The Use of Bacterial Alkaline Phosphatase Assay for Rapid Monitoring of Bacterial Counts on Spinach

Park, E.‐J.; Kang, Dong‐Hyun

doi:10.1111/j.1750-3841.2008.00763.x

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…Protein was removed from the extracts with phenol/chloroform (1:1 ( v / v )), followed by three successive extractions with chloroform. The polyP content was determined spectrophotometrically using a Beckman DU-64 spectrophotometer [51,53]. The resulting polyP yield was 70 µg/g bacteria (wet weight), following the established determination method [52].…”

Section: Methodsmentioning

confidence: 99%

Transformation of Construction Cement to a Self-Healing Hybrid Binder

Müller

Tolba

Wang

et al. 2019

IJMS

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A new biomimetic strategy to im prove the self-healing properties of Portland cement is presented that is based on the application of the biogenic inorganic polymer polyphosphate (polyP), which is used as a cement admixture. The data show that synthetic linear polyp, with an average chain length of 40, as well as natural long-chain polyP isolated from soil bacteria, has the ability to support self-healing of this construction material. Furthermore, polyP, used as a water-soluble Na-salt, is subject to Na+/Ca2+ exchange by the Ca2+ from the cement, resulting in the formation of a water-rich coacervate when added to the cement surface, especially to the surface of bacteria-containing cement/concrete samples. The addition of polyP in low concentrations (<1% on weight basis for the solids) not only accelerated the hardening of cement/concrete but also the healing of microcracks present in the material. The results suggest that long-chain polyP is a promising additive that increases the self-healing capacity of cement by mimicking a bacteria-mediated natural mechanism.

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Section: Methodsmentioning

confidence: 99%

Transformation of Construction Cement to a Self-Healing Hybrid Binder

Müller

Tolba

Wang

et al. 2019

IJMS

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“…Bacterial alkaline phosphatases are more heat stable than those of animal and plant origin, and researchers have shown the effective use of heat treatment step to differeniate bacterial alkaline phosphatases from those of both plant 43 and animal origin 44 when testing foods and could be implemented as part of our assay. There a several methods which can be used to reduce this potential interferance.…”

Section: Future Applications: Food and Water Testingmentioning

confidence: 99%

“…There a several methods which can be used to reduce this potential interferance. Bacterial alkaline phosphatases are more heat stable than those of animal and plant origin, and researchers have shown the effective use of heat treatment step to differeniate bacterial alkaline phosphatases from those of both plant 43 and animal origin 44 when testing foods and could be implemented as part of our assay.…”

Section: Future Applications: Food and Water Testingmentioning

confidence: 99%

Phage & phosphatase: a novel phage-based probe for rapid, multi-platform detection of bacteria

Alcaine

Pacitto

Sela

et al. 2015

Analyst

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Genetic engineering of bacteriophages allows for the development of rapid, highly specific, and easily manufactured probes for the detection of bacterial pathogens. A challenge for novel probes is the ease of their adoption in real world laboratories. We have engineered the bacteriophage T7, which targets Escherichia coli, to carry the alkaline phosphatase gene, phoA. This inclusion results in phoA overexpression following phage infection of E. coli. Alkaline phosphatase is commonly used in a wide range of diagnostics, and thus a signal produced by our phage-based probe could be detected using common laboratory equipment. Our work demonstrates the successful: (i) modification of T7 phage to carry phoA; (ii) overexpression of alkaline phosphatase in E. coli; and (iii) detection of this T7-induced alkaline phosphatase activity using commercially available colorimetric and chemilumiscent methods. Furthermore, we demonstrate the application of our phage-based probe to rapidly detect low levels of bacteria and discern the antibiotic resistance of E. coli isolates. Using our bioengineered phage-based probe we were able to detect 10(3) CFU per mL of E. coli in 6 hours using a chemiluminescent substrate and 10(4) CFU per mL within 7.5 hours using a colorimetric substrate. We also show the application of this phage-based probe for antibiotic resistance testing. We were able to determine whether an E. coli isolate was resistant to ampicillin within 4.5 hours using chemiluminescent substrate and within 6 hours using a colorimetric substrate. This phage-based scheme could be readily adopted in labs without significant capital investments and can be translated to other phage-bacteria pairs for further detection.

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The Use of Bacterial Alkaline Phosphatase Assay for Rapid Monitoring of Bacterial Counts on Spinach

Cited by 2 publications

References 11 publications

Transformation of Construction Cement to a Self-Healing Hybrid Binder

Transformation of Construction Cement to a Self-Healing Hybrid Binder

Phage & phosphatase: a novel phage-based probe for rapid, multi-platform detection of bacteria

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