The use of avidin to accelerate blood clearance of biotinylated immunogobulins

Sinitsyn, V. V.; Mamontova, Anna G.; Chekneva, E. E.; Shnyra, Alexander; Домогатский, С. П.

doi:10.1007/bf00841437

Cited by 3 publications

(2 citation statements)

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“…Specific highaffinity interaction between these compounds has been employed in various immobilization systems (as 'cross-linking' and 'staining' agents etc.). Some researchers attempted to apply the avidin-biotin pair in vivo to the gamma-scintigraphic technique [17], acceleration of the clearance of labelled proteins in the circulation [18,19] and drug targeting [20]. However, the effects of biotin-avidin or of components carrying this pair on the effector systems of the organism are poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Avidin acylation prevents the complement-dependent lysis of avidin-carrying erythrocytes

Muzykantov

Smirnov

Samokhin

1991

Biochemical Journal

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Non-covalent binding of avidin to biotinylated erythrocytes results in complement-dependent haemolysis. Biotinylated erythrocytes, as well as native cells, are not lysed by complement. Complement activation requires a tight contact between avidin and the erythrocyte membrane, since avidin does not in itself activate complement and does not inhibit lysis of sensitized sheep erythrocytes. The efficiency of haemolysis depends on avidin's surface density. When the avidin concentration in the reaction mixture is less than 15 micrograms/ml, erythrocyte lysis is not induced. However, the attachment of biotinylated antibodies to avidin-carrying erythrocytes decreases dramatically. Acylation of avidin with succinic anhydride strongly decreases its ability to induce complement-dependent haemolysis. However, the ability of avidin to cross-link the biotin-containing structures decreases after acylation. A 50% modification of avidin by succinic anhydride (pI about 7.0) allows preparation of 'immunoerythrocytes', which retain their affinity to antigen and stability in the presence of complement.

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Section: Discussionmentioning

confidence: 99%