Abstract:Nowadays, biopolymers as intelligent and active biopolymer systems in the food and pharmaceutical industry are of considerable interest in their use. With this association in view, biopolymers such as chitosan, alginate, pectin, cellulose, agarose, guar gum, agar, carrageenan, gelatin, dextran, xanthan, and other polymers have received significant attention in recent years due to their abundance and natural availability. Furthermore, their versatile properties such as non-toxicity, biocompatibility, biodegrada… Show more
“…For example, Cheng et al [49] employed a K. pneumoniae strain M5al in batch cultivation at a bioreactor level and achieved 18 g/L PDO and up to 5 g/L of BDO after 18 h of cultivation, while the pH was maintained at 6.8 through the automatic addition of NaOH, and the starting substrate concentration was 40 g/L of glycerol. Da PDO is a three-carbon diol with an important contribution in ecological materials division (e.g., biopolymers, polyesters, composites, coatings), while BDO is a four-carbon diol with a major role in the industry of polymers [34,35,48]. Considering the results obtained for PDO and BDO (Table 1, Figure 1), these were similar to those reported in the literature.…”
Section: Resultssupporting
confidence: 87%
“…A higher pH tolerance (e.g., 8) was related to the genetically modified strains, which were optimized for an elevated metabolite production such as PDO [47], while a lower pH level inhibits cell division [46]. PDO is a three-carbon diol with an important contribution in ecological materials division (e.g., biopolymers, polyesters, composites, coatings), while BDO is a four-carbon diol with a major role in the industry of polymers [34,35,48]. Considering the results obtained for PDO and BDO (Table 1, Figure 1), these were similar to those reported in the literature.…”
Despite being a well-known human pathogen, Klebsiella pneumoniae plays a significant role in the biotechnology field, being considered as a microbial cell factory in terms of valuable chemical biosynthesis. In this work, Klebsiella pneumoniae DSMZ 2026 was investigated for its potential to biosynthesize 1,3-propanediol (PDO) and 2,3-butanediol (BDO) during batch fermentation under controlled and uncontrolled pH levels. The bacterial strain was cultivated at a bioreactor level, and it was inoculated in 2 L of specific mineral broth containing 50 g/L of glycerol as the main carbon source. The process was conducted under anaerobic conditions at 37 • C and 180 RPM (rotations per minute) for 24 h. The effect of pH oscillation on the biosynthesis of PDO and BDO was investigated. Samples were taken every 3 h and specific tests were performed: pH measurement, main substrate consumption, PDO and BDO production. The cell morphology was analyzed on both solid and liquid media. After 24 h of cultivation, the maximum concentrations of PDO and BDO were 28.63 ± 2.20 g/L and 18.10 ± 1.10 g/L when the pH value was maintained at 7. Decreased concentrations of PDO and BDO were achieved (11.08 ± 0.14 g/L and 7.35 ± 0.00 g/L, respectively) when the pH level was not maintained at constant values. Moreover, it was identified the presence of other metabolites (lactic, citric, and succinic acids) in the cultivation media at the beginning of the process, after 12 h and 24 h of cultivation.
“…For example, Cheng et al [49] employed a K. pneumoniae strain M5al in batch cultivation at a bioreactor level and achieved 18 g/L PDO and up to 5 g/L of BDO after 18 h of cultivation, while the pH was maintained at 6.8 through the automatic addition of NaOH, and the starting substrate concentration was 40 g/L of glycerol. Da PDO is a three-carbon diol with an important contribution in ecological materials division (e.g., biopolymers, polyesters, composites, coatings), while BDO is a four-carbon diol with a major role in the industry of polymers [34,35,48]. Considering the results obtained for PDO and BDO (Table 1, Figure 1), these were similar to those reported in the literature.…”
Section: Resultssupporting
confidence: 87%
“…A higher pH tolerance (e.g., 8) was related to the genetically modified strains, which were optimized for an elevated metabolite production such as PDO [47], while a lower pH level inhibits cell division [46]. PDO is a three-carbon diol with an important contribution in ecological materials division (e.g., biopolymers, polyesters, composites, coatings), while BDO is a four-carbon diol with a major role in the industry of polymers [34,35,48]. Considering the results obtained for PDO and BDO (Table 1, Figure 1), these were similar to those reported in the literature.…”
Despite being a well-known human pathogen, Klebsiella pneumoniae plays a significant role in the biotechnology field, being considered as a microbial cell factory in terms of valuable chemical biosynthesis. In this work, Klebsiella pneumoniae DSMZ 2026 was investigated for its potential to biosynthesize 1,3-propanediol (PDO) and 2,3-butanediol (BDO) during batch fermentation under controlled and uncontrolled pH levels. The bacterial strain was cultivated at a bioreactor level, and it was inoculated in 2 L of specific mineral broth containing 50 g/L of glycerol as the main carbon source. The process was conducted under anaerobic conditions at 37 • C and 180 RPM (rotations per minute) for 24 h. The effect of pH oscillation on the biosynthesis of PDO and BDO was investigated. Samples were taken every 3 h and specific tests were performed: pH measurement, main substrate consumption, PDO and BDO production. The cell morphology was analyzed on both solid and liquid media. After 24 h of cultivation, the maximum concentrations of PDO and BDO were 28.63 ± 2.20 g/L and 18.10 ± 1.10 g/L when the pH value was maintained at 7. Decreased concentrations of PDO and BDO were achieved (11.08 ± 0.14 g/L and 7.35 ± 0.00 g/L, respectively) when the pH level was not maintained at constant values. Moreover, it was identified the presence of other metabolites (lactic, citric, and succinic acids) in the cultivation media at the beginning of the process, after 12 h and 24 h of cultivation.
“…There is an increasing interest in the use of biomass-derived polymers and the study of techniques for the development of biodegradable packaging, an alternative to the use of polymers from non-renewable sources for use in bioplastic packaging [17,18]. Moreover, interest in natural substances that can be used as process additives in polymers is increasing [19,20].…”
In the current work the physicochemical features of poly(vinyl alcohol) (PVOH) biofilms, enriched with eco-friendly polyols and with carotenoid-rich extracts, were investigated. The polyols, such as glycerol (Gly), 1,3-propanediol (PDO), and 2,3-butanediol (BDO) were used as plasticizers and the tomato-based pigments (TP) as coloring agents. The outcomes showed that β-carotene was the major carotenoid in the TP (1.605 mg β-carotene/100 DW), which imprinted the orange color to the biofilms. The flow behavior indicated that with the increase of shear rate the viscosity of biofilm solutions also increased until 50 s−1, reaching values at 37 °C of approximately 9 ± 0.5 mPa·s for PVOH, and for PVOH+TP, 14 ± 0.5 mPa·s in combination with Gly, PDO, and BDO. The weight, thickness, and density of samples increased with the addition of polyols and TP. Biofilms with TP had lower transparency values compared with control biofilms (without vegetal pigments). The presence of BDO, especially, but also of PDO and glycerol in biofilms created strong bonds within the PVOH matrix by increasing their mechanical resistance. The novelty of the present approach relies on the replacement of synthetic colorants with natural pigments derived from agro-industrial by-products, and the use of a combination of biodegradable polymers and polyols, as an integrated solution for packaging application in the bioplastic industry.
“…For example, ultrasound-assisted extraction (UAE) [31,32] represents a novel and low-cost technique that can improve the bioactive compound extraction rate and efficiency. UAE can be applied for both the extraction of interest compounds and for their encapsulation by different biopolymers [33][34][35][36][37]. There are different thermal processes (e.g., steaming, pressured steam-heating, drum drying, roasting, and microwave heating), largely applied to increase the palatability, stability, and safety of cereal-based food products.…”
The aim of the present paper was to identify the major polyphenolic compounds and investigate the antioxidant, antimutagenic, and antimicrobial activities of industrially-derived cereal byproducts-wheat bran (WB) and oat bran (OB)-before (fresh) and after thermal processing (TP) (10 min, 80 • C), coupled with ultrasound-asssited extraction. The results showed that the thermal process improved the total phenolic content of WB by +22.49%, and of OB with +25.84%. After the TP, the phenolic concentration showed a significant relative percentage increase in the case of WB (ferulic acid +39.18%, vanillic acid +95.68%, apigenin-glucoside +71.96%, p-coumaric acid +71.91) and of OB (avenanthramide 2c +52.17%, dihydroxybenzoic acids +38.55%). The best antioxidant capacity was registered by OBTP followed by WBTP. The strongest antimicrobial inhibition was attributed to the WBTP sample. Both thermally processed matrices had strong antimutagenic activity toward S. typhimurium TA100. This thermal processing was tested on bran based on its practical application within the food industry, considering the design of different cereal byproducts derived from functional foods and nutraceuticals.
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