The use of decalcified granulated homologous cortical bone matrix in the correction of diaphyseal bone defect

Abstract

In order to investigate an alternative way to correct diaphysial bone defects, granulated decalcified homologous cortical bone was used as a graft. Because of the suitable anatomic arrangement, the forearm of rabbits was chosen as an experimental model of bone defect. A 2 cm long bone cylinder was removed from the diaphysis of both radii preserving the periosteum. The artificial bone defect was filled with granular decalcified bone on the right side. The left side was used as control and kept empty or filled with undecalcified granular bone. The 18 animals were sacrificed in batches, 3,6, and 9 weeks after the operation. New bone formation was followed by X-rays, routine histology and incorporation of calcein blue, xylenol orange and tetracycline. In the decalcified granular bone grafts new bone formation was already detected at the first week and 9 weeks after the graft operation there was a well developed cylindric ossicle, in 89% of the cases. In some cases a medullary canal was present. No bone formation was found neither in the empty defects nor in the ones filled with undecalcified granular bone grafts.

A study of the mechanical strength of long bone defects treated with various bone autograft substitutes: An experimental investigation in the rabbit

“…cated use of other materials with osteoinductive properties, such as demineralized bone matrix (1,7,9,10,14,(17)(18)(19)22,23,25,27,28).…”

Resposta osteogênica sistêmica no estímulo medular ósseo

“…Some authors have been conducting experiments in an attempt to isolate substances produced on bodies, which would supposedly be correlated to bone formation. [8][9][10][11][12][13][14]22 In this experiment, three groups of animals were used: group A, submitted to ostectomy and to bone marrow remote stimulation by ablation, in order to assess the systemic osteogenic response after an intensive bone marrow regeneration process; group B, submitted to ostectomy and to bone marrow remote stimulation by placing a Kirschner wire inside the bone marrow in order to assess the systemic osteogenic response after an intensive bone marrow regeneration process , and; group C -control group -which was used for comparison. On the three groups, we performed blood depletion and used a garrote on the operated limb with an elastic band in order to reduce bleeding.…”

Bone formation enhanced by induction: Bone growth in titanium implants in rats

“…There have been numerous publications in which the authors have addressed the osteogenic capacity of various skeletal tissues and their combinations, or specially treated tissue derivatives (Burwell 1966 Lindholm et al 1982, Volpon et al 1982, Gupta & Tuli 1982, Cummine et al 1983, Wittbjer et al 1983. McDavid et al (1979) found that porous ceramic implants impregnated with bone mattow had bone formation within the pores.…”