The use of chondrogide membrane in autologous chondrocyte implantation

Haddo, Omar; Mahroof, S.; Higgs, Deborah; David, L.; Pringle, J.; Bayliss, Michael T.; Cannon, Steve; Briggs, Timothy

doi:10.1016/s0968-0160(03)00041-3

Cited by 191 publications

(150 citation statements)

References 12 publications

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“…Moreover, the periosteum exerts potential immunogenic effects (26). Therefore, some attempts were made to replace the periosteum using different resorbable scaffolds (27)(28)(29)(30)(31)(32)(33). In our model, the chondrospheres presumably adhered to the cartilage surface border by surface tension forces.…”

Section: Discussionmentioning

confidence: 99%

Long-term effects of chondrospheres on cartilage lesions in an autologous chondrocyte implantation model as investigated in the SCID mouse model

Schubert

Anders²,

Neumann³

et al. 2009

Int J Mol Med

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Abstract. Microtraumata often lead to articular cartilage lesions. Due to the bradytrophic character of hyaline cartilage, these lesions are hardly repaired by the organism. Autologous chondrocyte implantation (ACI) was established for restoring isolated structural cartilage defects in knee joints. However, results are not always convincing. Human chondrocytes from patients undergoing total knee arthroplasty were cultured in monolayer followed by condensing single chondrocytes to spheroids (chondrospheres ® ). The integrative capacity of chondrospheres was examined by implanting them into lesions in human articular cartilage specimens and co-implanting them into SCID mice. Mice were sacrificed after 4, 12 and 24 weeks. HE and safranin O staining as well as immunohistochemistry using anti-S100, anti-collagen I and II antibodies were performed and analyzed using semiquantitative scores. Integration of the chondrospheres with the (native) cartilage matrix was analyzed by determining the percentage of adhering surface. With respect to long-term stability, the chondrocytes within chondrospheres showed a typical chondrocytic morphology. Immunohistochemically, a high collagen II production was detected. Over a time period of 24 weeks, an increasing content of collagen type II, glycosaminoglycans and collagenous fibers were found. Importantly, the newly synthesized cartilaginous matrix integrated continuously with the native cartilage lesion border. In conclusion, the presented data demonstrate that chondrospheres are able to restore and conserve their phenotype for at least 24 weeks under in vivo conditions. Moreover, chondrospheres adhere to full-thickness cartilage defects and appear to produce a cartilaginous extracellular matrix which fuses with native cartilage thus generating an autologous cartilage-like repair tissue.

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Section: Discussionmentioning

confidence: 99%

Long-term effects of chondrospheres on cartilage lesions in an autologous chondrocyte implantation model as investigated in the SCID mouse model

Schubert

Anders²,

Neumann³

et al. 2009

Int J Mol Med

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“…As an alternative to periosteal flaps, collagen membranes, e.g. ChondroGide  (Geistlich Biomaterials, Wolhusen, Switzerland), are used during the second generation of ACT, resulting in satisfactory repair without hypertrophic development at a 1 year post-surgery arthroscopy (Haddo et al, 2004).…”

Section: Autologous Chondrocyte Transplantation and Matrix-associatedmentioning

confidence: 99%

State of the art and future perspectives of articular cartilage regeneration: a focus on adipose-derived stem cells and platelet-derived products

Hildner

Albrecht

Gabriel

et al. 2011

J Tissue Eng Regen Med

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Trauma, malposition and age-related degeneration of articular cartilage often result in severe lesions that do not heal spontaneously. Many efforts over the last centuries have been undertaken to support cartilage healing, with approaches ranging from symptomatic treatment to structural cartilage regeneration. Microfracture and matrix-associated autologous chondrocyte transplantation (MACT) can be regarded as one of the most effective techniques available today to treat traumatic cartilage defects. Research is focused on the development of new biomaterials, which are intended to provide optimized physical and biochemical conditions for cell proliferation and cartilage synthesis. New attempts have also been undertaken to replace chondrocytes with cells that are more easily available and cause less donor site morbidity, e.g. adipose derived stem cells (ASC). The number of in vitro studies on adult stem cells has rapidly increased during the last decade, indicating that many variables have yet to be optimized to direct stem cells towards the desired lineage. The present review gives an overview of the difficulties of cartilage repair and current cartilage repair techniques. Moreover, it reviews new fields of cartilage tissue engineering, including stem cells, co-cultures and platelet-rich plasma (PRP).

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“…The outcome of these chondrocyte-based techniques is generally quite good (Minas, 2001;Peterson et al, 2000) but in many cases results in the formation of non-hyaline cartilage repair tissue with inferior mechanical properties and limited durability (Pelttari et al, 2009). ACI has several technical limitations: a) obtaining cartilage explants requires an additional surgical intervention, adding to the articular cartilage damage that increases the osteoarthritic process (Marcacci et al, 2002); b) in vitro chondrocyte proliferation must be limited because the capacity to produce stable cartilage in vivo is gradually reduced when cell divisions are increased (Dell´Accio et al, 2001); c) aging reduces the cellular density of the cartilage, which impacts chondrocyte proliferation capacity in vitro (Menche et al, 1998) and the chondrogenic potential of the periosteum (O´Driscoll & Fitzsimmons, 2001), d) cell culture procedures take too long (3 to 6 weeks) and increase the risk of contamination, e) risk of leakage of transplanted chondrocytes from the cartilage defects, f) the effects of gravity causing the chondrocytes to sink to the dependent side of the defect, resulting in an unequal distribution of cells that hampers the homogenous regeneration of the cartilage (Díaz-Prado et al, 2010c;Sohn et al, 2002), g) not the least the reacquisition of phenotypes of dedifferentiated chondrocytes in a monolayer culture (Kimura et al, 1984;Benya & Shaffer, 1982) and h) hypertrophy of tissue (Steinwachs & Kreuz, 2007;Haddo et al, 2004). The use of periosteum membrane poses constraints and the need for wide surgical incision, hypertrophy of the periosteum peripheral implant and its potential for ectopic calcification.…”

Section: Autologous Chondrocyte Implantationmentioning

confidence: 99%

“…The use of periosteum membrane poses constraints and the need for wide surgical incision, hypertrophy of the periosteum peripheral implant and its potential for ectopic calcification. As an alternative it has been proposed the use of a membrane collagen type I/III (Haddo et al, 2004;Krishnan et al, 2006;Robertson et al, 2007). The use of both kinds of membranes shows no significant differences in the clinical assessment, although arthroscopic analysis showed that after implantation of periosteum a substantial number of patients required a cleanup of the peripheral hypertrophy (Gooding et al, 2006).…”

Section: Autologous Chondrocyte Implantationmentioning

confidence: 99%