THE USE OF COLLOIDAL GOLD FOR STUDIES OF INTRACELLULAR EXCHANGES IN THE AMEBA <i>CHAOS CHAOS </i>

Feldherr, Carl M.; Marshall, John M.

doi:10.1083/jcb.12.3.640

Cited by 37 publications

(16 citation statements)

References 3 publications

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“…Colloidal gold was first used in 1962 for studies of intracellular exchange in amoeba (Chaos chaos) (Feldherr and Marshall, 1962), but its first applica tion as a specific marker for transmission electron microscopy was reported by Faulk and Taylor (1971). It was then introduced as a marker for scanning electron microscopy in 1975 (Horisberger et al, 1975) and for fluorescence microscopy (Horisberger and Vonlanthen, 1979).…”

Section: Colloidal Goldmentioning

confidence: 99%

Preparation and Application of Lectin–Gold Complexes

Benhamou

1989

Colloidal Gold

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Section: Colloidal Goldmentioning

confidence: 99%

Preparation and Application of Lectin–Gold Complexes

Benhamou

1989

Colloidal Gold

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“…This enzyme-labelled antibody technique was the second milestone in immunolabelling for electron microscopy. Feldherr & Marshall (1962) introduced colloidal gold particles as tracers for electron microscopy. Nine years later, colloidal gold was applied as a specific marker for antisera by Faulk & Taylor (1971), and by Romano et al, (1974) for isolated antibodies.…”

Section: Methodsmentioning

confidence: 99%

Immunoelectron microscopy of human spermatozoa: Immunelektronenmikroskopisch Untersuchung menschlicher Spermien

et al. 2009

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Summary A number of immunocytochemical methods using various markers for electron microscopy have been developed in recent years. The immunogold technique has been especially effective in histotopochemical studies. The value of this technique for demonstrating sperm antigens results from the high electron density of gold, which makes it easily detectable under the electron microscope. The high resolution of the electron microscope permits precise localization of immunologic reactions in the sperm cell. Light microscopy findings can thus be elucidated. We tested a number of monoclonal antibodies that react with sperm antigens. Of three techniques for preparing the spermatozoa, the pre‐embedding method and marking of cryoultra‐microtome sections proved best. Zusammenfassung Eine Reihe immuncytochemischer Methoden mit verschiedenen Markern wurde in den letzten Jahren für das Elektronenmikroskop entwickelt und beschrieben. Für histotopochemische Untersuchungen hat sich insbesondere die Immunogoldtechnik durchgesetzt. Der Vorteil der Methodik auch für den Nachweis von Spermienantigenen beruht auf der hohen Elektronendichte des Goldes und der damit verbundenen einfachen Detektion im Elektronenmikroskop. Die große Auflösung im Elektronenmikroskop gestattet eine genaue Lokalisation der immunologischen Reaktionsorte im Spermium und kann somit lichtmikroskopische Befunde präzisieren. Eine Reihe monoklonaler Anti‐körper, die mit Spermienantigenen reagieren, wurden getestet. Von drei Spermienpräpara‐tionsverfahren bewährten sich vor allem die Pre‐embedding‐Technik und die Markierung von Cryoultramikrotom‐Schnitten.

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“…To avoid these problems, electrondense molecules (such as ferritin or imferon) and metallic nanoparticles have been used widely to measure intracellular distances in combination with EM. These techniques were pioneered by CM Feldherr in 1962 [62][63][64][65][66]. Unfortunately, his research focused only on the nucleus and mitochondria of non-muscle cells.…”

Section: Nano-objects In Biomedical Researchmentioning

confidence: 99%

Delivery of nano-objects to functional Sub-domains of healthy and failing Cardiac myocytes

Lukyanenko

2007

Nanomedicine

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Cardiovascular disease, including heart failure, is one of the leading causes of mortality in the world. Delivery of nano-objects as carriers for markers, drugs or therapeutic genes to cellular organelles has the potential to sharply increase the efficiency of diagnostic and treatment protocols for heart failure. However, cardiac cells present special problems to the delivery of nano-objects, and the number of papers devoted to this important area is remarkably small. The present review discusses fundamental aspects, problems and perspectives in the delivery of nano-objects to functional sub-domains of failing cardiomyocytes. What size nano-objects can reach cellular sub-domains in failing hearts? What are the mechanisms for their permeation through the sarcolemma? How can we improve the delivery of nano-objects to the sub-domains? Answering these questions is fundamental to identifying cellular targets within the failing heart and the development of nanocarriers for heart-failure therapy at the cellular level.

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THE USE OF COLLOIDAL GOLD FOR STUDIES OF INTRACELLULAR EXCHANGES IN THE AMEBA CHAOS CHAOS

Cited by 37 publications

References 3 publications

Preparation and Application of Lectin–Gold Complexes

Preparation and Application of Lectin–Gold Complexes

Immunoelectron microscopy of human spermatozoa: Immunelektronenmikroskopisch Untersuchung menschlicher Spermien

Delivery of nano-objects to functional Sub-domains of healthy and failing Cardiac myocytes

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