“…Briefly, a culture grown ovemight in TSB was diluted with TSB to give approximately 105 to 106 colony-forming units per ml and incubated with different antibiotics in a water bath at 37 C. The final concentrations of antibiotics were as follows: streptomycin, 10 4g/ml; gentamicin, 4 jig/ml; vancomycin, 5 ,ug/ml; vancomycin, 10 1g/ml; vancomycin, 10 Ag/ml, with gentamicin, 4 gg/ml; vancomycin, 5 gg/ml, with gentamicin, 4 Ag/ml; vancomycin, 10 ,g/ml, with streptomycin, 10 ,g/ml; and vancomycin, 5 jig/ml, with streptomycin, 10 1sg/ml. At 6,24, and 48 h of incubation, viable colony-forming units were enumerated by the pour-plate technique with the use of TSA. Colonies on TSA were counted after incubation at 37 C for 48 h.…”