The use of cryopreserved lymphocytes to standardize longitudinal studies of immune function in normal controls and patients

Jewett, Michael A.S.; Hansen, Julia; Gupta, Shashank; Cunningham‐Rundles, Susanna; Siegal, Frederick P.; Good, Robert A.; Dupont, Bo

doi:10.1016/0011-2240(75)90105-4

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While fresh cells should always be the first choice, for technical reasons it may sometimes be more practical to work with cryopreserved cells. Studies have shown that assessment of lymphocyte subsets and measurements of NK cell cytotoxicity and lymphocyte proliferation can be performed using cryopreserved cells (Jewett et al 1976;Fujiwara et al 1986;Whiteside et al 1990;Tollerud et al 1991;Allsopp et al 1998). However, although most studies did not observe significant differences between fresh and cryopreserved cells, the outcome for some individuals might differ significantly between fresh and cryopreserved cells for unknown reasons.…”

Section: Assessment Of Markersmentioning

confidence: 99%

See 1 more Smart Citation

Markers to measure immunomodulation in human nutrition intervention studies

et al. 2005

View full text Add to dashboard Cite

Normal functioning of the immune system is crucial to the health of man, and diet is one of the major exogenous factors modulating individual immunocompetence. Recently, nutrition research has focused on the role of foods or specific food components in enhancing immune system responsiveness to challenges and thereby improving health and reducing disease risks. Assessing diet-induced changes of immune function, however, requires a thorough methodological approach targeting a large spectrum of immune system parameters. Currently, no single marker is available to predict the outcome of a dietary intervention on the resistance to infection or to other immune system-related diseases. The present review summarises the immune function assays commonly used as markers in human intervention studies and evaluates their biological relevance (e.g. known correlation with clinically relevant endpoints), sensitivity (e.g. within-and between-subject variation), and practical feasibility. Based on these criteria markers were classified into three categories with high, medium or low suitability. Vaccine-specific serum antibody production, delayed-type hypersensitivity response, vaccine-specific or total secretory IgA in saliva and the response to attenuated pathogens, were classified as markers with high suitability. Markers with medium suitability include natural killer cell cytotoxicity, oxidative burst of phagocytes, lymphocyte proliferation and the cytokine pattern produced by activated immune cells. Since no single marker allows conclusions to be drawn about the modulation of the whole immune system, except for the clinical outcome of infection itself, combining markers with high and medium suitability is currently the best approach to measure immunomodulation in human nutrition intervention studies. It would be valuable to include several immune markers in addition to clinical outcome in future clinical trials in this area, as there is too little evidence that correlates markers with global health improvement.Immune function: Marker: Diet: Human studies: Infections Task and objectivesThe major function of the immune system is to protect the body against infectious diseases. The immune system can be divided into innate and adaptive immunity. The immune system operates at the systemic as well as at the local level, which includes the mucosal tissue such as in the upper airways and the gut. A fundamental characteristic of the immune system is that it involves multiple, functionally differing cell types, which permit a large variety of defence mechanisms. Assessing the status of the immune system and its functionality therefore requires a thorough methodological approach targeting a large spectrum of immune †A draft version of this review was extensively discussed with experts from the fields of nutrition, (clinical) immunology, mucosal immunology, gastroenterology and immunotoxicology during a Workshop, organised by the European branch of the International Life Sciences Institute (ILSI Europe), on 'Markers

show abstract

Section: Assessment Of Markersmentioning

confidence: 99%

“…The effect of cryopreservation on lymphocyte proliferation depends on the stimulus used to activate the lymphocytes. There is also a risk that subsets of cells can be selectively lost (Jewett et al 1976).…”

Section: Assessment Of Markersmentioning

confidence: 99%

Markers to measure immunomodulation in human nutrition intervention studies

et al. 2005

View full text Add to dashboard Cite

show abstract

Structure and functional alterations in lymphocytes induced by cryopreservation

Karpovitch

Ben-Basset

et al. 1980

Cryobiology

View full text Add to dashboard Cite

A Simple Method for the Cryopreservation of Human Lymphocytes at −80C

1977

View full text Add to dashboard Cite

A simple and inexpensive method for the freezing of human blood lymphocytes at -80 C is described. Lymphocytes were frozen in either polystyrene tubes or polyolefin bags. Optimal recovery of cells was obtained when 5% DMSO and 62% plasma were used as cryoprotectants. Viable cell recovery, as assessed by response to phyto hemagglutinin (PHA) and pokeweed mitogen (PWM), was greater than 80% after 60 days of storage. In addition, percentages of T and B lymphocytes were essentially unchanged. The excellent viable cell recovery in polyolefin bags suggests that it is possible to cryopreserve large numbers of lymphocytes in a single container.

show abstract

The use of cryopreserved lymphocytes to standardize longitudinal studies of immune function in normal controls and patients

Cited by 3 publications

References 0 publications

Markers to measure immunomodulation in human nutrition intervention studies

Markers to measure immunomodulation in human nutrition intervention studies

Structure and functional alterations in lymphocytes induced by cryopreservation

A Simple Method for the Cryopreservation of Human Lymphocytes at −80C

Contact Info

Product

Resources

About