The use of distilled water in the achievement of local hemostasis during surgery

Tillaart, Sabrina A.H.M. van den; Busard, M. P. H.; Trimbos, J. Baptist

doi:10.1007/s10397-008-0464-0

Cited by 11 publications

(3 citation statements)

References 18 publications

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“…Matrigel TM drops without organoids were infected with bacteria and treated with gentamicin as described above. Next, Matrigel TM drops containing or not organoids were washed with DMEM/12 at 37 °C and then lyzed in cold distilled H 2 O for 30 min on ice by osmotic shock [ 19 , 20 ]. Finally, the number of internalized and surviving bacteria at 4 h 30 pi was determined by plating appropriate dilution on Tryptic Soy Agar (TSA, BD Difco TM , Le Pont de Claix, France) and counted after overnight growth at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken

Lacroix-Lamandé,

Bernardi,

Pezier

et al. 2023

Vet Res

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Chicken infection with Salmonella Typhimurium is an important source of foodborne human diseases. Salmonella colonizes the avian intestinal tract and more particularly the caecum, without causing symptoms. This thus poses a challenge for the prevention of foodborne transmission. Until now, studies on the interaction of Salmonella with the avian gut intestine have been limited by the absence of in vitro intestinal culture models. Here, we established intestinal crypt‐derived chicken organoids to better decipher the impact of Salmonella intracellular replication on avian intestinal epithelium. Using a 3D organoid model, we observed a significantly higher replication rate of the intracellular bacteria in caecal organoids than in ileal organoids. Our model thus recreates intracellular environment, allowing Salmonella replication of avian epithelium according to the intestinal segment. Moreover, an inhibition of the cellular proliferation was observed in infected ileal and caecal organoids compared to uninfected organoids. This appears with a higher effect in ileal organoids, as well as a higher cytokine and signaling molecule response in infected ileal organoids at 3 h post-infection (hpi) than in caecal organoids that could explain the lower replication rate of Salmonella observed later at 24 hpi. To conclude, this study demonstrates that the 3D organoid is a model allowing to decipher the intracellular impact of Salmonella on the intestinal epithelium cell response and illustrates the importance of the gut segment used to purify stem cells and derive organoids to specifically study epithelial cell -Salmonella interaction.

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Section: Methodsmentioning

confidence: 99%

Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken

Lacroix-Lamandé,

Bernardi,

Pezier

et al. 2023

Vet Res

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“…The common reaction of OL with both NaOH and NaCl is shown in Figure e. It is worth mentioning that NaCl solution is isotonic with human blood at 154 mM concentration, which is very less to bring out any effect upon sensing, and the pH range for blood is typically between 7.35 and 7.45, with 7.40 being the average value, and no substantial decrease in fluorescence intensity is noticed in this range, confirming the suitability of the proposed Ch-OL/CQD nanocomposite sensing system for glucose sensing in real human blood samples.…”

Section: Resultsmentioning

confidence: 53%

Ex Vivo Glucose Detection in Human Blood Serums with Carbon Quantum Dot-Doped Oleic Acid-Treated Chitosan Nanocomposites

Patra,

Sahu,

Mahanty

et al. 2023

ACS Appl. Bio Mater.

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Herein, carbon quantum dot (CQD)-doped oleic acid (OL)-treated chitosan (Ch) nanocomposites (Ch-OL/CQD) are prepared by a simple solution technique for nonenzymatic ex vivo detection of glucose in human blood samples. From the architecture of the structure, it is observed that the agglomeration of CQD is restricted by OL-treated Ch polymeric chain, and simultaneously the inhibition in the entanglement of Ch-OL polymeric chains in the matrix is attained by the incorporation of CQD, thereby proving the high stability of the nanocomposite. In vitro detection of glucose is studied by the "Turn ON−OFF" fluorescence technique which is again evidenced by the shining core image of nanocomposites in HRTEM. A highly selective glucose sensing against interfering sugars due to the specific spatial arrangement of the hydroxyl groups of glucose, leading to prominent hydrogen-bonding interaction is established, with a very low limit of detection (LOD) of 1.51 μM, covering a wide linear domain from 0 to 10 4 μM, R 2 = 0.98. Moreover, the calculated glucose levels in real human blood serums by Ch-OL/CQD nanocomposites are compared with a commercial glucometer, with recovery percentages from 95.8 to 107.3%. The clinical potential is supported by studying the stimuli responsiveness of the nanocomposites as a function of pH and ionic strength, encouraging the operation of the sensor in a complex biological scenario. The present work may offer an opportunity for the monitoring of glucose in the blood for successful diabetes management.

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“…Distilled water or deionized water is commonly used for lavage to check homeostasis at the end of various operations [26]. The efficacy of pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma was proposed from the cytocidal effects of hypotonic shock on tumor cells [27].…”

Section: Discussionmentioning

confidence: 99%

The pH of water from various sources: an overview for recommendation for patients with atopic dermatitis

Kulthanan

Nuchkull

Varothai

2013

Asia Pacific Allergy

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BackgroundPatients with atopic dermatitis (AD) have increased susceptibility to irritants. Some patients have questions about types of water for bathing or skin cleansing.ObjectiveWe studied the pH of water from various sources to give an overview for physicians to recommend patients with AD.MethodsWater from various sources was collected for measurement of the pH using a pH meter and pH-indicator strips.ResultsBottled drinking still water had pH between 6.9 and 7.5 while the sparkling type had pH between 4.9 and 5.5. Water derived from home water filters had an approximate pH of 7.5 as same as tap water. Swimming pool water had had pH between 7.2 and 7.5 while seawater had a pH of 8. Normal saline and distilled water had pH of 5.4 and 5.7, respectively. Facial mineral water had pH between 7.5 and 8, while facial makeup removing water had an acidic pH.ConclusionNormal saline, distilled water, bottled sparkling water and facial makeup removing water had similar pH to that of normal skin of normal people. However, other factors including benefits of mineral substances in the water in terms of bacteriostatic and anti-inflammation should be considered in the selection of cleansing water.

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The use of distilled water in the achievement of local hemostasis during surgery

Cited by 11 publications

References 18 publications

Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken

Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken

Ex Vivo Glucose Detection in Human Blood Serums with Carbon Quantum Dot-Doped Oleic Acid-Treated Chitosan Nanocomposites

The pH of water from various sources: an overview for recommendation for patients with atopic dermatitis

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