The use of bis(mercaptoacetato-S,O)hydroxoiron(III) complex for the determination of hydroxamates

Arnold, Lee D.; Viswanatha, T.

doi:10.1016/0165-022x(83)90005-2

Cited by 23 publications

(6 citation statements)

References 31 publications

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“…Upon removal of Fe(III) from the purple dye complex, a decrease in absorbance is observed at 532 nm. Assay solutions were prepared as described by Arnold and Viswanatha (1983) (chemicals from Sigma). An amount of 1 ml of assay reagent was added to 2 ml each of culture fluid, blank and deferoxamine mesylate standard.…”

Section: Siderophore Assays and High-performance Liquid Chromatographymentioning

confidence: 99%

Microbial leaching of uranium and other trace elements from shale mine tailings at Ranstad

et al. 2004

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Section: Siderophore Assays and High-performance Liquid Chromatographymentioning

confidence: 99%

Microbial leaching of uranium and other trace elements from shale mine tailings at Ranstad

et al. 2004

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“…Possible catechol siderophore production was assessed by the colorimetric assays of Arnow (1937) and Rioux et al (1983), using 2,3-dihydroxybenzoic acid (2,3-DHBA) as standard. Possible hydroxamate siderophore production was assessed in lyophilized 10-fold concentrated culture supernatant by the colorimetric assay of Csaky (1948) and in unconcentrated culture supernatant by the assays of Atkin et al (1970) and Arnold & Viswanatha (1983), using hydroxylamine monohydrochloride and desferrioxamine mesylate (Desferal ; Ciba-Geigy) as standards. Crude siderophore preparations were analysed by paper and thin-layer chromatography (TLC).…”

Section: Methodsmentioning

confidence: 99%

Siderophore production by Aeromonas salmonicida

Hirst

Hastings

Ellis

1991

Journal of General Microbiology

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Growth under conditions of iron-restriction and the production of siderophores was examined in 21 typical and 14 atypical strains of Aeromonas salmonicida. With the exception of one atypical strain, all strains grew and multiplied in the presence of the high-affinity iron chelators ethylenediamine di(o-hydroxyphenylacetic acid), a, a'-dipyridyl or transferrin. Chrome azurol S agar was used to screen bacterial strains growing under these conditions for the production of siderophores. Siderophore production was detected only in the typical strains. Siderophores were also detected in the iron-restricted culture supernatants of typical strains, where they were associated with an iron-binding activity. The siderophore was extracted from iron-restricted culture supernatant of one strain by adsorption onto an XAD-7 resin; it behaved as a 2,3-diphenol-catechol in several colorimetric assays. The results indicate that although both typical and atypical strains of A. safmunicida grow and multiply under conditions of iron-restriction, they use different iron-uptake mechanisms, siderophore-mediated and siderophoreindependent, respectively. In cross-feeding assays, growth of typical strains was stimulated only by homologous iron-restricted supernatant, suggesting strain differences in the siderophore produced. However, one strain produced a culture supernatant with growth-stimulating activity for other typical and also atypical strains.

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“…The effluent (containing only desferriaerobactin) was collected and dried under reduced pressure at 45°C. The residue was dissolved in 8 ml of water, and aliquots were used to determine aerobactin production by reaction with bis(mercaptoacetate)Fe(III) complex reagent (1).…”

mentioning

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Construction and biochemical characterization of recombinant cytoplasmic forms of the IucD protein (lysine:N6-hydroxylase) encoded by the pColV-K30 aerobactin gene cluster

et al. 1993

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The aerobactin gene cluster in pColV-K30 consists of five genes (iucABCD iutA); four of these (iucABCD) are involved in aerobactin biosynthesis, whereas the fifth one (iutA) encodes the ferriaerobactin outer membrane receptor. iucD encodes lysine:NM-hydroxylase, which catalyzes the first step in aerobactin biosynthesis. Regardless of the method used for cell rupture, we have consistently found that IucD remains membrane bound, and repeated efforts to achieve a purified and active soluble form of the enzyme have been unsuccessful. To circumvent this problem, we have constructed recombinant IucD proteins with modified amino termini by creating three in-frame gene fusions of IucD to the amino-terminal amino acids of the cytoplasmic enzyme 13-galactosidase. Two of these constructs resulted in the addition to the iucD coding region of a hydrophilic leader sequence of 13 and 30 amino acids. The other construct involved the deletion of the first 47 amino acids of the IucD amino terminus and the addition of 19 amino acids of the amino terminus of 1-galactosidase. Cells expressing any of the three recombinant IucD forms were found to produce soluble N6-hydroxylysine. One of these proteins, lucD439, was purified to homogeneity from the soluble fraction of the cell lysates, and it was capable of participating in the biosynthesis of aerobactin, as determined in vitro by a cell-free system and in vivo by a cross-feeding bioassay. A medium ionic strength of 0.25 (250 mM NaCl) or higher was required to maintain the protein in a catalytically functional, tetrameric state. The enzyme was stringently specific with regard to its substrate and cofactor, L-lysine and flavin adenine dinucleotide, respectively. NADPH appeared to be the preferred electron donor used by IucD439 in the N hydroxylation process.

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The use of bis(mercaptoacetato-S,O)hydroxoiron(III) complex for the determination of hydroxamates

Cited by 23 publications

References 31 publications

Microbial leaching of uranium and other trace elements from shale mine tailings at Ranstad

Microbial leaching of uranium and other trace elements from shale mine tailings at Ranstad

Siderophore production by Aeromonas salmonicida

Construction and biochemical characterization of recombinant cytoplasmic forms of the IucD protein (lysine:N6-hydroxylase) encoded by the pColV-K30 aerobactin gene cluster

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