The use of cultured tissue in studying developmental problems

Halperin, Walter

doi:10.1139/b73-232

Cited by 19 publications

(3 citation statements)

References 26 publications

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“…The retention of epigenetic factors that leads to failure of tissue to completely dedifferentiate could confine the organogenic potential of plant tissue (Halperin 1973). In the present investigation, extended exposure of stem microcuttings of WIMCO199 and L34 to liquid MS medium was found to be extremely beneficial as reported earlier (Chaturvedi et al 2004) in P. deltoides clones G3 and G48.…”

Section: Initial Establishment Of In Vitro Culturessupporting

confidence: 80%

High frequency direct plant regeneration from leaf, internode, and root segments of Eastern Cottonwood (Populus deltoides)

Yadav

Arora

Kumar

et al. 2009

Plant Biotechnol Rep

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Simple, reproducible, high frequency, improved plant regeneration protocol in Eastern Cottonwood (Populus deltoides) clones, WIMCO199 and L34, has been reported. Initially, aseptic cultures established from axillary buds of nodal segments from mature plus trees on MS liquid medium supplemented with 0.25 mg l -1 KIN and 0.25 mg l -1 IAA. Nodal and internodal segments were found to be extraprolific over shoot apices during course of aseptic culture establishment, while 0.25 mg l -1 KIN concentration played a stimulatory role in high frequency plant regeneration. Diverse explants, such as various leaf segments, internodes, and roots from in vitro raised cultures, were employed. Direct plant regeneration was at high frequency of 92% in internodes, 88% in leaf segments, and 43% in root segments. This led to the formation of multiple shoot clusters on established culture media with rapid proliferation rates. Many-fold enhanced shoot elongation and growth of the clusters could be achieved on liquid MS medium supplemented with borosilicate glass beads, which offer physical support for proliferating shoots leading to faster growth in comparison to semi-solid agar or direct liquid medium. SEM examination of initial cultures confirmed direct plant regeneration events without intervening calli. In vitro regenerated plants induced roots on half-strength MS medium with 0.15 mg l -1 IAA. Rooted 5-to 6-week-old in vitro regenerated plants were transferred into a transgenic greenhouse in pots containing 1:1 mixture of vermicompost and soil at 27 ± 2°C for hardening and acclimatization.14-to 15-week-old well-established hardened plants were transplanted to the field and grown to maturity. The mature in vitro raised poplar trees exhibited a high survival rate of 85%; 4-year-old healthy trees attained an average height of 8 m and an average trunk diameter of 25 cm and have performed well under field conditions. The regeneration protocol presented here will be very useful for undertaking genetic manipulation, providing a value addition to Eastern Cottonwood propagation in future.

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Section: Initial Establishment Of In Vitro Culturessupporting

confidence: 80%

High frequency direct plant regeneration from leaf, internode, and root segments of Eastern Cottonwood (Populus deltoides)

Yadav

Arora

Kumar

et al. 2009

Plant Biotechnol Rep

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show abstract

“…What this ability consists of and how some cells or tissues have or come to have it while others do not was left for speculation. Halperin (1973) proposed that the epigenetic factors, which comprise the differentiated state of the original explant tissues, may be lost during callus formation. In this classical view the callus cells are competent because they are undifferentiated, or 'dedifferentiated'.…”

Section: The Nature Of Competencementioning

confidence: 99%

Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture

Finstad

Brown

Joy

1993

Plant Cell Tiss Organ Cult

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Systematic manipulations of the culture protocol leading to somatic embryogenesis in alfalfa petiolederived callus were performed to study the competence phase during somatic embryogenesis in alfalfa. These demonstrated a requirement for the acquisition of competence prior to the induction of the embryogenesis pathway. Induction was triggered by a number of synthetic auxins including 2,4-dichlorophenoxyacetic acid (2,4-D). Competence could be acquired in the presence of these auxins as well as phenylacetic acid (PAA), an auxin that did not induce embryogenesis. Different degrees of competence were apparently acquired by exposure to 2,4-D and PAA. Some degree of competence was acquired in the absence of auxin treatment. The current understanding of the concept of competence is discussed.

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“…Especial mente na área de Fisiologia, esta técnica tem se mostrado vantajosa para o estudo de muitos problemas, os quais são mais facilmente estudados em fragmentos de tecidos isolados do que na planta intacta (BAJAJ, 1971). Isso se deve basicamente ao fato de que o cultivo "in vitro", seja de fragmentos de te cidos ou de células isoladas, permite vantajosamente um controle mais rigoroso das condições ambientais, nutricionais e hormonais (SIEVERT e HILDEBRANT, 1965;HANDRO, 1974), facili tando, desta forma, o estudo de certos problemas básicos dod~ senvolvimento vegetal, como citodiferenciação, organogenese e propagação vegetativa (HALPERIN, 1973). Foram fotografadas no Setor de Citogenética do Departamento de Genética da ESALQ/USP lâminas preparadas pelo método do micrótomo, utilizando corno material calos obtidos a partir de explantes irradiados de cana-de-açúcar.…”

Section: Introductionunclassified

Interação radiação gama x reguladores de crescimento no desenvolvimento de células e tecidos de cana-de-açúcar (>i<saccharum>/i< spp) in vitro

Ferreira¹

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The use of cultured tissue in studying developmental problems

Abstract: Selected aspects of studies dealing with xylogenesis, organ and embryo formation, and vegetative propagation in vitro are reviewed.

Cited by 19 publications

References 26 publications

High frequency direct plant regeneration from leaf, internode, and root segments of Eastern Cottonwood (Populus deltoides)

High frequency direct plant regeneration from leaf, internode, and root segments of Eastern Cottonwood (Populus deltoides)

Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture

Interação radiação gama x reguladores de crescimento no desenvolvimento de células e tecidos de cana-de-açúcar (>i<saccharum>/i< spp) in vitro

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