The use of bacterial cellulose as a basement membrane improves the plausibility of the static in vitro blood-brain barrier model

Bayır, Ece; Celtikoglu, M. Mert; Ürkmez, Aylin Şendemir

doi:10.1016/j.ijbiomac.2018.12.257

Cited by 14 publications

(4 citation statements)

References 40 publications

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“…After calibration, all compounds were passed through the model for 60 min (three replicates), and the permeability values were calculated according to the formula above. [ 43 ]…”

Section: Methodsmentioning

confidence: 99%

Novel multifunctional tacrine–donepezil hybrids against Alzheimer's disease: Design synthesis and bioactivity studies

Bayraktar,

Bartolini,

Bolognesi

et al. 2024

Archiv der Pharmazie

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A series of tacrine–donepezil hybrids were synthesized as potential multifunctional anti‐Alzheimer's disease (AD) compounds. For this purpose, tacrine and the benzylpiperidine moiety of donepezil were fused with a hydrazone group to achieve a small library of tacrine–donepezil hybrids. In agreement with the design, all compounds showed inhibitory activity toward both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with IC50 values in the low micromolar range. Kinetic studies on the most potent cholinesterase (ChE) inhibitors within the series showed a mixed‐type inhibition mechanism on both enzymes. Also, the docking studies indicated that the compounds inhibit ChEs by dual binding site (DBS) interactions. Notably, tacrine–donepezil hybrids also exhibited significant neuroprotection against H2O2‐induced cell death in a differentiated human neuroblastoma (SH‐SY5Y) cell line at concentrations close to their IC50 values on ChEs and showed high to medium blood–brain barrier (BBB) permeability on human cerebral microvascular endothelial cells (HBEC‐5i). Besides, the compounds do not cause remarkable toxicity in a human hepatocellular carcinoma cell line (HepG2) and SH‐SY5Y cells. Additionally, the compounds were predicted to also have good bioavailability. Among the tested compounds, H4, H16, H17, and H24 stand out with their biological profile. Taken together, the proposed novel tacrine–donepezil scaffold represents a promising starting point for the development of novel anti‐ChE multifunctional agents against AD.

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“…After calibration, all compounds were passed through the model for 60 min (three replicates), and the permeability values were calculated according to the formula above. [ 43 ]…”

Section: Methodsmentioning

confidence: 99%

Novel multifunctional tacrine–donepezil hybrids against Alzheimer's disease: Design synthesis and bioactivity studies

Bayraktar,

Bartolini,

Bolognesi

et al. 2024

Archiv der Pharmazie

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“…After removing HDMS, hydrogels were dried in a fume hood and observed with SEM after sputter-coated with 7 nm Au-Pd. 34…”

Section: Methodsmentioning

confidence: 99%

“…After removing HDMS, hydrogels were dried in a fume hood and observed with SEM after sputter-coated with 7 nm Au-Pd. 34 Transendothelial electrical resistance (TEER) measurement. The TEER values of the BBB models were measured with a volt/ohm meter (EVOM2, WPI, USA) and Ag/AgCl electrodes (STX2, WPI, USA) for 3 days.…”

Section: Solid Volumementioning

confidence: 99%

Development of a three-dimensional in vitro blood-brain barrier using the chitosan-alginate polyelectrolyte complex as the extracellular matrix

Bayır

2023

Journal of Bioactive and Compatible Polymers

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Polyelectrolyte complexes (PECs) consist of a spontaneous assembly of oppositely charged polysaccharides. PECs can be used to obtain a hydrogel tissue scaffold in tissue culture. In this study, it is aimed to use PEC as a blood-brain barrier (BBB) model scaffold. By mixing polycationic chitosan and polyanionic alginate solutions at a certain ratio it was obtained a 3D hydrogel scaffold and mimicked in vivo environment of the tissue. The PEC hydrogel scaffold’s chemical, physical, and mechanical characterizations were performed with FTIR, DSC, DMA, and Micro-CT analyses. In order to develop an in vitro BBB model, the human neuroblastoma cell line (SH-SY5Y) and mouse astrocyte cell line (C8-D1A) were mixed into a hydrogel, which is the abluminal side of the BBB. Human microvascular endothelial cells (HBEC-5i) were seeded on the hydrogel, and it was aimed to mimic the luminal side of the BBB. The characterization of the BBB model was determined by measuring the TEER, observation of the cell morphology with SEM, performing the permeability of Lucifer Yellow, and observation of tight junction proteins with immunofluorescence staining. As a result, HBEC-5i cells expressed tight junction proteins (ZO-1 and Claudin-5), showed TEER of 340 ± 22 Ω.cm2, and the Lucifer Yellow permeability of 7.4 × 10−7 ± 2.7 × 10−7 cm/s, which was suitable for use as an in vitro BBB model. Using a hydrogel PEC composed of chitosan and alginate as an extracellular matrix increased the direct interaction of endothelial cells, astrocytes, and neurons with each other and thus obtained a much less permeable model compared to other standard transwell models. Graphical abstract [Formula: see text]

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“…Aside from cells and biomolecules, the basement membrane (BM) has a critical role in the control of BBB permeability. This membrane connects cells, regulates intercellular communication, and manages the barrier function by interacting with extracellular matrix (ECM) proteins [ 56 ]. BM is made up of several molecules such as collagen, nidogen, laminin, sulfate, proteoglycans, and other glycoproteins [ 57 , 58 ].…”

Section: Bbb Structurementioning

confidence: 99%

Blood-Brain Barrier Overview: Structural and Functional Correlation

Alahmari

2021

Neural Plasticity

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The blood-brain barrier (BBB) is a semipermeable and extremely selective system in the central nervous system of most vertebrates, that separates blood from the brain’s extracellular fluid. It plays a vital role in regulating the transport of necessary materials for brain function, furthermore, protecting it from foreign substances in the blood that could damage it. In this review, we searched in Google Scholar, Pubmed, Web of Science, and Saudi Digital Library for the various cells and components that support the development and function of this barrier, as well as the different pathways to transport the various molecules between blood and the brain. We also discussed the aspects that lead to BBB dysfunction and its neuropathological consequences, with the identification of some of the most important biomarkers that might be used as a biomarker to predict the BBB disturbances. This comprehensive overview of BBB will pave the way for future studies to focus on developing more specific targeting systems in material delivery as a future approach that assists in combinatorial therapy or nanotherapy to destroy or modify this barrier in pathological conditions such as brain tumors and brain stem cell carcinomas.

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The use of bacterial cellulose as a basement membrane improves the plausibility of the static in vitro blood-brain barrier model

Cited by 14 publications

References 40 publications

Novel multifunctional tacrine–donepezil hybrids against Alzheimer's disease: Design synthesis and bioactivity studies

Novel multifunctional tacrine–donepezil hybrids against Alzheimer's disease: Design synthesis and bioactivity studies

Development of a three-dimensional in vitro blood-brain barrier using the chitosan-alginate polyelectrolyte complex as the extracellular matrix

Blood-Brain Barrier Overview: Structural and Functional Correlation

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