The use of DNA markers for genetic differentiation of common (Avena sativa L.) and naked oat (Avena nuda L.)

Abstract

The usefulness of genetic identification of varieties for seed quality analyses becomes important, when we suspect the presence of another variety, species or even genus, based on morphological seed traits in the purity analysis and germination test, With genetic analyses, the doubt about the authenticity of the naked oats (Avena nuda L.) variety 'Kamil' was successfully solved. There were atypical seeds with chaff among the samples of this variety, so it was not possible to confirm with certainty, whether these were seeds of the same species/variety or impurities of common oats (Avena sativa L.). Based on the seed phenotype of the 'Kamil' variety (two independent samples 284 and 285), four sub-samples were prepared (284AN, 284AS, 285AN, 285AS); AN label was the sub-sample with naked seeds and AS label was the sub-sample with chaffed seeds. In addition, another variety ('Noni') and four accessions of common oats (ACC378, ACC379, ACC380, ACC381) from the Slovene Plant Gene Bank were included as common oat standards. A total of 36 plant samples of Avena sp. were included in the genetic differentiation. Using a set of six highly informative SSR markers and the results of diversity parameters at individual loci and paired genetic comparisons, we were able to confirm that the analysed sub-samples belonged to the same variety, i.e. 'Kamil'. In addition, despite the morphological similarity, the chaffed seeds of naked oats variety 'Kamil' were sufficiently (genetically) different from the analysed variety and/or accessions of common oats.

Cultivars identification of oat (Avena sativa L.) seed via multispectral imaging analysis

“…Due to the continuous development and application of molecular marker technology in cultivar identification, molecular detection has been considered as an effective identification method with high precision ( Saccomanno et al., 2020 ). RFLP (restriction fragment length polymorphism), RAPD (randomly amplified polymorphic DNA), SSR (simple sequence repeat) and other molecular markers have been successfully applied in oat cultivars identification ( O’Donoughue et al., 1994 ; Paczos-Grzeda, 2004 ; Wight et al., 2010 ; Pipan et al., 2021 ). However, the lack of validated markers is one of the constraints of using molecular method ( Sakiyama et al., 2014 ), and it is generally conducted destructive.…”

Research on plant seeds recognition based on fine-grained image classification

“…In the Official Herbage and Turfgrass Seeds Testing Center, Ministry of Agriculture and Rural Affairs, China, there are nearly 2000 categories of seed identification tasks every year. Traditional methods of seed classification and identification include visual methods, chemical methods, biological methods, etc 3 , 4 . Because different seeds have different characteristics, such as shape and texture, which are important features to distinguish seed categories, physical observation method is the most direct and simplest method, but the realization of seed classification by observation method not only requires certain expertise but also has low efficiency and poor stability.…”

“…Traditional methods of seed classification and identification include visual methods, chemical methods, biological methods, etc. 3,4 Because different seeds have different characteristics, such as shape and texture, which are important features to distinguish seed categories, physical observation method is the most direct and simplest method, but the realization of seed classification by observation method not only requires certain expertise but also has low efficiency and poor stability. In addition, the chemical method is based on the different chemical and biological characteristics of different seeds, through these different characteristics can effectively identify their species and vitality.…”