The Use of Cultured Bone Marrow Cells for Autologous Transplantation in Patients with Acute Myeloblastic Leukemia

Testa, NG; Coutinho, L. H.; Chang, James; Morgenstern, G. R.; Scarffe, J. H.; Dexter, T. M.

doi:10.1007/978-3-642-72624-8_16

Cited by 6 publications

References 6 publications

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Review: Tissue engineering: Reconstitution of human hematopoiesis ex vivo

Koller

Palsson

1993

Biotech & Bioengineering

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The reconstruction of functioning human tissues ex vivo is becoming an important part of biotechnology. There are compelling scientific, clinical, and biotechnological reasons for fully or partially reconstituting human tissues such as skin, bone marrow, and liver ex vivo. In particular, bone marrow is a tissue of much importance, and there are significant societal and health benefits derived from a successfully constructed ex vivo hematopoietic system. In this article, we review the current status of this effort. The topics covered include the current understanding of the biology of human hematopoiesis, the motivation for reconstructing it ex vivo, the current state of ex vivo human hematopoietic cultures, the development of important metrics to judge culture performance, and an approach based on in vivo mimetics to accomplish this goal. We discuss some applications of functional ex vivo hematopoietic cultures and the biological and engineering challenges that face research in this area.

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Review: Tissue engineering: Reconstitution of human hematopoiesis ex vivo

Koller

Palsson

1993

Biotech & Bioengineering

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Regulation of Haemopoietic Cell Development

Testa

Dexter

Allen

1991

Pharmaceutical Applications of Cell and Tissue Culture to Drug Transport

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Growth of normal versus leukemic bone marrow cells in long term culture from acute lymphoblastic and myeloblastic leukemias

Schirò

Coutinho

Will

et al. 1990

Blut

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The ability of the in vitro long-term bone marrow culture (LTBMC) system to impair the survival of leukemic cells and to enhance the growth of normal progenitors has been studied. Bone marrow cells from 19 acute lymphoblastic leukemia (ALL) and 30 acute myeloid leukemia (AML) patients at diagnosis were grown in LTBMC for 4-10 weeks. In half of the cases the leukemic population declined down to undetectable levels and was replaced by putative normal hemopoietic precursors, both in ALL and in AML. In the remaining cases, leukemic cells persisted throughout the culture time and few if any normal hemopoietic cells were detected. These data led us to extend to the lymphoid compartment the previous observation of decreasing leukemic myeloid blasts in LTBMC. The potential of such cultures as an in vitro purging system for autologous bone marrow transplantation in selected poor-prognosis lymphoid malignancies should be explored, as has been done for acute and chronic myeloid leukemias.

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The Use of Cultured Bone Marrow Cells for Autologous Transplantation in Patients with Acute Myeloblastic Leukemia

Cited by 6 publications

References 6 publications

Review: Tissue engineering: Reconstitution of human hematopoiesis ex vivo

Review: Tissue engineering: Reconstitution of human hematopoiesis ex vivo

Regulation of Haemopoietic Cell Development

Growth of normal versus leukemic bone marrow cells in long term culture from acute lymphoblastic and myeloblastic leukemias

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