The use of complex formation approach for spectroscopic analysis of certain oral hypoglycemic with Eosin Y

Ahmed, Hytham M.; El-Shabrawy, Yasser; Barseem, Aya; Belal, F.

doi:10.1016/j.microc.2019.104054

Cited by 4 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of medicinal substances have been quantified using this method [36][37][38][39]. Previously developed fluorometric methods for STG had disadvantages such as low sensitivity [14,15] or drastic coupling conditions with high-cost fluorogenic reagents [13]. Therefore, we developed a novel fluorometric method that would address these issues.…”

Section: Resultsmentioning

confidence: 99%

“…This article focuses on developing a new fluorometric method with advantages such as simplicity, adequate sensitivity, minimal solvent consumption, short analytical times, and selectivity [24–27]. Previously developed fluorometric methods for STG had disadvantages such as low sensitivity [14, 15], drastic coupling conditions, and expensive fluorogenic reagents [13]. Therefore, we developed a novel fluorometric method that addresses these issues.…”

Section: Introductionmentioning

confidence: 99%

“…STG assay alone or in combination with biguanide metformin using UV, first order, or colorimetry by application of the Hantzsch reaction was the spectrophotometric approach [6][7][8][9]. STG native fluorescence measurement [10][11][12], application of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) [13] or fluorescamine [14] as fluorogenic labeling, and complexation with xanthene-based dye [15] formed the basis for the spectrofluorimetric measurement of STG. Also, it was alleged that expensive equipment was used in research, including high-performance thin layer chromatography [16,17], highperformance liquid chromatography for STG and metformin in combination [18][19][20], and ultra-high-performance liquid chromatography [21].…”

mentioning

confidence: 99%

“…Previously developed fluorometric methods for STG had disadvantages such as low sensitivity [14,15], drastic coupling conditions, and expensive fluorogenic reagents [13]. Therefore, we developed a novel fluorometric method that addresses these issues.…”

mentioning

confidence: 99%

See 3 more Smart Citations

A new application of isoindole fluorophore derivative in sitagliptin antidiabetic medication assay: Application to dosage forms and biological fluid evaluation

et al. 2023

View full text Add to dashboard Cite

Dipeptidyl peptidase-4 enzyme suppressant is a unique category of oral antidiabetic medication. Sitagliptin (STG) is a perfect member of this category and is pharmaceutically marketed alone or in combination with metformin. Here, the ideal application of an isoindole derivative for STG assay was developed using a feasible, easy-to-use, economic, and affordable method. STG as an amino group donor can form a luminescent derivative: isoindole on interaction with o-phthalaldehyde and the existence of (2-mercaptoethanol) 0.02% (v/v) as a thiol group donor. Excitation (339.7 nm) and emission (434.6 nm) wavelengths were used to monitor the isoindole fluorophore yield; moreover, each experimental variable was carefully investigated and adjusted.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A new application of isoindole fluorophore derivative in sitagliptin antidiabetic medication assay: Application to dosage forms and biological fluid evaluation

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Fluorescence spectroscopy and its various applications have been reviewed over the years in several excellent publications [25,[43][44][45][46][47][48][49]. Almost all proteins have a natural fluorescence because of the following amino acids (Phe, Tyr, and Trp) [50,51].…”

Section: Fluorometric Investigationmentioning

confidence: 99%

Probing the potential toxicity of trimetazidine by characterizing its interaction with human serum albumin

Barseem

Belal

Mabrouk

et al. 2023

Methods Appl. Fluoresc.

Self Cite

View full text Add to dashboard Cite

Since the small molecular drugs pharmacodynamics and pharmacokinetics could be affected by human serum albumin (HSA) transport, we used spectroscopic and molecular docking studies to investigate the interaction between HSA and the angina-prevention drug trimetazidine (TMZ). As shown by synchronous fluorescence spectroscopy, this interaction affects the microenvironment confirmation around tyrosine residues. The site-competitive experiments showed that TMZ had an affinity toward subdomain III A (site II) of HSA. The enthalpy and entropy changes (ΔH and ΔS), which were 37.75 and 0.197 K J mol-1, respectively, showed that the predominant intermolecular interactions stabilizing the TMZ-HSA combination are hydrophobic forces. According to FTIR research, the interaction between HSA and TMZ caused polypeptide carbonyl-hydrogen bonds to rearrange. Additionally, the HSA esterase enzyme activity was assessed, which revealed a decrease in activity with TMZ. Docking analysis confirmed the site-competitive experiments and thermodynamic results. Also, it showed that TMZ has strong affinity for binding to HSA and inserted into HSA subdomain III A (site II) with score of -12.65. This study demonstrated that TMZ interacted with HSA, and the structure and function of HSA were influenced by TMZ. This study could aid in understanding the pharmacokinetics of TMZ and provide basic data for safe use.

show abstract

A unique application of pentane-2,4-dione as a fluorogenic probe of dipeptidyl peptidase-4 enzyme inhibitor medicament assay in pharmaceutical and biological matrices>

El Hamd,

Mohammed,

Mahdi

et al. 2023

Chem. Pap.

View full text Add to dashboard Cite

The use of complex formation approach for spectroscopic analysis of certain oral hypoglycemic with Eosin Y

Cited by 4 publications

References 26 publications

A new application of isoindole fluorophore derivative in sitagliptin antidiabetic medication assay: Application to dosage forms and biological fluid evaluation

A new application of isoindole fluorophore derivative in sitagliptin antidiabetic medication assay: Application to dosage forms and biological fluid evaluation

Probing the potential toxicity of trimetazidine by characterizing its interaction with human serum albumin

A unique application of pentane-2,4-dione as a fluorogenic probe of dipeptidyl peptidase-4 enzyme inhibitor medicament assay in pharmaceutical and biological matrices>

Contact Info

Product

Resources

About