been proposed as an efficient way to reduce age‐dependent deterioration. In animal models, senotherapeutic molecules can extend life expectancy and lifespan by either senolytic or senomorphic activity. Much research shows that dietary and physical activity‐driven lifestyle interventions protect against senescence. This narrative review aims to summarize the current knowledge on targeting senescent cells to reduce the risk of age‐related disease in animal models and their translational potential for humans. We focused on studies that have examined the potential role of senotherapeutics in slowing the aging process and modifying age‐related disease burdens. The review concludes with a general discussion of the mechanisms underlying this unique trajectory and its implications for future research.

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“…To date our understanding of parental care and ambient temperature in explaining post‐natal telomere dynamics at different thermoregulatory stages is limited. Descriptive studies of wild zebra finches Taeniopygia castanotis exposed to natural variation in ambient conditions have revealed positive correlations between ambient temperatures and another biomarker of aging, the DNA methylation rate (He et al, 2021; Sheldon et al, 2020), and of the latter with rates of telomere shortening (Sheldon et al, 2021). These correlative results suggest that thermal conditions during early life influence telomeres, but since temperature was not manipulated in that study (Sheldon et al, 2021), other factors may affect the results such as seasonal variation in reproductive investment, parental condition, or food availability.…”

Section: Introductionmentioning

confidence: 99%

“…To date our understanding of parental care and ambient temperature in explaining post-natal telomere dynamics at different thermoregulatory stages is limited. Descriptive studies of wild zebra finches Taeniopygia castanotis exposed to natural variation in ambient conditions have revealed positive correlations between ambient temperatures and another biomarker of aging, the DNA methylation rate (He et al, 2021;Sheldon et al, 2020), and of the latter with rates of telomere shortening (Sheldon et al, 2021).…”

mentioning

confidence: 99%

Effects of heat waves on telomere dynamics and parental brooding effort in nestlings of the zebra finch (Taeniopygia castanotis) transitioning from ectothermy to endothermy

et al. 2023

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Heat waves are predicted to be detrimental for organismal physiology with costs for survival that could be reflected in markers of biological state such as telomeres. Changes in early life telomere dynamics driven by thermal stress are of particular interest during the early post‐natal stages of altricial birds because nestlings quickly shift from being ectothermic to endothermic after hatching. Telomeres of ectothermic and endothermic organisms respond differently to environmental temperature, but few investigations within species that transition from ectothermy to endothermy are available. Also, ambient temperature influences parental brooding behaviour, which will alter the temperature experienced by offspring and thereby, potentially, their telomeres. We exposed zebra finch nestlings to experimental heat waves and compared their telomere dynamics to that of a control group at 5, 12 and 80 days of age that encapsulate the transition from the ectothermic to the endothermic thermoregulatory stage; we also recorded parental brooding, offspring sex, mass, growth rates, brood size and hatch order. Nestling mass showed an inverse relationship with telomere length, and nestlings exposed to heat waves showed lower telomere attrition during their first 12 days of life (ectothermic stage) compared to controls. Additionally, parents of heated broods reduced the time they spent brooding offspring (at 5 days old) compared to controls. Our results indicate that the effect of heat waves on telomere dynamics likely varies depending on age and thermoregulatory stage of the offspring in combination with parental brooding behaviour during growth.

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The use of DNA methylation clock in aging research

Cited by 17 publications

References 135 publications

Forensic Age Estimation through a DNA Methylation-Based Age Prediction Model in the Italian Population: A Pilot Study

Forensic Age Estimation through a DNA Methylation-Based Age Prediction Model in the Italian Population: A Pilot Study

Focus on senescence: Clinical significance and practical applications

Effects of heat waves on telomere dynamics and parental brooding effort in nestlings of the zebra finch (Taeniopygia castanotis) transitioning from ectothermy to endothermy

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