This study demonstrates the effects of impregnation of polyvinyl alcohol (PVA), styrene, and nanoclay on the compositional, mechanical, morphological, and thermal properties of bamboo and its nanocomposites (NCs). Design Expert software was used to optimize the materials and modulus of elasticity as well as the modulus of rupture of the composites. Models were obtained with R2 of 0.9999 and 0.998 for the modulus of elasticity (MOE) and the modulus of rupture (MOR), respectively. The MOE and MOR were found to increase significantly after the formation of NC that indicated improvement of mechanical properties of the bamboo NCs. The polymerization of PVA-co-styrene and dispersion of nanoclay were confirmed by the compositional analysis. The Xray diffraction analysis showed that the degree of crystallinity was slightly improved upon impregnation with PVA-co-styrene, while reduction in the hydroxyl groups was observed using the Fourier transform infrared. The scanning electron microscopy observation showed tightly filled cell cavities of the bamboo matrix. The thermal stability of the formed NCs were found to be slightly less stable than the raw bamboo (RB) with the differential scanning calorimetry showing low glass transition temperature and the thermogravimetric analysis showed lower decomposition temperatures for the NCs compared to RB as a result of plastic property of the styrene.